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Article: Characterization of nucleoside transport systems in cultured rat epididymal epithelium

TitleCharacterization of nucleoside transport systems in cultured rat epididymal epithelium
Authors
Issue Date2001
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 2001, v. 280 n. 5 49-5, p. C1076-C1082 How to Cite?
AbstractThe nucleoside transport systems in cultured epididymal epithelium were characterized and found to be similar between the proximal (caput and corpus) and distal (cauda) regions of the epididymis. Functional studies revealed that 70% of the total nucleoside uptake was Na+ dependent, while 30% was Na+ independent. The Na+-independent nucleoside transport was mediated by both the equilibrative nitrobenzylthioinosine (NBMPR)-sensitive system (40%) and the NBMPR-insensitive system (60%), which was supported by a biphasic dose response to NBMPR inhibition. The Na+-dependent [3H]uridine uptake was selectively inhibited 80% by purine nucleosides, indicating that the purine nucleoside-selective N1 system is predominant. Since Na+-dependent [3H]guanosine uptake was inhibited by thymidine by 20% and Na+-dependent [3H]thymidine uptake was broadly inhibited by purine and pyrimidine nucleosides, this suggested the presence of the broadly selective N3 system accounting for 20% of Na+-dependent nucleoside uptake. Results of RT-PCR confirmed the presence of mRNA for equilibrative nucleoside transporter (ENT) 1, ENT2, and concentrative nucleoside transporter (CNT) 2 and the absence of CNT1. It is suggested that the nucleoside transporters in epididymis may be important for sperm maturation by regulating the extracellular concentration of adenosine in epididymal plasma.
Persistent Identifierhttp://hdl.handle.net/10722/171266
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorWard, JLen_US
dc.contributor.authorWong, PYDen_US
dc.contributor.authorTse, CMen_US
dc.date.accessioned2012-10-30T06:13:03Z-
dc.date.available2012-10-30T06:13:03Z-
dc.date.issued2001en_US
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 2001, v. 280 n. 5 49-5, p. C1076-C1082en_US
dc.identifier.issn0363-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/171266-
dc.description.abstractThe nucleoside transport systems in cultured epididymal epithelium were characterized and found to be similar between the proximal (caput and corpus) and distal (cauda) regions of the epididymis. Functional studies revealed that 70% of the total nucleoside uptake was Na+ dependent, while 30% was Na+ independent. The Na+-independent nucleoside transport was mediated by both the equilibrative nitrobenzylthioinosine (NBMPR)-sensitive system (40%) and the NBMPR-insensitive system (60%), which was supported by a biphasic dose response to NBMPR inhibition. The Na+-dependent [3H]uridine uptake was selectively inhibited 80% by purine nucleosides, indicating that the purine nucleoside-selective N1 system is predominant. Since Na+-dependent [3H]guanosine uptake was inhibited by thymidine by 20% and Na+-dependent [3H]thymidine uptake was broadly inhibited by purine and pyrimidine nucleosides, this suggested the presence of the broadly selective N3 system accounting for 20% of Na+-dependent nucleoside uptake. Results of RT-PCR confirmed the presence of mRNA for equilibrative nucleoside transporter (ENT) 1, ENT2, and concentrative nucleoside transporter (CNT) 2 and the absence of CNT1. It is suggested that the nucleoside transporters in epididymis may be important for sperm maturation by regulating the extracellular concentration of adenosine in epididymal plasma.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Transport - Drug Effectsen_US
dc.subject.meshCarrier Proteins - Genetics - Metabolismen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpididymis - Cytology - Physiologyen_US
dc.subject.meshEpithelial Cells - Cytology - Physiologyen_US
dc.subject.meshEquilibrative Nucleoside Transporter 1en_US
dc.subject.meshEquilibrative-Nucleoside Transporter 2en_US
dc.subject.meshGuanosine - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Genetics - Metabolismen_US
dc.subject.meshMembrane Transport Proteinsen_US
dc.subject.meshNucleosides - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSodium - Physiologyen_US
dc.subject.meshThioinosine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshThymidine - Metabolismen_US
dc.subject.meshTritiumen_US
dc.subject.meshUridine - Metabolismen_US
dc.titleCharacterization of nucleoside transport systems in cultured rat epididymal epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11287319-
dc.identifier.scopuseid_2-s2.0-0035041002en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035041002&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume280en_US
dc.identifier.issue5 49-5en_US
dc.identifier.spageC1076en_US
dc.identifier.epageC1082en_US
dc.identifier.isiWOS:000168221100006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US
dc.identifier.scopusauthoridWard, JL=7404119073en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US
dc.identifier.scopusauthoridTse, CM=7103295076en_US

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