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Article: Proton permeability and lipid dynamics of gastric and duodenal apical membrane vesicles

TitleProton permeability and lipid dynamics of gastric and duodenal apical membrane vesicles
Authors
Issue Date1989
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1989, v. 256 n. 3, p. 19/3 How to Cite?
AbstractThe passive proton permeability (P(net)) of apical membrane vesicles from rabbit parietal cells (gastric) and duodenal and renal cortical brush-border membranes (BBM) was determined by acridine orange fluorescence quenching. Values of P(net) were found to be gastric (4 x 10-4 cm/s) < duodenal (10-3 cm/s) << renal (10-2 cm/s). Arrhenius plots of the temperature profile of proton permeation of gastric vesicles was linear, whereas that of duodenal BBM displayed a discontinuity at 30-33°C. Alcohols (octyl, benzyl, ethyl) increased P(net) in a concentration-dependent manner, with efficacy related to their oil-water partition coefficients. In a parallel series of experiments, structural parameters of the vesicle membrane lipids (fluidity) were monitored from both the steady-state and time-resolved fluorescence anisotropy of diphenylhexatriene. Fluidity of the membranes was unrelated to P(net) (renal ≃ duodenal < gastric). Gastric vesicles demonstrated a linear Arrhenius plot of temperature dependence for fluidity, whereas duodenal BBM demonstrated a discontinuity. Membrane fluidity of gastric and duodenal vesicles was increased by alcohols, with the same potency as for P(net), and these two variables were significantly correlated after perturbation with alcohols. Thus the fluidity of the lipid bilayer is not the major determinant of P(net), but alteration of its structural parameters, as reflected by fluidity, produces parallel changes in P(net).
Persistent Identifierhttp://hdl.handle.net/10722/171537
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWilkes, JMen_US
dc.contributor.authorBallard, HJen_US
dc.contributor.authorDryden, DTFen_US
dc.contributor.authorHirst, BHen_US
dc.date.accessioned2012-10-30T06:15:35Z-
dc.date.available2012-10-30T06:15:35Z-
dc.date.issued1989en_US
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 1989, v. 256 n. 3, p. 19/3en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/171537-
dc.description.abstractThe passive proton permeability (P(net)) of apical membrane vesicles from rabbit parietal cells (gastric) and duodenal and renal cortical brush-border membranes (BBM) was determined by acridine orange fluorescence quenching. Values of P(net) were found to be gastric (4 x 10-4 cm/s) < duodenal (10-3 cm/s) << renal (10-2 cm/s). Arrhenius plots of the temperature profile of proton permeation of gastric vesicles was linear, whereas that of duodenal BBM displayed a discontinuity at 30-33°C. Alcohols (octyl, benzyl, ethyl) increased P(net) in a concentration-dependent manner, with efficacy related to their oil-water partition coefficients. In a parallel series of experiments, structural parameters of the vesicle membrane lipids (fluidity) were monitored from both the steady-state and time-resolved fluorescence anisotropy of diphenylhexatriene. Fluidity of the membranes was unrelated to P(net) (renal ≃ duodenal < gastric). Gastric vesicles demonstrated a linear Arrhenius plot of temperature dependence for fluidity, whereas duodenal BBM demonstrated a discontinuity. Membrane fluidity of gastric and duodenal vesicles was increased by alcohols, with the same potency as for P(net), and these two variables were significantly correlated after perturbation with alcohols. Thus the fluidity of the lipid bilayer is not the major determinant of P(net), but alteration of its structural parameters, as reflected by fluidity, produces parallel changes in P(net).en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Membrane - Physiologyen_US
dc.subject.meshCell Membrane Permeabilityen_US
dc.subject.meshDuodenum - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastric Fundus - Physiologyen_US
dc.subject.meshGastric Mucosa - Physiologyen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshIntestinal Mucosa - Physiologyen_US
dc.subject.meshKidney Cortex - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Fluidityen_US
dc.subject.meshMembrane Lipids - Physiologyen_US
dc.subject.meshMicrovilli - Physiologyen_US
dc.subject.meshMucous Membrane - Physiologyen_US
dc.subject.meshParietal Cells, Gastric - Physiologyen_US
dc.subject.meshProtonsen_US
dc.subject.meshRabbitsen_US
dc.subject.meshThermodynamicsen_US
dc.titleProton permeability and lipid dynamics of gastric and duodenal apical membrane vesiclesen_US
dc.typeArticleen_US
dc.identifier.emailBallard, HJ:ballard@hkucc.hku.hken_US
dc.identifier.authorityBallard, HJ=rp00367en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2538075-
dc.identifier.scopuseid_2-s2.0-0024539836en_US
dc.identifier.volume256en_US
dc.identifier.issue3en_US
dc.identifier.spage19/3en_US
dc.identifier.isiWOS:A1989T693800063-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWilkes, JM=7102513233en_US
dc.identifier.scopusauthoridBallard, HJ=7005286310en_US
dc.identifier.scopusauthoridDryden, DTF=7004366661en_US
dc.identifier.scopusauthoridHirst, BH=7005256376en_US

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