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- Publisher Website: 10.1095/biolreprod62.1.143
- Scopus: eid_2-s2.0-0033962314
- PMID: 10611078
- WOS: WOS:000084401500019
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Article: Activation of cystic fibrosis transmembrane conductance regulator in rat epididymal epithelium by genistein
Title | Activation of cystic fibrosis transmembrane conductance regulator in rat epididymal epithelium by genistein |
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Authors | |
Issue Date | 2000 |
Publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ |
Citation | Biology Of Reproduction, 2000, v. 62 n. 1, p. 143-149 How to Cite? |
Abstract | The effect of genistein on anion secretion via cystic fibrosis transmembrane conductance regulator (CFTR) in cultured rat cauda epididymal epithelia was studied by short-circuit current (Isc) technique. Genistein added apically stimulated a concentration-dependent rise in Isc due to Cl- and HCO3 - secretion. The genistein-induced Isc was observed in basolaterally permeabilized monolayers, suggesting that the Isc response was mediated by the apical anion channel. The response could be blocked by the nonspecific Cl- channel blocker, diphenylamine-2-carboxylate (DPC), but not by the Ca2+-activated Cl- channel blocker, 4,4'-diisothiocyanostilbene- 2,2'-disulfonic acid (DIDS). Genistein did not increase intracellular cAMP, but H-89, a protein kinase A inhibitor, completely abolished the Isc response to genistein. Moreover, pretreatment of the tissues with MDL-12330A, an adenylate cyclase inhibitor, markedly attenuated the Isc response to genistein, but the response was restored upon the addition of exogenous cAMP. Ca2+, protein kinase C, tyrosine kinase, and protein phosphatase signalling pathways were not involved in the action of genistein. It is speculated that genistein stimulates anion secretion by direct interaction with CFTR. This requires a low level of phosphorylation of CFTR by basal protein kinase A activity. It is suggested that genistein may provide therapeutic benefit to male infertility associated with cystic fibrosis. |
Persistent Identifier | http://hdl.handle.net/10722/171237 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.022 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leung, GPH | en_US |
dc.contributor.author | Wong, PYD | en_US |
dc.date.accessioned | 2012-10-30T06:12:52Z | - |
dc.date.available | 2012-10-30T06:12:52Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | Biology Of Reproduction, 2000, v. 62 n. 1, p. 143-149 | en_US |
dc.identifier.issn | 0006-3363 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171237 | - |
dc.description.abstract | The effect of genistein on anion secretion via cystic fibrosis transmembrane conductance regulator (CFTR) in cultured rat cauda epididymal epithelia was studied by short-circuit current (Isc) technique. Genistein added apically stimulated a concentration-dependent rise in Isc due to Cl- and HCO3 - secretion. The genistein-induced Isc was observed in basolaterally permeabilized monolayers, suggesting that the Isc response was mediated by the apical anion channel. The response could be blocked by the nonspecific Cl- channel blocker, diphenylamine-2-carboxylate (DPC), but not by the Ca2+-activated Cl- channel blocker, 4,4'-diisothiocyanostilbene- 2,2'-disulfonic acid (DIDS). Genistein did not increase intracellular cAMP, but H-89, a protein kinase A inhibitor, completely abolished the Isc response to genistein. Moreover, pretreatment of the tissues with MDL-12330A, an adenylate cyclase inhibitor, markedly attenuated the Isc response to genistein, but the response was restored upon the addition of exogenous cAMP. Ca2+, protein kinase C, tyrosine kinase, and protein phosphatase signalling pathways were not involved in the action of genistein. It is speculated that genistein stimulates anion secretion by direct interaction with CFTR. This requires a low level of phosphorylation of CFTR by basal protein kinase A activity. It is suggested that genistein may provide therapeutic benefit to male infertility associated with cystic fibrosis. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ | en_US |
dc.relation.ispartof | Biology of Reproduction | en_US |
dc.subject.mesh | 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - Pharmacology | en_US |
dc.subject.mesh | Adenylate Cyclase - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Anthranilic Acids - Pharmacology | en_US |
dc.subject.mesh | Bicarbonates - Metabolism | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Chloride Channels - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Chlorides - Metabolism | en_US |
dc.subject.mesh | Cyclic Amp - Metabolism | en_US |
dc.subject.mesh | Cystic Fibrosis Transmembrane Conductance Regulator - Metabolism | en_US |
dc.subject.mesh | Electric Conductivity | en_US |
dc.subject.mesh | Enzyme Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Epididymis - Metabolism | en_US |
dc.subject.mesh | Epithelium - Metabolism | en_US |
dc.subject.mesh | Genistein - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Protein Kinase Inhibitors | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Thapsigargin - Pharmacology | en_US |
dc.title | Activation of cystic fibrosis transmembrane conductance regulator in rat epididymal epithelium by genistein | en_US |
dc.type | Article | en_US |
dc.identifier.email | Leung, GPH:gphleung@hkucc.hku.hk | en_US |
dc.identifier.authority | Leung, GPH=rp00234 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1095/biolreprod62.1.143 | - |
dc.identifier.pmid | 10611078 | - |
dc.identifier.scopus | eid_2-s2.0-0033962314 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033962314&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 62 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 143 | en_US |
dc.identifier.epage | 149 | en_US |
dc.identifier.isi | WOS:000084401500019 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Leung, GPH=35963668200 | en_US |
dc.identifier.scopusauthorid | Wong, PYD=7403980262 | en_US |
dc.identifier.issnl | 0006-3363 | - |