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Article: Consequences of reduced production of NO on vascular reactivity of porcine coronary arteries after angioplasty: Importance of EDHF

TitleConsequences of reduced production of NO on vascular reactivity of porcine coronary arteries after angioplasty: Importance of EDHF
Authors
KeywordsBradykinin
Cyclic Nucleotides
Endothelium-Derived Hyperpolarizing Factor
Nitric Oxide
Regenerated Endothelium
Issue Date2002
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 2002, v. 136 n. 8, p. 1153-1161 How to Cite?
Abstract1. The consequences of the reduced production of nitric oxide (NO) by cells from regenerated endothelium were investigated by measuring membrane potential of smooth muscle cells (SMCs), isometric tension and cyclic nucleotides content in porcine coronary arteries with intimal thickening, four weeks following angioplasty. 2. Under basal conditions, SMCs of coronary arteries with regenerated endothelium were depolarized by 10 mV. This depolarization was associated with 82% decreased level of cGMP without alteration in cAMP. 3. Sodium nitroprusside (SNP, 1 μM) repolarized SMCs of the previously denuded coronary arteries. This repolarization was abolished by 1H-[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 10 μM) and not suppressed by glibenclamide (10 μM), iberiotoxin (IbTX, 100 nM) and the combination of charybdotoxin (ChTX, 40 nM) plus apamin (100 nM). 4. Four-aminopyridine (4-AP, 1-5 mM) generated spontaneous rhythmic activities only in coronary arteries with regenerated endothelium which were abolished by SNP. Nevertheless, 4-AP did not suppress the repolarization induced by SNP. 5. In vascular segments with regenerated endothelium, contracted with prostaglandin F 2α (PGF 2α), relaxation to bradykinin (BK, 30 nM) was unaltered despite a reduced production of cGMP (-70%). Indomethacin (10 μM) plus N ω-nitro-L-arginine (L-NA, 30 μM) reduced relaxation (-12% and -35% for native and regenerated endothelium, respectively) but did not abolish it. 6. The hyperpolarizations induced by BK were not altered by the presence of indomethacin and L-NA and were unchanged in segments with regenerated endothelium. 7. These data are consistent with a contribution of impairment in NO production to the depolarization of SMCs. Nevertheless, EDHF responses to BK are sufficient to maintain a normal relaxation after angioplasty.
Persistent Identifierhttp://hdl.handle.net/10722/171281
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorThollon, Cen_US
dc.contributor.authorFournetBourguignon, MPen_US
dc.contributor.authorSaboureau, Den_US
dc.contributor.authorLesage, Len_US
dc.contributor.authorReure, Hen_US
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorVilaine, JPen_US
dc.date.accessioned2012-10-30T06:13:09Z-
dc.date.available2012-10-30T06:13:09Z-
dc.date.issued2002en_US
dc.identifier.citationBritish Journal Of Pharmacology, 2002, v. 136 n. 8, p. 1153-1161en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/171281-
dc.description.abstract1. The consequences of the reduced production of nitric oxide (NO) by cells from regenerated endothelium were investigated by measuring membrane potential of smooth muscle cells (SMCs), isometric tension and cyclic nucleotides content in porcine coronary arteries with intimal thickening, four weeks following angioplasty. 2. Under basal conditions, SMCs of coronary arteries with regenerated endothelium were depolarized by 10 mV. This depolarization was associated with 82% decreased level of cGMP without alteration in cAMP. 3. Sodium nitroprusside (SNP, 1 μM) repolarized SMCs of the previously denuded coronary arteries. This repolarization was abolished by 1H-[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 10 μM) and not suppressed by glibenclamide (10 μM), iberiotoxin (IbTX, 100 nM) and the combination of charybdotoxin (ChTX, 40 nM) plus apamin (100 nM). 4. Four-aminopyridine (4-AP, 1-5 mM) generated spontaneous rhythmic activities only in coronary arteries with regenerated endothelium which were abolished by SNP. Nevertheless, 4-AP did not suppress the repolarization induced by SNP. 5. In vascular segments with regenerated endothelium, contracted with prostaglandin F 2α (PGF 2α), relaxation to bradykinin (BK, 30 nM) was unaltered despite a reduced production of cGMP (-70%). Indomethacin (10 μM) plus N ω-nitro-L-arginine (L-NA, 30 μM) reduced relaxation (-12% and -35% for native and regenerated endothelium, respectively) but did not abolish it. 6. The hyperpolarizations induced by BK were not altered by the presence of indomethacin and L-NA and were unchanged in segments with regenerated endothelium. 7. These data are consistent with a contribution of impairment in NO production to the depolarization of SMCs. Nevertheless, EDHF responses to BK are sufficient to maintain a normal relaxation after angioplasty.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subjectBradykininen_US
dc.subjectCyclic Nucleotidesen_US
dc.subjectEndothelium-Derived Hyperpolarizing Factoren_US
dc.subjectNitric Oxideen_US
dc.subjectRegenerated Endotheliumen_US
dc.titleConsequences of reduced production of NO on vascular reactivity of porcine coronary arteries after angioplasty: Importance of EDHFen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.bjp.0704828en_US
dc.identifier.pmid12163348-
dc.identifier.scopuseid_2-s2.0-0036687268en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036687268&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume136en_US
dc.identifier.issue8en_US
dc.identifier.spage1153en_US
dc.identifier.epage1161en_US
dc.identifier.isiWOS:000177471600012-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridThollon, C=6602540205en_US
dc.identifier.scopusauthoridFournetBourguignon, MP=6507287770en_US
dc.identifier.scopusauthoridSaboureau, D=6602795472en_US
dc.identifier.scopusauthoridLesage, L=7004179797en_US
dc.identifier.scopusauthoridReure, H=6506882220en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridVilaine, JP=7004617134en_US

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