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Article: Expression of cystic fibrosis transmembrane conductance regulator in rat efferent duct epithelium

TitleExpression of cystic fibrosis transmembrane conductance regulator in rat efferent duct epithelium
Authors
Issue Date2001
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2001, v. 64 n. 5, p. 1509-1515 How to Cite?
AbstractThe expression of cystic fibrosis transmembrane conductance regulator (CFTR) was studied in rat efferent ducts. Under wholecell patch-clamp condition, efferent duct cells responded to intracellular cAMP with a rise in inward current. The cAMP-activated current exhibited a linear I-V relationship and time- and voltage-independent characteristics. The current was inhibited by the Cl- channel blocker diphenylamine 2,2′-dicarboxylic acid (DPC) in a voltage-dependent manner and reversed at 24 ± 0.5 mV, close to the equilibrium potential for Cl- (30 mV), suggesting that the current was Cl- selective. The cAMP-activated current displayed a permeability sequence of Br- > Cl- > I-. Short-circuit current measurement in cultured rat efferent duct epithelia also revealed a cAMP-activated inward current inhibitable by DPC. These electrophysiological properties of the cAMP-activated Cl- conductance in the efferent duct were consistent with those reported for CFTR. In support of the functional studies, reverse transcription polymerase chain reaction revealed the presence of CFTR message in cultured efferent duct epithelium. Immunohistochemical studies in intact rats also demonstrated CFTR protein at the apical membrane of the principal cells of efferent duct. CFTR may play a role in modulating fluid transport in the efferent duct.
Persistent Identifierhttp://hdl.handle.net/10722/171265
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorGong, XDen_US
dc.contributor.authorCheung, KHen_US
dc.contributor.authorChengChew, SBen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-10-30T06:13:03Z-
dc.date.available2012-10-30T06:13:03Z-
dc.date.issued2001en_US
dc.identifier.citationBiology Of Reproduction, 2001, v. 64 n. 5, p. 1509-1515en_US
dc.identifier.issn0006-3363en_US
dc.identifier.urihttp://hdl.handle.net/10722/171265-
dc.description.abstractThe expression of cystic fibrosis transmembrane conductance regulator (CFTR) was studied in rat efferent ducts. Under wholecell patch-clamp condition, efferent duct cells responded to intracellular cAMP with a rise in inward current. The cAMP-activated current exhibited a linear I-V relationship and time- and voltage-independent characteristics. The current was inhibited by the Cl- channel blocker diphenylamine 2,2′-dicarboxylic acid (DPC) in a voltage-dependent manner and reversed at 24 ± 0.5 mV, close to the equilibrium potential for Cl- (30 mV), suggesting that the current was Cl- selective. The cAMP-activated current displayed a permeability sequence of Br- > Cl- > I-. Short-circuit current measurement in cultured rat efferent duct epithelia also revealed a cAMP-activated inward current inhibitable by DPC. These electrophysiological properties of the cAMP-activated Cl- conductance in the efferent duct were consistent with those reported for CFTR. In support of the functional studies, reverse transcription polymerase chain reaction revealed the presence of CFTR message in cultured efferent duct epithelium. Immunohistochemical studies in intact rats also demonstrated CFTR protein at the apical membrane of the principal cells of efferent duct. CFTR may play a role in modulating fluid transport in the efferent duct.en_US
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_US
dc.relation.ispartofBiology of Reproductionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnionsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChloride Channels - Physiologyen_US
dc.subject.meshCyclic Amp - Pharmacologyen_US
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulator - Analysis - Genetics - Physiologyen_US
dc.subject.meshElectric Conductivityen_US
dc.subject.meshEpididymis - Chemistry - Physiologyen_US
dc.subject.meshEpithelial Cells - Chemistry - Physiologyen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshMaleen_US
dc.subject.meshPatch-Clamp Techniquesen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTestis - Chemistry - Physiologyen_US
dc.titleExpression of cystic fibrosis transmembrane conductance regulator in rat efferent duct epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.emailCheung, KH:kingho.cheung@hku.hken_US
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.identifier.authorityCheung, KH=rp01463en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11319159-
dc.identifier.scopuseid_2-s2.0-0035037658en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035037658&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume64en_US
dc.identifier.issue5en_US
dc.identifier.spage1509en_US
dc.identifier.epage1515en_US
dc.identifier.isiWOS:000168364800029-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US
dc.identifier.scopusauthoridGong, XD=7201999007en_US
dc.identifier.scopusauthoridCheung, KH=14007487800en_US
dc.identifier.scopusauthoridChengChew, SB=6701358810en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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