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- Publisher Website: 10.1152/ajpheart.01068.2009
- Scopus: eid_2-s2.0-77949760342
- PMID: 20118407
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Article: Calcium-independent phospholipase A2 plays a key role in the endothelium-dependent contractions to acetylcholine in the aorta of the spontaneously hypertensive rat
Title | Calcium-independent phospholipase A2 plays a key role in the endothelium-dependent contractions to acetylcholine in the aorta of the spontaneously hypertensive rat |
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Authors | |
Keywords | Endothelium-derived contracting factor Prostacyclin Thromboxane A 2 |
Issue Date | 2010 |
Publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/ |
Citation | American Journal Of Physiology - Heart And Circulatory Physiology, 2010, v. 298 n. 4, p. H1260-H1266 How to Cite? |
Abstract | Phospholipase A2 (PLA2), a regulatory enzyme found in most mammalian cells, catalyzes the breakdown of membrane phospholipids to arachidonic acid. There are two major cytosolic types of the enzyme, calcium-dependent (cPLA2) and calcium-independent (iPLA2) PLA2. The present study investigated whether or not iPLA2 plays a role in the endothelium-dependent contractions of the aorta of the spontaneously hypertensive rat and its normotensive counterpart, the Wistar-Kyoto rat. The presence of iPLA2 in the endothelial cells was identified by using immunochemistry and immunoblotting. Aortic rings with and without the endothelium were suspended in organ chambers for isometric tension recording. The production of prostanoids was measured by using enzyme immunoassay kits. iPLA2 was densely distributed in endothelial cells of the aorta of both strains. At 3 x 10-6 M, the selective iPLA 2 inhibitor, bromoenol lactone (BEL), abrogated endothelium-dependent contractions induced by acetylcholine but not those evoked by the calcium ionophore A-23187. The effects of BEL were similar in the aortae of Wistar-Kyoto and spontaneously hypertensive rats. The nonselective PLA2 inhibitor quinacrine abolished the contractions triggered by both acetylcholine and A-23187, whereas the store-operated calcium channel inhibitor SKF-96365 prevented only the acetylcholine-induced contraction. The acetylcholine- but not the A-23187-induced release of 6-keto prostaglandin F1α was inhibited by BEL. The release of thromboxane B2 by either acetylcholine or A-23187 was not affected by BEL. In conclusion, iPLA 2 plays a substantial role in the generation of endothelium-derived contracting factor evoked by acetylcholine. Copyright © 2010 the American Physiological Society. |
Persistent Identifier | http://hdl.handle.net/10722/171399 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.452 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, MSK | en_US |
dc.contributor.author | Man, RYK | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:13:53Z | - |
dc.date.available | 2012-10-30T06:13:53Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.citation | American Journal Of Physiology - Heart And Circulatory Physiology, 2010, v. 298 n. 4, p. H1260-H1266 | en_US |
dc.identifier.issn | 0363-6135 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171399 | - |
dc.description.abstract | Phospholipase A2 (PLA2), a regulatory enzyme found in most mammalian cells, catalyzes the breakdown of membrane phospholipids to arachidonic acid. There are two major cytosolic types of the enzyme, calcium-dependent (cPLA2) and calcium-independent (iPLA2) PLA2. The present study investigated whether or not iPLA2 plays a role in the endothelium-dependent contractions of the aorta of the spontaneously hypertensive rat and its normotensive counterpart, the Wistar-Kyoto rat. The presence of iPLA2 in the endothelial cells was identified by using immunochemistry and immunoblotting. Aortic rings with and without the endothelium were suspended in organ chambers for isometric tension recording. The production of prostanoids was measured by using enzyme immunoassay kits. iPLA2 was densely distributed in endothelial cells of the aorta of both strains. At 3 x 10-6 M, the selective iPLA 2 inhibitor, bromoenol lactone (BEL), abrogated endothelium-dependent contractions induced by acetylcholine but not those evoked by the calcium ionophore A-23187. The effects of BEL were similar in the aortae of Wistar-Kyoto and spontaneously hypertensive rats. The nonselective PLA2 inhibitor quinacrine abolished the contractions triggered by both acetylcholine and A-23187, whereas the store-operated calcium channel inhibitor SKF-96365 prevented only the acetylcholine-induced contraction. The acetylcholine- but not the A-23187-induced release of 6-keto prostaglandin F1α was inhibited by BEL. The release of thromboxane B2 by either acetylcholine or A-23187 was not affected by BEL. In conclusion, iPLA 2 plays a substantial role in the generation of endothelium-derived contracting factor evoked by acetylcholine. Copyright © 2010 the American Physiological Society. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Heart and Circulatory Physiology | en_US |
dc.subject | Endothelium-derived contracting factor | - |
dc.subject | Prostacyclin | - |
dc.subject | Thromboxane A 2 | - |
dc.subject.mesh | Acetylcholine - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aorta - Enzymology | en_US |
dc.subject.mesh | Calcimycin - Pharmacology | en_US |
dc.subject.mesh | Disease Models, Animal | en_US |
dc.subject.mesh | Endothelium, Vascular - Enzymology | en_US |
dc.subject.mesh | Hypertension - Enzymology - Physiopathology | en_US |
dc.subject.mesh | Imidazoles - Pharmacology | en_US |
dc.subject.mesh | Ionophores - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects - Physiology | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Enzymology | en_US |
dc.subject.mesh | Naphthalenes - Pharmacology | en_US |
dc.subject.mesh | Phosphodiesterase Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Phospholipases A2, Calcium-Independent - Metabolism | en_US |
dc.subject.mesh | Pyrones - Pharmacology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Inbred Shr | en_US |
dc.subject.mesh | Rats, Inbred Wky | en_US |
dc.subject.mesh | Vasodilator Agents - Pharmacology | en_US |
dc.title | Calcium-independent phospholipase A2 plays a key role in the endothelium-dependent contractions to acetylcholine in the aorta of the spontaneously hypertensive rat | en_US |
dc.type | Article | en_US |
dc.identifier.email | Man, RYK:rykman@hkucc.hku.hk | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Man, RYK=rp00236 | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1152/ajpheart.01068.2009 | en_US |
dc.identifier.pmid | 20118407 | - |
dc.identifier.scopus | eid_2-s2.0-77949760342 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77949760342&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 298 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | H1260 | en_US |
dc.identifier.epage | H1266 | en_US |
dc.identifier.isi | WOS:000275768600017 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wong, MSK=23483301500 | en_US |
dc.identifier.scopusauthorid | Man, RYK=7004986435 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0363-6135 | - |