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- Publisher Website: 10.1016/0005-2736(78)90336-X
- Scopus: eid_2-s2.0-0017885019
- PMID: 25082
- WOS: WOS:A1978EW26000013
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Article: A study of H+ transport in gastric microsomal vesicles using fluorescent probes
Title | A study of H+ transport in gastric microsomal vesicles using fluorescent probes |
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Authors | |
Issue Date | 1978 |
Citation | Biochimica Et Biophysica Acta, 1978, v. 508 n. 2, p. 339-356 How to Cite? |
Abstract | Fluorescent amines, 9-aminoacridine, acridine orange and quinacrine, were used as probes for a pH gradient (ΔpH) across gastric microsomal vesicles. Analysis of probe uptake data indicates that 9-aminoacridine distributes across the membrane as a weak base in accordance with the ΔpH. On the other hand, acridine orange and quinacrine show characteristics of binding to membrane sites in addition to accumulation in response to ΔpH. A discussion of the advantages and limitations of the probes is presented. Application of these probes to pig gastric microsomal vesicles indicates that the K +-stimulated ATPase is responsible for the transport of H + into the vesicles and thus develops a ΔpH across the membrane. The ΔpH generated by the K +-ATPase has a definite requirement for internal K +. The proton gradient can be discharged slowly after ATP depletion or rapidly either by detergent disruption of the vesicles or by increasing their leakiness using both H + and K + ionophores. On the other hand, the sole use of the K + ionophore, valinomycin, stimulates the ATP-induced formation of ΔpH by increasing the availability of K + to internal sites. This stimulation by valinomycin requires the presence of permeable anions like Cl -. Analysis of the Cl - requirement indicates that in the presence of valinomycin the net effect is the accumulation of HCl inside the gastric vesicles. With an external pH of 7.0, the ATP-generated ΔpH was calculated to be from 4 to 4.5 pH units. The results are consistent with the hypothesis that the K +-stimulated ATPase drives a K +/H + exchange across the gastric vesicles. Since other lines of evidence suggest that these gastric microsomes are derived from the tubulovesicular system of the oxyntic cell, the participation of the ATP-driven transport processes in gastric HCl secretion is of interest. |
Persistent Identifier | http://hdl.handle.net/10722/171467 |
ISSN | 2020 SCImago Journal Rankings: 1.204 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, HC | en_US |
dc.contributor.author | Forte, JG | en_US |
dc.date.accessioned | 2012-10-30T06:15:18Z | - |
dc.date.available | 2012-10-30T06:15:18Z | - |
dc.date.issued | 1978 | en_US |
dc.identifier.citation | Biochimica Et Biophysica Acta, 1978, v. 508 n. 2, p. 339-356 | en_US |
dc.identifier.issn | 0006-3002 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171467 | - |
dc.description.abstract | Fluorescent amines, 9-aminoacridine, acridine orange and quinacrine, were used as probes for a pH gradient (ΔpH) across gastric microsomal vesicles. Analysis of probe uptake data indicates that 9-aminoacridine distributes across the membrane as a weak base in accordance with the ΔpH. On the other hand, acridine orange and quinacrine show characteristics of binding to membrane sites in addition to accumulation in response to ΔpH. A discussion of the advantages and limitations of the probes is presented. Application of these probes to pig gastric microsomal vesicles indicates that the K +-stimulated ATPase is responsible for the transport of H + into the vesicles and thus develops a ΔpH across the membrane. The ΔpH generated by the K +-ATPase has a definite requirement for internal K +. The proton gradient can be discharged slowly after ATP depletion or rapidly either by detergent disruption of the vesicles or by increasing their leakiness using both H + and K + ionophores. On the other hand, the sole use of the K + ionophore, valinomycin, stimulates the ATP-induced formation of ΔpH by increasing the availability of K + to internal sites. This stimulation by valinomycin requires the presence of permeable anions like Cl -. Analysis of the Cl - requirement indicates that in the presence of valinomycin the net effect is the accumulation of HCl inside the gastric vesicles. With an external pH of 7.0, the ATP-generated ΔpH was calculated to be from 4 to 4.5 pH units. The results are consistent with the hypothesis that the K +-stimulated ATPase drives a K +/H + exchange across the gastric vesicles. Since other lines of evidence suggest that these gastric microsomes are derived from the tubulovesicular system of the oxyntic cell, the participation of the ATP-driven transport processes in gastric HCl secretion is of interest. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Biochimica et Biophysica Acta | en_US |
dc.subject.mesh | Acridines - Metabolism | en_US |
dc.subject.mesh | Adenosine Triphosphatases - Metabolism | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Biological Transport | en_US |
dc.subject.mesh | Fluorescent Dyes | en_US |
dc.subject.mesh | Gastric Mucosa - Physiology | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Membranes - Physiology | en_US |
dc.subject.mesh | Microsomes - Physiology | en_US |
dc.subject.mesh | Potassium - Metabolism | en_US |
dc.subject.mesh | Quinacrine - Metabolism | en_US |
dc.subject.mesh | Spectrometry, Fluorescence | en_US |
dc.title | A study of H+ transport in gastric microsomal vesicles using fluorescent probes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, HC:leehc@hku.hk | en_US |
dc.identifier.authority | Lee, HC=rp00545 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0005-2736(78)90336-X | - |
dc.identifier.pmid | 25082 | - |
dc.identifier.scopus | eid_2-s2.0-0017885019 | en_US |
dc.identifier.volume | 508 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 339 | en_US |
dc.identifier.epage | 356 | en_US |
dc.identifier.isi | WOS:A1978EW26000013 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Lee, HC=26642959100 | en_US |
dc.identifier.scopusauthorid | Forte, JG=26425932500 | en_US |
dc.identifier.issnl | 0006-3002 | - |