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Article: Na+ reabsorption in cultured rat epididymal epithelium via the Na+/nucleoside cotransporter

TitleNa+ reabsorption in cultured rat epididymal epithelium via the Na+/nucleoside cotransporter
Authors
Issue Date2001
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2001, v. 64 n. 3, p. 764-769 How to Cite?
AbstractThe effect of nucleoside on Na+ reabsorption via Na+/nucleoside cotransporter in cultured rat epididymal epithelia was studied by short-circuit current (Isc) technique. Guanosine added apically stimulated Isc in a dose-dependent manner, with a median effective concentration (EC50) of 7 ± 2 μM (mean ± SEM). Removal of Na+ from the apical bathing solution or pretreatment with a nonspecific Na+/nucleoside cotransporter inhibitor, phloridzin, completely blocked the Isc response to guanosine. Moreover, the guanosine response was abolished by pretreatment of the tissue with ouabain, a Na+/K+-ATPase inhibitor, suggesting the involvement of Na+/nucleoside cotransporter on the apical side and Na+/K+-ATPase on the basolateral side in Na+ reabsorption. In contrast, the Isc response to guanosine was not affected after desensitization of purinoceptors by ATP. Addition of the Na+/K+/2Cl- symport inhibitor bumetanide to the basolateral side or the nonspecific Cl- channel blocker diphenylamine-2-carboxylate to the apical side showed no effect on the Isc response to guanosine, excluding stimulation of Cl- secretion by guanosine as the cause of the guanosine-induced Isc. The Isc response to purine nucleoside (guanosine and inosine) was much higher than that to pyrimidine nucleoside (thymidine and cytidine). Consistent with substrate specificity, results of reverse transcription-polymerase chain reaction revealed mRNA for concentrative nucleoside transporter (CNT2), which is a purine nucleoside-selective Na+/nucleoside cotransporter in the epididymis, but not for CNT1. It is suggested that the Na+/nucleoside cotransporter (i.e., CNT2) may be one of the elements involved in Na+ and fluid reabsorption in the epididymis, thereby providing an optimal microenvironment for the maturation and storage of spermatozoa.
Persistent Identifierhttp://hdl.handle.net/10722/171267
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorCheung, KHen_US
dc.contributor.authorTse, CMen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-10-30T06:13:03Z-
dc.date.available2012-10-30T06:13:03Z-
dc.date.issued2001en_US
dc.identifier.citationBiology Of Reproduction, 2001, v. 64 n. 3, p. 764-769en_US
dc.identifier.issn0006-3363en_US
dc.identifier.urihttp://hdl.handle.net/10722/171267-
dc.description.abstractThe effect of nucleoside on Na+ reabsorption via Na+/nucleoside cotransporter in cultured rat epididymal epithelia was studied by short-circuit current (Isc) technique. Guanosine added apically stimulated Isc in a dose-dependent manner, with a median effective concentration (EC50) of 7 ± 2 μM (mean ± SEM). Removal of Na+ from the apical bathing solution or pretreatment with a nonspecific Na+/nucleoside cotransporter inhibitor, phloridzin, completely blocked the Isc response to guanosine. Moreover, the guanosine response was abolished by pretreatment of the tissue with ouabain, a Na+/K+-ATPase inhibitor, suggesting the involvement of Na+/nucleoside cotransporter on the apical side and Na+/K+-ATPase on the basolateral side in Na+ reabsorption. In contrast, the Isc response to guanosine was not affected after desensitization of purinoceptors by ATP. Addition of the Na+/K+/2Cl- symport inhibitor bumetanide to the basolateral side or the nonspecific Cl- channel blocker diphenylamine-2-carboxylate to the apical side showed no effect on the Isc response to guanosine, excluding stimulation of Cl- secretion by guanosine as the cause of the guanosine-induced Isc. The Isc response to purine nucleoside (guanosine and inosine) was much higher than that to pyrimidine nucleoside (thymidine and cytidine). Consistent with substrate specificity, results of reverse transcription-polymerase chain reaction revealed mRNA for concentrative nucleoside transporter (CNT2), which is a purine nucleoside-selective Na+/nucleoside cotransporter in the epididymis, but not for CNT1. It is suggested that the Na+/nucleoside cotransporter (i.e., CNT2) may be one of the elements involved in Na+ and fluid reabsorption in the epididymis, thereby providing an optimal microenvironment for the maturation and storage of spermatozoa.en_US
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_US
dc.relation.ispartofBiology of Reproductionen_US
dc.subject.meshAbsorptionen_US
dc.subject.meshAdenosine Triphosphate - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnthranilic Acids - Pharmacologyen_US
dc.subject.meshBumetanide - Pharmacologyen_US
dc.subject.meshCalcium Channel Blockers - Pharmacologyen_US
dc.subject.meshCarrier Proteins - Biosynthesis - Genetics - Metabolismen_US
dc.subject.meshChloride Channels - Antagonists & Inhibitors - Physiologyen_US
dc.subject.meshDna - Chemistry - Genetics - Metabolismen_US
dc.subject.meshEpididymis - Metabolismen_US
dc.subject.meshEpithelial Cells - Metabolismen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGuanosine - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Transport Proteinsen_US
dc.subject.meshPatch-Clamp Techniquesen_US
dc.subject.meshPhlorhizin - Pharmacologyen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSodium - Metabolismen_US
dc.subject.meshSodium-Potassium-Exchanging Atpase - Antagonists & Inhibitors - Metabolismen_US
dc.subject.meshSubstrate Specificityen_US
dc.titleNa+ reabsorption in cultured rat epididymal epithelium via the Na+/nucleoside cotransporteren_US
dc.typeArticleen_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.emailCheung, KH:kingho.cheung@hku.hken_US
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.identifier.authorityCheung, KH=rp01463en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1095/biolreprod64.3.764-
dc.identifier.pmid11207189-
dc.identifier.scopuseid_2-s2.0-0035113314en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035113314&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume64en_US
dc.identifier.issue3en_US
dc.identifier.spage764en_US
dc.identifier.epage769en_US
dc.identifier.isiWOS:000167120400004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US
dc.identifier.scopusauthoridCheung, KH=14007487800en_US
dc.identifier.scopusauthoridTse, CM=7103295076en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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