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Article: Pertussis toxin-sensitive G proteins in regenerated endothelial cells of porcine coronary artery

TitlePertussis toxin-sensitive G proteins in regenerated endothelial cells of porcine coronary artery
Authors
Issue Date1994
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 1994, v. 267 n. 3 36-3, p. H979-H981 How to Cite?
AbstractEndothelium-dependent, pertussis toxin-sensitive relaxations are impaired selectively after regeneration of endothelial cells following balloon denudation of the porcine coronary artery. The present study was designed to examine the hypothesis that there is a difference in G proteins modified by pertussis toxin between regenerated and intact endothelial cells. Yorkshire pigs, fed a high-cholesterol diet, underwent balloon denudation of the endothelium of the left anterior descending coronary arteries (LAD). Four weeks after the denudation the animals were killed to detect G proteins by ADP ribosylation catalyzed with pertussis toxin and [32P]NAD, separated on a urea gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In membrane fractions of endothelial cells obtained from previously denuded LAD, Gα(i-1)/Gα(i-3) (41 kDa) and Gα(i-2) (40 kDa) proteins were labeled. The two bands revealed on the gel were the same as those obtained from intact left circumflex coronary arteries (LCX). However, the intensity of the bands was less prominent in the LAD than the LCX. These results suggest that either a decreased amount or a reduced functionality of G(i) proteins in the regenerated endothelial cells may account for the impairment in the pertussis toxin-sensitive relaxations after balloon injury of coronary arteries in the pigs.
Persistent Identifierhttp://hdl.handle.net/10722/171123
ISSN
2015 Impact Factor: 3.324
2015 SCImago Journal Rankings: 1.823
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShibano, Ten_US
dc.contributor.authorCodina, Jen_US
dc.contributor.authorBirnbaumer, Len_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:17Z-
dc.date.available2012-10-30T06:12:17Z-
dc.date.issued1994en_US
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 1994, v. 267 n. 3 36-3, p. H979-H981en_US
dc.identifier.issn0363-6135en_US
dc.identifier.urihttp://hdl.handle.net/10722/171123-
dc.description.abstractEndothelium-dependent, pertussis toxin-sensitive relaxations are impaired selectively after regeneration of endothelial cells following balloon denudation of the porcine coronary artery. The present study was designed to examine the hypothesis that there is a difference in G proteins modified by pertussis toxin between regenerated and intact endothelial cells. Yorkshire pigs, fed a high-cholesterol diet, underwent balloon denudation of the endothelium of the left anterior descending coronary arteries (LAD). Four weeks after the denudation the animals were killed to detect G proteins by ADP ribosylation catalyzed with pertussis toxin and [32P]NAD, separated on a urea gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In membrane fractions of endothelial cells obtained from previously denuded LAD, Gα(i-1)/Gα(i-3) (41 kDa) and Gα(i-2) (40 kDa) proteins were labeled. The two bands revealed on the gel were the same as those obtained from intact left circumflex coronary arteries (LCX). However, the intensity of the bands was less prominent in the LAD than the LCX. These results suggest that either a decreased amount or a reduced functionality of G(i) proteins in the regenerated endothelial cells may account for the impairment in the pertussis toxin-sensitive relaxations after balloon injury of coronary arteries in the pigs.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_US
dc.subject.meshAdenosine Diphosphate Ribose - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBalloon Dilationen_US
dc.subject.meshCoronary Vessels - Cytology - Metabolism - Physiologyen_US
dc.subject.meshEndothelium, Vascular - Cytology - Metabolism - Physiologyen_US
dc.subject.meshGtp-Binding Proteins - Metabolismen_US
dc.subject.meshHistological Techniquesen_US
dc.subject.meshPertussis Toxinen_US
dc.subject.meshRegenerationen_US
dc.subject.meshSwineen_US
dc.subject.meshVirulence Factors, Bordetella - Pharmacologyen_US
dc.titlePertussis toxin-sensitive G proteins in regenerated endothelial cells of porcine coronary arteryen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8092301-
dc.identifier.scopuseid_2-s2.0-0028018180en_US
dc.identifier.volume267en_US
dc.identifier.issue3 36-3en_US
dc.identifier.spageH979en_US
dc.identifier.epageH981en_US
dc.identifier.isiWOS:A1994PH38300016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridShibano, T=7006946465en_US
dc.identifier.scopusauthoridCodina, J=7005011473en_US
dc.identifier.scopusauthoridBirnbaumer, L=35401284700en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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