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- Publisher Website: 10.1095/biolreprod.102.010017
- Scopus: eid_2-s2.0-0037407445
- PMID: 12606488
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Article: Synergistic effects of cystic fibrosis transmembrane conductance regulator and aquaporin-9 in the rat epididymis
Title | Synergistic effects of cystic fibrosis transmembrane conductance regulator and aquaporin-9 in the rat epididymis |
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Authors | |
Keywords | Aquaporin-9 Cystic fibrosis transmembrane conductance regulator Epididymis |
Issue Date | 2003 |
Publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ |
Citation | Biology Of Reproduction, 2003, v. 68 n. 5, p. 1505-1510 How to Cite? |
Abstract | The cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin-9 (AQP-9) are present in the luminal membrane of the epididymis, where they play an important role in formation of the epididymal fluid. Evidence is accumulating that CFTR regulates other membrane transport proteins besides functioning as a cAMP-activated chloride channel. We have explored the possible interaction between epididymal CFTR and AQP-9 by cloning them from the rat epididymis and expressing them in Xenopus oocytes. The effects of the expressed proteins on oocyte water permeability were studied by immersing oocytes in a hypo-osmotic solution, and the ensuing water flow was measured using a gravimetric method. The results show that AQP-9 alone caused an increase in oocyte water permeability, which could be further potentiated by CFTR. This potentiation was markedly reduced by phloretin and Ionidamine (inhibitors of AQP-9 and CFTR, respectively). The regulation of water permeability by CFTR was also demonstrated in intact rat epididymis luminally perfused with a hypo-osmotic solution. Osmotic water reabsorption across the epididymal tubule was reduced by phloretin and Ionidamine. Elevation of intracellular cAMP with 3-isobutyl-1-methylxanthine increased osmotic water permeability, whereas inhibiting protein kinase A with H-89 (N-(2- [p-bromocinnamylamino]ethyl)-5-isoquinoline sulfonamide hydrochloride) reduced it. These results are consistent with a role for CFTR in controlling water permeability in the epididymis in vivo. We conclude that this additional role of CFTR in controlling water permeability may have an impact on the genetic disease cystic fibrosis, in which men with a mutated CFTR gene have abnormal epididymis and infertility. |
Persistent Identifier | http://hdl.handle.net/10722/171292 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.022 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheung, KH | en_US |
dc.contributor.author | Leung, CT | en_US |
dc.contributor.author | Leung, GPH | en_US |
dc.contributor.author | Wong, PYD | en_US |
dc.date.accessioned | 2012-10-30T06:13:13Z | - |
dc.date.available | 2012-10-30T06:13:13Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Biology Of Reproduction, 2003, v. 68 n. 5, p. 1505-1510 | en_US |
dc.identifier.issn | 0006-3363 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171292 | - |
dc.description.abstract | The cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin-9 (AQP-9) are present in the luminal membrane of the epididymis, where they play an important role in formation of the epididymal fluid. Evidence is accumulating that CFTR regulates other membrane transport proteins besides functioning as a cAMP-activated chloride channel. We have explored the possible interaction between epididymal CFTR and AQP-9 by cloning them from the rat epididymis and expressing them in Xenopus oocytes. The effects of the expressed proteins on oocyte water permeability were studied by immersing oocytes in a hypo-osmotic solution, and the ensuing water flow was measured using a gravimetric method. The results show that AQP-9 alone caused an increase in oocyte water permeability, which could be further potentiated by CFTR. This potentiation was markedly reduced by phloretin and Ionidamine (inhibitors of AQP-9 and CFTR, respectively). The regulation of water permeability by CFTR was also demonstrated in intact rat epididymis luminally perfused with a hypo-osmotic solution. Osmotic water reabsorption across the epididymal tubule was reduced by phloretin and Ionidamine. Elevation of intracellular cAMP with 3-isobutyl-1-methylxanthine increased osmotic water permeability, whereas inhibiting protein kinase A with H-89 (N-(2- [p-bromocinnamylamino]ethyl)-5-isoquinoline sulfonamide hydrochloride) reduced it. These results are consistent with a role for CFTR in controlling water permeability in the epididymis in vivo. We conclude that this additional role of CFTR in controlling water permeability may have an impact on the genetic disease cystic fibrosis, in which men with a mutated CFTR gene have abnormal epididymis and infertility. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ | en_US |
dc.relation.ispartof | Biology of Reproduction | en_US |
dc.subject | Aquaporin-9 | - |
dc.subject | Cystic fibrosis transmembrane conductance regulator | - |
dc.subject | Epididymis | - |
dc.subject.mesh | Absorption | en_US |
dc.subject.mesh | Algorithms | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aquaporins - Antagonists & Inhibitors - Metabolism | en_US |
dc.subject.mesh | Cell Membrane Permeability - Drug Effects | en_US |
dc.subject.mesh | Chloride Channels - Metabolism | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Cyclic Amp-Dependent Protein Kinases - Metabolism | en_US |
dc.subject.mesh | Cystic Fibrosis Transmembrane Conductance Regulator - Antagonists & Inhibitors - Metabolism | en_US |
dc.subject.mesh | Epididymis - Cytology - Metabolism | en_US |
dc.subject.mesh | Indazoles - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Microinjections | en_US |
dc.subject.mesh | Oocytes - Metabolism | en_US |
dc.subject.mesh | Osmosis | en_US |
dc.subject.mesh | Phloretin - Pharmacology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Xenopus Laevis | en_US |
dc.title | Synergistic effects of cystic fibrosis transmembrane conductance regulator and aquaporin-9 in the rat epididymis | en_US |
dc.type | Article | en_US |
dc.identifier.email | Cheung, KH:kingho.cheung@hku.hk | en_US |
dc.identifier.email | Leung, GPH:gphleung@hkucc.hku.hk | en_US |
dc.identifier.authority | Cheung, KH=rp01463 | en_US |
dc.identifier.authority | Leung, GPH=rp00234 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1095/biolreprod.102.010017 | en_US |
dc.identifier.pmid | 12606488 | - |
dc.identifier.scopus | eid_2-s2.0-0037407445 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037407445&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 68 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 1505 | en_US |
dc.identifier.epage | 1510 | en_US |
dc.identifier.isi | WOS:000182589700005 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Cheung, KH=14007487800 | en_US |
dc.identifier.scopusauthorid | Leung, CT=7402612664 | en_US |
dc.identifier.scopusauthorid | Leung, GPH=35963668200 | en_US |
dc.identifier.scopusauthorid | Wong, PYD=7403980262 | en_US |
dc.identifier.issnl | 0006-3363 | - |