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Article: Synthesis of collagen is dysregulated in cultured fibroblasts derived from skin of subjects with varicose veins as it is in venous smooth muscle cells

TitleSynthesis of collagen is dysregulated in cultured fibroblasts derived from skin of subjects with varicose veins as it is in venous smooth muscle cells
Authors
Issue Date2002
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org
Citation
Circulation, 2002, v. 106 n. 4, p. 479-483 How to Cite?
AbstractBackground - The dilatation and tortuosity observed in varicose veins provide evidence for progressive venous wall remodeling associated with abnormalities of smooth muscle cells and extracellular matrix. The present study was designed to examine if the phenotypic modulations observed in the venous smooth muscle cells of patients with varicose veins were also present in their dermal fibroblasts. Methods and Results - Collagen type I (collagen I), type III (collagen III), and type V (collagen V) were compared in dermal fibroblasts derived from the skin of control subjects and patients with varicose veins. The synthesis of collagen I, the release of its metabolites, and the expression of its mRNA were increased in fibroblasts from patients with varicose veins, whereas the synthesis of collagen III was decreased but not correlated with a decrease in mRNA expression and in metabolite release. Matrix metalloproteinases (MMP1, 2, 7, 8, 9, and 13) and their inhibitors (TIMP1 and 2) were quantified in both cell types; only the production of proMMP2 was increased in cells derived from patients with varicose veins. Conclusions - These findings suggest that the synthesis of collagen I and III is dysregulated in dermal fibroblasts derived from patients with varicose veins. These results are comparable with those observed in smooth muscle cells derived from varicose veins, thus suggesting a systemic alteration of tissue remodeling in subjects with varicose veins.
Persistent Identifierhttp://hdl.handle.net/10722/171286
ISSN
2015 Impact Factor: 17.047
2015 SCImago Journal Rankings: 7.853
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSansilvestriMorel, Pen_US
dc.contributor.authorRupin, Aen_US
dc.contributor.authorJaisson, Sen_US
dc.contributor.authorFabiani, JNen_US
dc.contributor.authorVerbeuren, TJen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:13:11Z-
dc.date.available2012-10-30T06:13:11Z-
dc.date.issued2002en_US
dc.identifier.citationCirculation, 2002, v. 106 n. 4, p. 479-483en_US
dc.identifier.issn0009-7322en_US
dc.identifier.urihttp://hdl.handle.net/10722/171286-
dc.description.abstractBackground - The dilatation and tortuosity observed in varicose veins provide evidence for progressive venous wall remodeling associated with abnormalities of smooth muscle cells and extracellular matrix. The present study was designed to examine if the phenotypic modulations observed in the venous smooth muscle cells of patients with varicose veins were also present in their dermal fibroblasts. Methods and Results - Collagen type I (collagen I), type III (collagen III), and type V (collagen V) were compared in dermal fibroblasts derived from the skin of control subjects and patients with varicose veins. The synthesis of collagen I, the release of its metabolites, and the expression of its mRNA were increased in fibroblasts from patients with varicose veins, whereas the synthesis of collagen III was decreased but not correlated with a decrease in mRNA expression and in metabolite release. Matrix metalloproteinases (MMP1, 2, 7, 8, 9, and 13) and their inhibitors (TIMP1 and 2) were quantified in both cell types; only the production of proMMP2 was increased in cells derived from patients with varicose veins. Conclusions - These findings suggest that the synthesis of collagen I and III is dysregulated in dermal fibroblasts derived from patients with varicose veins. These results are comparable with those observed in smooth muscle cells derived from varicose veins, thus suggesting a systemic alteration of tissue remodeling in subjects with varicose veins.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.orgen_US
dc.relation.ispartofCirculationen_US
dc.subject.meshAgeden_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCollagen Type I - Biosynthesis - Genetics - Metabolismen_US
dc.subject.meshCollagen Type Iii - Biosynthesis - Genetics - Metabolismen_US
dc.subject.meshCulture Media - Analysisen_US
dc.subject.meshFemaleen_US
dc.subject.meshFibrillar Collagens - Biosynthesisen_US
dc.subject.meshFibroblasts - Metabolismen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHumansen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMatrix Metalloproteinases - Biosynthesisen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMuscle, Smooth, Vascular - Metabolismen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshSkin - Cytology - Metabolismen_US
dc.subject.meshTissue Inhibitor Of Metalloproteinases - Biosynthesisen_US
dc.subject.meshVaricose Veins - Genetics - Metabolism - Pathologyen_US
dc.subject.meshVeins - Cytology - Metabolismen_US
dc.titleSynthesis of collagen is dysregulated in cultured fibroblasts derived from skin of subjects with varicose veins as it is in venous smooth muscle cellsen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1161/01.CIR.0000022846.22923.46en_US
dc.identifier.pmid12135949-
dc.identifier.scopuseid_2-s2.0-0037162375en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037162375&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume106en_US
dc.identifier.issue4en_US
dc.identifier.spage479en_US
dc.identifier.epage483en_US
dc.identifier.isiWOS:000177154000017-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSansilvestriMorel, P=7801596301en_US
dc.identifier.scopusauthoridRupin, A=6701753601en_US
dc.identifier.scopusauthoridJaisson, S=22934066300en_US
dc.identifier.scopusauthoridFabiani, JN=7102990953en_US
dc.identifier.scopusauthoridVerbeuren, TJ=7007006534en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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