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Article: Inhibitors of the cytochrome P450-mono-oxygenase and endothelium-dependent hyperpolarizations in the guinea-pig isolated carotid artery

TitleInhibitors of the cytochrome P450-mono-oxygenase and endothelium-dependent hyperpolarizations in the guinea-pig isolated carotid artery
Authors
Issue Date1996
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 1996, v. 117 n. 4, p. 607-610 How to Cite?
Abstract1 Transmembrane potentials were recorded from isolated carotid arteries of the guinea-pig superfused with modified Krebs-Ringer bicarbonate solution. Smooth muscle cells were impaled with sharp intracellular microelectrodes. 2 Acetylcholine (1 μM) induced an endothelium-dependent hyperpolarization (14.3 ± 2.8 mV, n = 6) which was not affected (15.1 ± 1.1 mV, n = 35) by inhibitors of cyclo-oxygenase (indomethacin, 5 μM) and nitric oxide synthase (N(ω)nitro-L-arginine:L-NOARG, 100 μM). 3 The hyperpolarization produced by acetylcholine was abolished in the presence of elevated potassium (35 mM) in the superfusing physiological saline solution. 4 The acetylcholine-induced hyperpolarization was not affected by the inhibitors of cytochrome P450 mono-oxygenases, SKF525a (10 and 100 μM, 13.9 ± 2.2 and 15.3 ± 4.6 mV), metyrapone (100 μM, 13.1 ± 1.9 mV), clotrimazole (100 μM, 13.5 ± 2.7 mV), 17-octadecynoic acid (5 μM, 16.5 ± 1.9 mV), methoxsalen (10 μM, 15.3 ± 1.6 mV), the inhibitor of phospholipase A2 quinacrine (10 μM 12.8 ± 2.5 mV) and the non specific lipoxygenases/cyclo-oxygenases/cytochrome P450 inhibitor, eicosatetraynoic acid (50 μM, 15.0 ± 2.2 mV). However, the muscarinic antagonist, atropine (100 nM), abolished the hyperpolarization. 5 These results suggest that in guinea-pig carotid artery, the metabolism of arachidonic acid, either through cyclo-oxygenase, lipoxygenase or cytochrome p450 mono-oxygenase, is not involved in acetylcholine-induced endothelium-dependent hyperpolarizations.
Persistent Identifierhttp://hdl.handle.net/10722/171189
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCorriu, Cen_US
dc.contributor.authorFélétou, Men_US
dc.contributor.authorCanet, Een_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:36Z-
dc.date.available2012-10-30T06:12:36Z-
dc.date.issued1996en_US
dc.identifier.citationBritish Journal Of Pharmacology, 1996, v. 117 n. 4, p. 607-610en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/171189-
dc.description.abstract1 Transmembrane potentials were recorded from isolated carotid arteries of the guinea-pig superfused with modified Krebs-Ringer bicarbonate solution. Smooth muscle cells were impaled with sharp intracellular microelectrodes. 2 Acetylcholine (1 μM) induced an endothelium-dependent hyperpolarization (14.3 ± 2.8 mV, n = 6) which was not affected (15.1 ± 1.1 mV, n = 35) by inhibitors of cyclo-oxygenase (indomethacin, 5 μM) and nitric oxide synthase (N(ω)nitro-L-arginine:L-NOARG, 100 μM). 3 The hyperpolarization produced by acetylcholine was abolished in the presence of elevated potassium (35 mM) in the superfusing physiological saline solution. 4 The acetylcholine-induced hyperpolarization was not affected by the inhibitors of cytochrome P450 mono-oxygenases, SKF525a (10 and 100 μM, 13.9 ± 2.2 and 15.3 ± 4.6 mV), metyrapone (100 μM, 13.1 ± 1.9 mV), clotrimazole (100 μM, 13.5 ± 2.7 mV), 17-octadecynoic acid (5 μM, 16.5 ± 1.9 mV), methoxsalen (10 μM, 15.3 ± 1.6 mV), the inhibitor of phospholipase A2 quinacrine (10 μM 12.8 ± 2.5 mV) and the non specific lipoxygenases/cyclo-oxygenases/cytochrome P450 inhibitor, eicosatetraynoic acid (50 μM, 15.0 ± 2.2 mV). However, the muscarinic antagonist, atropine (100 nM), abolished the hyperpolarization. 5 These results suggest that in guinea-pig carotid artery, the metabolism of arachidonic acid, either through cyclo-oxygenase, lipoxygenase or cytochrome p450 mono-oxygenase, is not involved in acetylcholine-induced endothelium-dependent hyperpolarizations.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarotid Arteries - Drug Effects - Enzymology - Physiologyen_US
dc.subject.meshCytochrome P-450 Enzyme System - Antagonists & Inhibitorsen_US
dc.subject.meshEndothelium, Vascular - Drug Effects - Enzymology - Physiologyen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentialsen_US
dc.subject.meshOxygenases - Antagonists & Inhibitorsen_US
dc.titleInhibitors of the cytochrome P450-mono-oxygenase and endothelium-dependent hyperpolarizations in the guinea-pig isolated carotid arteryen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1476-5381.1996.tb15233.x-
dc.identifier.pmid8646403-
dc.identifier.scopuseid_2-s2.0-0030068066en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030068066&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume117en_US
dc.identifier.issue4en_US
dc.identifier.spage607en_US
dc.identifier.epage610en_US
dc.identifier.isiWOS:A1996TY67700002-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridCorriu, C=6602961498en_US
dc.identifier.scopusauthoridFélétou, M=7006461826en_US
dc.identifier.scopusauthoridCanet, E=7006072145en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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