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Article: Characterization of a charybdotoxin-sensitive intermediate conductance Ca 2+-activated K + channel in porcine coronary endothelium: Relevance to EDHF

TitleCharacterization of a charybdotoxin-sensitive intermediate conductance Ca 2+-activated K + channel in porcine coronary endothelium: Relevance to EDHF
Authors
Issue Date2002
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 2002, v. 137 n. 8, p. 1346-1354 How to Cite?
Abstract1. This study characterizes the K + channel(s) underlying charybdotoxin-sensitive hyperpolarization of porcine coronary artery endothelium. 2. Two forms of current-voltage (I/V) relationship were evident in whole-cell patch-clamp recordings of freshly-isolated endothelial cells. In both cell types, iberiotoxin (100 nM) inhibited a current active only at potentials over +50 mV. In the presence of iberiotoxin, charybdotoxin (100 nM) produced a large inhibition in 38% of cells and altered the form of the I/V relationship. In the remaining cells, charybdotoxin also inhibited a current but did not alter the form. 3. Single-channel, outside-out patch recordings revealed a 17.1 ± 0.4 pS conductance. Pipette solutions containing 100, 250 and 500 nM free Ca 2+ demonstrated that the open probability was increased by Ca 2+. This channel was blocked by charybdotoxin but not by iberiotoxin or apamin. 4. Hyperpolarizations of intact endothelium elicited by substance P (100 nM; 26.1 ± 0.7 mV) were reduced by apamin (100 nM; 17.0 ± 1.8 mV) whereas those to 1-ethyl-2-benzimidazolinone (1-EBIO, 600 μM, 21.0 ± 0.3 mV) were unaffected (21.7 ± 0.8 mV). Substance P, bradykinin (100 nM) and 1-EBIO evoked charybdotoxin-sensitive, iberiotoxin-insensitive whole-cell perforated-patch currents. 5. A porcine homologue of the intermediate-conductance Ca 2+-activated K + channel (IK1) was identified in endothelial cells. 6 In conclusion, porcine coronary artery endothelial cells express an intermediate-conductance Ca 2+-activated K + channel and the IK1 gene product. This channel is opened by activation of the EDHF pathway and likely mediates the charybdotoxin-sensitive component of the EDHF response.
Persistent Identifierhttp://hdl.handle.net/10722/171312
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBychkov, Ren_US
dc.contributor.authorBurnham, MPen_US
dc.contributor.authorRichards, GRen_US
dc.contributor.authorEdwards, Gen_US
dc.contributor.authorWeston, AHen_US
dc.contributor.authorFélétou, Men_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:13:20Z-
dc.date.available2012-10-30T06:13:20Z-
dc.date.issued2002en_US
dc.identifier.citationBritish Journal Of Pharmacology, 2002, v. 137 n. 8, p. 1346-1354en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/171312-
dc.description.abstract1. This study characterizes the K + channel(s) underlying charybdotoxin-sensitive hyperpolarization of porcine coronary artery endothelium. 2. Two forms of current-voltage (I/V) relationship were evident in whole-cell patch-clamp recordings of freshly-isolated endothelial cells. In both cell types, iberiotoxin (100 nM) inhibited a current active only at potentials over +50 mV. In the presence of iberiotoxin, charybdotoxin (100 nM) produced a large inhibition in 38% of cells and altered the form of the I/V relationship. In the remaining cells, charybdotoxin also inhibited a current but did not alter the form. 3. Single-channel, outside-out patch recordings revealed a 17.1 ± 0.4 pS conductance. Pipette solutions containing 100, 250 and 500 nM free Ca 2+ demonstrated that the open probability was increased by Ca 2+. This channel was blocked by charybdotoxin but not by iberiotoxin or apamin. 4. Hyperpolarizations of intact endothelium elicited by substance P (100 nM; 26.1 ± 0.7 mV) were reduced by apamin (100 nM; 17.0 ± 1.8 mV) whereas those to 1-ethyl-2-benzimidazolinone (1-EBIO, 600 μM, 21.0 ± 0.3 mV) were unaffected (21.7 ± 0.8 mV). Substance P, bradykinin (100 nM) and 1-EBIO evoked charybdotoxin-sensitive, iberiotoxin-insensitive whole-cell perforated-patch currents. 5. A porcine homologue of the intermediate-conductance Ca 2+-activated K + channel (IK1) was identified in endothelial cells. 6 In conclusion, porcine coronary artery endothelial cells express an intermediate-conductance Ca 2+-activated K + channel and the IK1 gene product. This channel is opened by activation of the EDHF pathway and likely mediates the charybdotoxin-sensitive component of the EDHF response.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAmino Acid Sequence - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Factors - Physiologyen_US
dc.subject.meshCharybdotoxin - Pharmacologyen_US
dc.subject.meshCoronary Vessels - Cytology - Drug Effects - Physiologyen_US
dc.subject.meshEndothelium, Vascular - Cytology - Drug Effects - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPotassium Channels, Calcium-Activated - Genetics - Physiologyen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshSwineen_US
dc.titleCharacterization of a charybdotoxin-sensitive intermediate conductance Ca 2+-activated K + channel in porcine coronary endothelium: Relevance to EDHFen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.bjp.0705057en_US
dc.identifier.pmid12466245-
dc.identifier.scopuseid_2-s2.0-12244267091en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12244267091&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume137en_US
dc.identifier.issue8en_US
dc.identifier.spage1346en_US
dc.identifier.epage1354en_US
dc.identifier.isiWOS:000179847100024-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridBychkov, R=6603668408en_US
dc.identifier.scopusauthoridBurnham, MP=7004848578en_US
dc.identifier.scopusauthoridRichards, GR=7201583688en_US
dc.identifier.scopusauthoridEdwards, G=7402317535en_US
dc.identifier.scopusauthoridWeston, AH=7102913361en_US
dc.identifier.scopusauthoridFélétou, M=7006461826en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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