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Article: A membrane potential-sensitive Na+-H+ exchange system in flagella isolated from sea urchin spermatozoa

TitleA membrane potential-sensitive Na+-H+ exchange system in flagella isolated from sea urchin spermatozoa
Authors
Issue Date1984
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1984, v. 259 n. 24, p. 15315-15319 How to Cite?
AbstractSea urchin sperm motility is activated by a Na+-dependent increase of internal pH. A flagellar preparation was used in the present study to investigate this ionic mechanism. Using 22Na and a pH electrode, the stoichiometry of Na+ uptake to H+ release in the isolated flagella was found to be 1.09 ± 0.11. Reversing the Na+ gradient induced reacidification of the intraflagellar pH as measured by [14C]methylamine, while reversal of the H+ gradient resulted in a Na+ efflux. Furthermore, a parallel inhibition of both ionic movements was observed with increasing external [K+]. These results indicate that Na+ and H+ are coupled through an exchanger. Measurements of the membrane potential (ψ) with [3H]tetraphenylphosphonium showed depolarization by K+, suggesting its inhibitory effect on the exchanger is through changes in ψ. This is further supported by the following experiments. (a) Cs+ by itself had little effect on either ψ or the Na+/H+ exchange, but in the presence of the ionophore valinomycin it depolarized ψ and inhibited the exchange. (b) Tetraphenylphosphonium a highly permeant cation, at 2.5 mM caused depolarization and inhibition of the exchange, and these effects were reversible by repolarization of ψ with valinomycin. The inhibitory effect of depolarization was not due to the electrogenicity of the exchange since both directions of the exchange were inhibited. It is proposed that the flagellar exchange is basically a electroneutral process but has a charged regulatory component (a gate or a conformational change) which confers the observed potential sensitivity.
Persistent Identifierhttp://hdl.handle.net/10722/171491
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, HCen_US
dc.date.accessioned2012-10-30T06:15:25Z-
dc.date.available2012-10-30T06:15:25Z-
dc.date.issued1984en_US
dc.identifier.citationJournal Of Biological Chemistry, 1984, v. 259 n. 24, p. 15315-15319en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/171491-
dc.description.abstractSea urchin sperm motility is activated by a Na+-dependent increase of internal pH. A flagellar preparation was used in the present study to investigate this ionic mechanism. Using 22Na and a pH electrode, the stoichiometry of Na+ uptake to H+ release in the isolated flagella was found to be 1.09 ± 0.11. Reversing the Na+ gradient induced reacidification of the intraflagellar pH as measured by [14C]methylamine, while reversal of the H+ gradient resulted in a Na+ efflux. Furthermore, a parallel inhibition of both ionic movements was observed with increasing external [K+]. These results indicate that Na+ and H+ are coupled through an exchanger. Measurements of the membrane potential (ψ) with [3H]tetraphenylphosphonium showed depolarization by K+, suggesting its inhibitory effect on the exchanger is through changes in ψ. This is further supported by the following experiments. (a) Cs+ by itself had little effect on either ψ or the Na+/H+ exchange, but in the presence of the ionophore valinomycin it depolarized ψ and inhibited the exchange. (b) Tetraphenylphosphonium a highly permeant cation, at 2.5 mM caused depolarization and inhibition of the exchange, and these effects were reversible by repolarization of ψ with valinomycin. The inhibitory effect of depolarization was not due to the electrogenicity of the exchange since both directions of the exchange were inhibited. It is proposed that the flagellar exchange is basically a electroneutral process but has a charged regulatory component (a gate or a conformational change) which confers the observed potential sensitivity.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarrier Proteins - Metabolismen_US
dc.subject.meshFlagella - Drug Effects - Metabolismen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentialsen_US
dc.subject.meshOnium Compounds - Metabolismen_US
dc.subject.meshOrganophosphorus Compounds - Metabolismen_US
dc.subject.meshSea Urchinsen_US
dc.subject.meshSodium - Metabolismen_US
dc.subject.meshSodium-Hydrogen Antiporteren_US
dc.subject.meshSperm Motilityen_US
dc.subject.meshSpermatozoa - Metabolismen_US
dc.subject.meshValinomycin - Pharmacologyen_US
dc.titleA membrane potential-sensitive Na+-H+ exchange system in flagella isolated from sea urchin spermatozoaen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6096367-
dc.identifier.scopuseid_2-s2.0-0021690494en_US
dc.identifier.volume259en_US
dc.identifier.issue24en_US
dc.identifier.spage15315en_US
dc.identifier.epage15319en_US
dc.identifier.isiWOS:A1984TX27200053-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US

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