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Article: Potassium ions and endothelium-derived hyperpolarizing factor in guinea-pig carotid and porcine coronary arteries

TitlePotassium ions and endothelium-derived hyperpolarizing factor in guinea-pig carotid and porcine coronary arteries
Authors
Issue Date1999
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 1999, v. 127 n. 1, p. 27-34 How to Cite?
Abstract1. Experiments were designed to determine in two arteries (the guinea-pig carotid and the porcine coronary arteries) whether or not the endothelium-derived hyperpolarizing factor (EDHF) can be identified as potassium ions, and to determine whether or not the inwardly rectifying potassium current and the Na+/K+ pump are involved in the hyperpolarization mediated by EDHF. 2. The membrane potential of vascular smooth muscle cells was recorded with intracellular microelectrodes in the presence of N(ω)-L-nitro-arginine (L-NA) and indomethacin. 3. In vascular smooth muscle cells of guinea-pig carotid and porcine coronary arteries, acetylcholine and bradykinin induced endothelium-dependent hyperpolarizations (-18 ± 1 mV, n = 39 and -19 ± 1 mV, n = 7, respectively). The hyperpolarizations were not affected significantly by ouabain (1 μM), barium chloride (up to 100 μM) or the combination of ouabain plus barium. 4. In both arteries, increasing extracellular potassium concentration by 5 or 10 mM induced either depolarization or in a very few cases small hyperpolarizations which never exceeded 2 mV. 5. In isolated smooth muscle cells of the guinea-pig carotid artery, patch-clamp experiments shows that only 20% of the vascular smooth muscle cells expressed inwardly rectifying potassium channels. The current density recorded was low (0.5 ± 0.1 pA pF-1, n = 8). 6. These results indicate that, in two different vascular preparations, barium sensitive-inwardly rectifying potassium conductance and the ouabain sensitive-Na+/K+ pump are not involved in the EDHF-mediated hyperpolarization. Furthermore, potassium did not mimic the effect of EDHF pointing out that potassium and EDHF are not the same entity in those arteries.
Persistent Identifierhttp://hdl.handle.net/10722/171224
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQuignard, JFen_US
dc.contributor.authorFélétou, Men_US
dc.contributor.authorThollon, Cen_US
dc.contributor.authorVilaine, JPen_US
dc.contributor.authorDuhault, Jen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:48Z-
dc.date.available2012-10-30T06:12:48Z-
dc.date.issued1999en_US
dc.identifier.citationBritish Journal Of Pharmacology, 1999, v. 127 n. 1, p. 27-34en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/171224-
dc.description.abstract1. Experiments were designed to determine in two arteries (the guinea-pig carotid and the porcine coronary arteries) whether or not the endothelium-derived hyperpolarizing factor (EDHF) can be identified as potassium ions, and to determine whether or not the inwardly rectifying potassium current and the Na+/K+ pump are involved in the hyperpolarization mediated by EDHF. 2. The membrane potential of vascular smooth muscle cells was recorded with intracellular microelectrodes in the presence of N(ω)-L-nitro-arginine (L-NA) and indomethacin. 3. In vascular smooth muscle cells of guinea-pig carotid and porcine coronary arteries, acetylcholine and bradykinin induced endothelium-dependent hyperpolarizations (-18 ± 1 mV, n = 39 and -19 ± 1 mV, n = 7, respectively). The hyperpolarizations were not affected significantly by ouabain (1 μM), barium chloride (up to 100 μM) or the combination of ouabain plus barium. 4. In both arteries, increasing extracellular potassium concentration by 5 or 10 mM induced either depolarization or in a very few cases small hyperpolarizations which never exceeded 2 mV. 5. In isolated smooth muscle cells of the guinea-pig carotid artery, patch-clamp experiments shows that only 20% of the vascular smooth muscle cells expressed inwardly rectifying potassium channels. The current density recorded was low (0.5 ± 0.1 pA pF-1, n = 8). 6. These results indicate that, in two different vascular preparations, barium sensitive-inwardly rectifying potassium conductance and the ouabain sensitive-Na+/K+ pump are not involved in the EDHF-mediated hyperpolarization. Furthermore, potassium did not mimic the effect of EDHF pointing out that potassium and EDHF are not the same entity in those arteries.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Factors - Metabolismen_US
dc.subject.meshCarotid Arteries - Metabolismen_US
dc.subject.meshCoronary Vessels - Metabolismen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentialsen_US
dc.subject.meshMicroelectrodesen_US
dc.subject.meshPatch-Clamp Techniquesen_US
dc.subject.meshPotassium - Metabolism - Pharmacologyen_US
dc.subject.meshPotassium Channels - Drug Effects - Metabolismen_US
dc.subject.meshSwineen_US
dc.titlePotassium ions and endothelium-derived hyperpolarizing factor in guinea-pig carotid and porcine coronary arteriesen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.bjp.0702493en_US
dc.identifier.pmid10369452-
dc.identifier.scopuseid_2-s2.0-0032938529en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032938529&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume127en_US
dc.identifier.issue1en_US
dc.identifier.spage27en_US
dc.identifier.epage34en_US
dc.identifier.isiWOS:000080097400004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridQuignard, JF=13606215800en_US
dc.identifier.scopusauthoridFélétou, M=7006461826en_US
dc.identifier.scopusauthoridThollon, C=6602540205en_US
dc.identifier.scopusauthoridVilaine, JP=7004617134en_US
dc.identifier.scopusauthoridDuhault, J=7005108808en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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