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Article: The effects of lidocaine and hypoxia on phospholipid biosynthesis in the isolated hamster heart

TitleThe effects of lidocaine and hypoxia on phospholipid biosynthesis in the isolated hamster heart
Authors
Issue Date1996
PublisherA O C S Press. The Journal's web site is located at http://www.aocs.org/press/lipids.htm
Citation
Lipids, 1996, v. 31 n. 10, p. 1059-1067 How to Cite?
AbstractIn this study, the effects of lidocaine and hypoxia on the biosynthesis of phospholipids in the hamster heart were examined. Hamster hearts were perfused with [1,3- 3H]glycerol under normal and hypoxic conditions, and in the absence or presence of 0.5 mg/mL lidocaine. After perfusion, the radioactivity incorporated into the various phospholipid fractions was determined. With the exception of phosphatidylcholine, the synthesis of phospholipids was generally stimulated by lidocaine perfusion. In contrast, hypoxia caused a general decrease in phospholipid biosynthesis which was partially restored by lidocaine. ATP and CTP levels were severely reduced under hypoxic conditions, but their levels were not restored by lidocaine treatment. The activities of enzymes for phospholipid synthesis were determined under the various perfusion conditions. The activity of phosphatidic acid phosphatase was elevated by lidocaine and decreased by hypoxic treatment. The activity of CTP:phosphatidic acid cytidylyltransferase was increased under hypoxia, with or without lidocaine. Despite the reduction in phosphatidylcholine biosynthesis, no change in the activity of cytidine diphosphocholine (CDPcholine):diacylglycerol cholinephosphotransferase was detected following lidocaine or hypoxic perfusion. However, enzyme activity was inhibited by the presence of lidocaine in the assay mixture. Our results indicate that the reduction in phospholipid biosynthesis under hypoxicconditions was caused mainly by diminishing high-energy nucleotide levels. The enhancement of phospholipid biosynthesis by lidocaine appeared to be mediated in part by modulation of enzyme activities.
Persistent Identifierhttp://hdl.handle.net/10722/171178
ISSN
2015 Impact Factor: 1.892
2015 SCImago Journal Rankings: 0.795
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, JTen_US
dc.contributor.authorMan, RYKen_US
dc.contributor.authorChoy, PCen_US
dc.date.accessioned2012-10-30T06:12:32Z-
dc.date.available2012-10-30T06:12:32Z-
dc.date.issued1996en_US
dc.identifier.citationLipids, 1996, v. 31 n. 10, p. 1059-1067en_US
dc.identifier.issn0024-4201en_US
dc.identifier.urihttp://hdl.handle.net/10722/171178-
dc.description.abstractIn this study, the effects of lidocaine and hypoxia on the biosynthesis of phospholipids in the hamster heart were examined. Hamster hearts were perfused with [1,3- 3H]glycerol under normal and hypoxic conditions, and in the absence or presence of 0.5 mg/mL lidocaine. After perfusion, the radioactivity incorporated into the various phospholipid fractions was determined. With the exception of phosphatidylcholine, the synthesis of phospholipids was generally stimulated by lidocaine perfusion. In contrast, hypoxia caused a general decrease in phospholipid biosynthesis which was partially restored by lidocaine. ATP and CTP levels were severely reduced under hypoxic conditions, but their levels were not restored by lidocaine treatment. The activities of enzymes for phospholipid synthesis were determined under the various perfusion conditions. The activity of phosphatidic acid phosphatase was elevated by lidocaine and decreased by hypoxic treatment. The activity of CTP:phosphatidic acid cytidylyltransferase was increased under hypoxia, with or without lidocaine. Despite the reduction in phosphatidylcholine biosynthesis, no change in the activity of cytidine diphosphocholine (CDPcholine):diacylglycerol cholinephosphotransferase was detected following lidocaine or hypoxic perfusion. However, enzyme activity was inhibited by the presence of lidocaine in the assay mixture. Our results indicate that the reduction in phospholipid biosynthesis under hypoxicconditions was caused mainly by diminishing high-energy nucleotide levels. The enhancement of phospholipid biosynthesis by lidocaine appeared to be mediated in part by modulation of enzyme activities.en_US
dc.languageengen_US
dc.publisherA O C S Press. The Journal's web site is located at http://www.aocs.org/press/lipids.htmen_US
dc.relation.ispartofLipidsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnoxia - Metabolismen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshHeart - Drug Effectsen_US
dc.subject.meshLidocaine - Pharmacologyen_US
dc.subject.meshMesocricetusen_US
dc.subject.meshMyocardium - Metabolismen_US
dc.subject.meshPhospholipids - Biosynthesisen_US
dc.titleThe effects of lidocaine and hypoxia on phospholipid biosynthesis in the isolated hamster hearten_US
dc.typeArticleen_US
dc.identifier.emailMan, RYK:rykman@hkucc.hku.hken_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF02522463-
dc.identifier.pmid8898305-
dc.identifier.scopuseid_2-s2.0-0029792970en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029792970&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume31en_US
dc.identifier.issue10en_US
dc.identifier.spage1059en_US
dc.identifier.epage1067en_US
dc.identifier.isiWOS:A1996VL90700007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, JT=36771977000en_US
dc.identifier.scopusauthoridMan, RYK=7004986435en_US
dc.identifier.scopusauthoridChoy, PC=7006633002en_US

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