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Article: Stimulation of sodium pump by vasoactive intestinal peptide in guinea-pig isolated trachea: Potential contribution to mechanisms underlying relaxation of smooth muscle

TitleStimulation of sodium pump by vasoactive intestinal peptide in guinea-pig isolated trachea: Potential contribution to mechanisms underlying relaxation of smooth muscle
Authors
Issue Date1996
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 1996, v. 118 n. 3, p. 557-562 How to Cite?
Abstract1. Relaxation of airway smooth muscle induced by vasoactive intestinal peptide (VIP) is mediated by adenosine 3':5' cyclic monophosphate (cyclic AMP). An interaction between the synthesis of cyclic AMP and enzymic activity of the plasmalemmal sodium pump (Na+-K+-ATPase) exists in certain isolated cell systems. This study sought to determine the contribution of Na+-K+-ATPase activity to relaxation of airway smooth muscle evoked by VIP. 2. All experiments were performed on isolated strips of guinea-pig trachea from which the epithelium had been removed. VIP was a more potent relaxant in tissues that were contracted with carbachol than those contracted with an equi-effective depolarizing concentration of K+. 3. Ouabain (0.1 μM-10 μM) induced contraction of tracheal strips. Contraction to ouabain (5 μM) was abolished following incubation of tissues with K+-free, or Ca2+-free (+EGTA, 0.1 mM) physiological solutions. The contractile response to ouabain (5 μM) was not influenced significantly by exposure of the tissues to atropine (1 μM), phentolamine (5 μM) and diphenhydramine (1 μM) for 60 min. 4. Tissues were incubated with ouabain (5 μM; 60 min) or K+-free physiological solution (60 min) to inhibit Na+-K+-ATPase activity. These procedures reduced relaxation induced by VIP, peptide histidine isoleucine, forskolin, isoprenaline and sodium nitroprusside. 5. Relaxation to VIP was impaired significantly following exposure of tissues to a low Na+ solution (30 min) or amiloride (500 μM; 30 min). 6. Ouabain-sensitive uptake of 86Rb was measured in tracheal strips (devoid of epithelium and cartilage) as an index of Na+-K+-ATPase activity. VIP (1 μM; 2 min) caused a 4.7 fold stimulation of ouabain-sensitive uptake of 86Rb. This effect was impaired significantly by low Na+ solution. 7. The results suggest that (i) relaxation of tracheal smooth muscle to VIP is sensitive to procedures that inhibit activity of Na+-K+-ATPase and invoke a role for altered sodium pump function in the mechanisms that underlie cyclic AMP-dependent relaxation; and (ii) VIP stimulates ouabain-sensitive uptake of 86Rb in airway smooth muscle in a Na+-dependent manner.
Persistent Identifierhttp://hdl.handle.net/10722/171184
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMorrison, KJen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:34Z-
dc.date.available2012-10-30T06:12:34Z-
dc.date.issued1996en_US
dc.identifier.citationBritish Journal Of Pharmacology, 1996, v. 118 n. 3, p. 557-562en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/171184-
dc.description.abstract1. Relaxation of airway smooth muscle induced by vasoactive intestinal peptide (VIP) is mediated by adenosine 3':5' cyclic monophosphate (cyclic AMP). An interaction between the synthesis of cyclic AMP and enzymic activity of the plasmalemmal sodium pump (Na+-K+-ATPase) exists in certain isolated cell systems. This study sought to determine the contribution of Na+-K+-ATPase activity to relaxation of airway smooth muscle evoked by VIP. 2. All experiments were performed on isolated strips of guinea-pig trachea from which the epithelium had been removed. VIP was a more potent relaxant in tissues that were contracted with carbachol than those contracted with an equi-effective depolarizing concentration of K+. 3. Ouabain (0.1 μM-10 μM) induced contraction of tracheal strips. Contraction to ouabain (5 μM) was abolished following incubation of tissues with K+-free, or Ca2+-free (+EGTA, 0.1 mM) physiological solutions. The contractile response to ouabain (5 μM) was not influenced significantly by exposure of the tissues to atropine (1 μM), phentolamine (5 μM) and diphenhydramine (1 μM) for 60 min. 4. Tissues were incubated with ouabain (5 μM; 60 min) or K+-free physiological solution (60 min) to inhibit Na+-K+-ATPase activity. These procedures reduced relaxation induced by VIP, peptide histidine isoleucine, forskolin, isoprenaline and sodium nitroprusside. 5. Relaxation to VIP was impaired significantly following exposure of tissues to a low Na+ solution (30 min) or amiloride (500 μM; 30 min). 6. Ouabain-sensitive uptake of 86Rb was measured in tracheal strips (devoid of epithelium and cartilage) as an index of Na+-K+-ATPase activity. VIP (1 μM; 2 min) caused a 4.7 fold stimulation of ouabain-sensitive uptake of 86Rb. This effect was impaired significantly by low Na+ solution. 7. The results suggest that (i) relaxation of tracheal smooth muscle to VIP is sensitive to procedures that inhibit activity of Na+-K+-ATPase and invoke a role for altered sodium pump function in the mechanisms that underlie cyclic AMP-dependent relaxation; and (ii) VIP stimulates ouabain-sensitive uptake of 86Rb in airway smooth muscle in a Na+-dependent manner.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshForskolin - Pharmacologyen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Relaxation - Drug Effectsen_US
dc.subject.meshMuscle, Smooth - Drug Effectsen_US
dc.subject.meshSodium-Potassium-Exchanging Atpase - Drug Effectsen_US
dc.subject.meshTrachea - Drug Effectsen_US
dc.subject.meshVasoactive Intestinal Peptide - Pharmacologyen_US
dc.titleStimulation of sodium pump by vasoactive intestinal peptide in guinea-pig isolated trachea: Potential contribution to mechanisms underlying relaxation of smooth muscleen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1476-5381.1996.tb15438.x-
dc.identifier.pmid8762078-
dc.identifier.scopuseid_2-s2.0-0029975658en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029975658&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume118en_US
dc.identifier.issue3en_US
dc.identifier.spage557en_US
dc.identifier.epage562en_US
dc.identifier.isiWOS:A1996UM86500017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridMorrison, KJ=7102484828en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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