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Article: A BKCa to Kv switch during spermatogenesis in the rat seminiferous tubules

TitleA BKCa to Kv switch during spermatogenesis in the rat seminiferous tubules
Authors
Issue Date2002
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2002, v. 67 n. 1, p. 46-54 How to Cite?
AbstractSpermatogenesis is a complex cellular event during which the diploid germ cells differentiate and divide by mitosis and meiosis at specific time points along the spermatogenic cycle to generate the haploid spermatozoa. For this complex event to go in an orderly manner, cell differentiation and division must be precisely controlled by signals arising from within and outside the seminiferous tubules. Changes in the membrane potential of the germ cells are likely to be an important part of the signaling mechanism. We have applied the whole-cell patch clamp technique to identify and characterize ion channels in different spermatogenic cells from immature and mature rat testes fractionated by discontinuous Percoll gradient. A voltage- and Ca2+-dependent, outwardly rectifying current with gating and pharmacologic properties resembling the large conductance K+ channels (BKCa) was recorded from the spermatogonia and primary spermatocytes. Another voltage-dependent, outwardly rectifying current that was sensitive to 4-aminopyridine, a Kv channel blocker, was detected in spermatocytes and early spermatids. This current is likely caused by the smaller conductance, voltage-sensitive K+ channels (Kv). In some spermatogonia, both the BKCa channels and the Kv channels could be simultaneously detected in the same cell. It appears that during the course of spermatogenesis, there is up-regulation of Kv but down-regulation of BKCa. Reverse transcription-polymerase chain reaction, Western blot analysis, and immunohistochemistry further confirmed the differential expression of the ion channels in different spermatogenic cells. We conclude that these ion channels may play an important role in the control of spermatogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/171275
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGong, XDen_US
dc.contributor.authorLi, JCHen_US
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorCheung, KHen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-10-30T06:13:06Z-
dc.date.available2012-10-30T06:13:06Z-
dc.date.issued2002en_US
dc.identifier.citationBiology Of Reproduction, 2002, v. 67 n. 1, p. 46-54en_US
dc.identifier.issn0006-3363en_US
dc.identifier.urihttp://hdl.handle.net/10722/171275-
dc.description.abstractSpermatogenesis is a complex cellular event during which the diploid germ cells differentiate and divide by mitosis and meiosis at specific time points along the spermatogenic cycle to generate the haploid spermatozoa. For this complex event to go in an orderly manner, cell differentiation and division must be precisely controlled by signals arising from within and outside the seminiferous tubules. Changes in the membrane potential of the germ cells are likely to be an important part of the signaling mechanism. We have applied the whole-cell patch clamp technique to identify and characterize ion channels in different spermatogenic cells from immature and mature rat testes fractionated by discontinuous Percoll gradient. A voltage- and Ca2+-dependent, outwardly rectifying current with gating and pharmacologic properties resembling the large conductance K+ channels (BKCa) was recorded from the spermatogonia and primary spermatocytes. Another voltage-dependent, outwardly rectifying current that was sensitive to 4-aminopyridine, a Kv channel blocker, was detected in spermatocytes and early spermatids. This current is likely caused by the smaller conductance, voltage-sensitive K+ channels (Kv). In some spermatogonia, both the BKCa channels and the Kv channels could be simultaneously detected in the same cell. It appears that during the course of spermatogenesis, there is up-regulation of Kv but down-regulation of BKCa. Reverse transcription-polymerase chain reaction, Western blot analysis, and immunohistochemistry further confirmed the differential expression of the ion channels in different spermatogenic cells. We conclude that these ion channels may play an important role in the control of spermatogenesis.en_US
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_US
dc.relation.ispartofBiology of Reproductionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Membrane - Metabolismen_US
dc.subject.meshCentrifugationen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshKv1.1 Potassium Channelen_US
dc.subject.meshKv1.3 Potassium Channelen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentials - Physiologyen_US
dc.subject.meshPatch-Clamp Techniquesen_US
dc.subject.meshPotassium Channels - Genetics - Physiologyen_US
dc.subject.meshPotassium Channels, Voltage-Gateden_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSeminiferous Tubules - Physiologyen_US
dc.subject.meshSpermatids - Metabolismen_US
dc.subject.meshSpermatocytes - Metabolismen_US
dc.subject.meshSpermatogenesis - Physiologyen_US
dc.subject.meshSpermatogonia - Metabolismen_US
dc.titleA BKCa to Kv switch during spermatogenesis in the rat seminiferous tubulesen_US
dc.typeArticleen_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.emailCheung, KH:kingho.cheung@hku.hken_US
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.identifier.authorityCheung, KH=rp01463en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1095/biolreprod67.1.46-
dc.identifier.pmid12079998-
dc.identifier.scopuseid_2-s2.0-0036086463en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036086463&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume67en_US
dc.identifier.issue1en_US
dc.identifier.spage46en_US
dc.identifier.epage54en_US
dc.identifier.isiWOS:000176544100007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGong, XD=7201999007en_US
dc.identifier.scopusauthoridLi, JCH=7410060637en_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US
dc.identifier.scopusauthoridCheung, KH=14007487800en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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