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- Scopus: eid_2-s2.0-0035041002
- PMID: 11287319
- WOS: WOS:000168221100006
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Article: Characterization of nucleoside transport systems in cultured rat epididymal epithelium
Title | Characterization of nucleoside transport systems in cultured rat epididymal epithelium |
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Authors | |
Keywords | Sperm maturation |
Issue Date | 2001 |
Publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/ |
Citation | American Journal Of Physiology - Cell Physiology, 2001, v. 280 n. 5 49-5, p. C1076-C1082 How to Cite? |
Abstract | The nucleoside transport systems in cultured epididymal epithelium were characterized and found to be similar between the proximal (caput and corpus) and distal (cauda) regions of the epididymis. Functional studies revealed that 70% of the total nucleoside uptake was Na+ dependent, while 30% was Na+ independent. The Na+-independent nucleoside transport was mediated by both the equilibrative nitrobenzylthioinosine (NBMPR)-sensitive system (40%) and the NBMPR-insensitive system (60%), which was supported by a biphasic dose response to NBMPR inhibition. The Na+-dependent [3H]uridine uptake was selectively inhibited 80% by purine nucleosides, indicating that the purine nucleoside-selective N1 system is predominant. Since Na+-dependent [3H]guanosine uptake was inhibited by thymidine by 20% and Na+-dependent [3H]thymidine uptake was broadly inhibited by purine and pyrimidine nucleosides, this suggested the presence of the broadly selective N3 system accounting for 20% of Na+-dependent nucleoside uptake. Results of RT-PCR confirmed the presence of mRNA for equilibrative nucleoside transporter (ENT) 1, ENT2, and concentrative nucleoside transporter (CNT) 2 and the absence of CNT1. It is suggested that the nucleoside transporters in epididymis may be important for sperm maturation by regulating the extracellular concentration of adenosine in epididymal plasma. |
Persistent Identifier | http://hdl.handle.net/10722/171266 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.711 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leung, GPH | en_US |
dc.contributor.author | Ward, JL | en_US |
dc.contributor.author | Wong, PYD | en_US |
dc.contributor.author | Tse, CM | en_US |
dc.date.accessioned | 2012-10-30T06:13:03Z | - |
dc.date.available | 2012-10-30T06:13:03Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | American Journal Of Physiology - Cell Physiology, 2001, v. 280 n. 5 49-5, p. C1076-C1082 | en_US |
dc.identifier.issn | 0363-6143 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171266 | - |
dc.description.abstract | The nucleoside transport systems in cultured epididymal epithelium were characterized and found to be similar between the proximal (caput and corpus) and distal (cauda) regions of the epididymis. Functional studies revealed that 70% of the total nucleoside uptake was Na+ dependent, while 30% was Na+ independent. The Na+-independent nucleoside transport was mediated by both the equilibrative nitrobenzylthioinosine (NBMPR)-sensitive system (40%) and the NBMPR-insensitive system (60%), which was supported by a biphasic dose response to NBMPR inhibition. The Na+-dependent [3H]uridine uptake was selectively inhibited 80% by purine nucleosides, indicating that the purine nucleoside-selective N1 system is predominant. Since Na+-dependent [3H]guanosine uptake was inhibited by thymidine by 20% and Na+-dependent [3H]thymidine uptake was broadly inhibited by purine and pyrimidine nucleosides, this suggested the presence of the broadly selective N3 system accounting for 20% of Na+-dependent nucleoside uptake. Results of RT-PCR confirmed the presence of mRNA for equilibrative nucleoside transporter (ENT) 1, ENT2, and concentrative nucleoside transporter (CNT) 2 and the absence of CNT1. It is suggested that the nucleoside transporters in epididymis may be important for sperm maturation by regulating the extracellular concentration of adenosine in epididymal plasma. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Cell Physiology | en_US |
dc.subject | Sperm maturation | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Biological Transport - Drug Effects | en_US |
dc.subject.mesh | Carrier Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Epididymis - Cytology - Physiology | en_US |
dc.subject.mesh | Epithelial Cells - Cytology - Physiology | en_US |
dc.subject.mesh | Equilibrative Nucleoside Transporter 1 | en_US |
dc.subject.mesh | Equilibrative-Nucleoside Transporter 2 | en_US |
dc.subject.mesh | Guanosine - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Membrane Transport Proteins | en_US |
dc.subject.mesh | Nucleosides - Metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Sodium - Physiology | en_US |
dc.subject.mesh | Thioinosine - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Thymidine - Metabolism | en_US |
dc.subject.mesh | Tritium | en_US |
dc.subject.mesh | Uridine - Metabolism | en_US |
dc.title | Characterization of nucleoside transport systems in cultured rat epididymal epithelium | en_US |
dc.type | Article | en_US |
dc.identifier.email | Leung, GPH:gphleung@hkucc.hku.hk | en_US |
dc.identifier.authority | Leung, GPH=rp00234 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 11287319 | - |
dc.identifier.scopus | eid_2-s2.0-0035041002 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0035041002&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 280 | en_US |
dc.identifier.issue | 5 49-5 | en_US |
dc.identifier.spage | C1076 | en_US |
dc.identifier.epage | C1082 | en_US |
dc.identifier.isi | WOS:000168221100006 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Leung, GPH=35963668200 | en_US |
dc.identifier.scopusauthorid | Ward, JL=7404119073 | en_US |
dc.identifier.scopusauthorid | Wong, PYD=7403980262 | en_US |
dc.identifier.scopusauthorid | Tse, CM=7103295076 | en_US |
dc.identifier.issnl | 0363-6143 | - |