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- Scopus: eid_2-s2.0-0028018180
- PMID: 8092301
- WOS: WOS:A1994PH38300016
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Article: Pertussis toxin-sensitive G proteins in regenerated endothelial cells of porcine coronary artery
Title | Pertussis toxin-sensitive G proteins in regenerated endothelial cells of porcine coronary artery |
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Authors | |
Keywords | adenosine 5'-diphosphate balloon denudation cholesterol G(i) proteins |
Issue Date | 1994 |
Publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/ |
Citation | American Journal Of Physiology - Heart And Circulatory Physiology, 1994, v. 267 n. 3 36-3, p. H979-H981 How to Cite? |
Abstract | Endothelium-dependent, pertussis toxin-sensitive relaxations are impaired selectively after regeneration of endothelial cells following balloon denudation of the porcine coronary artery. The present study was designed to examine the hypothesis that there is a difference in G proteins modified by pertussis toxin between regenerated and intact endothelial cells. Yorkshire pigs, fed a high-cholesterol diet, underwent balloon denudation of the endothelium of the left anterior descending coronary arteries (LAD). Four weeks after the denudation the animals were killed to detect G proteins by ADP ribosylation catalyzed with pertussis toxin and [32P]NAD, separated on a urea gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In membrane fractions of endothelial cells obtained from previously denuded LAD, Gα(i-1)/Gα(i-3) (41 kDa) and Gα(i-2) (40 kDa) proteins were labeled. The two bands revealed on the gel were the same as those obtained from intact left circumflex coronary arteries (LCX). However, the intensity of the bands was less prominent in the LAD than the LCX. These results suggest that either a decreased amount or a reduced functionality of G(i) proteins in the regenerated endothelial cells may account for the impairment in the pertussis toxin-sensitive relaxations after balloon injury of coronary arteries in the pigs. |
Persistent Identifier | http://hdl.handle.net/10722/171123 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.452 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shibano, T | en_US |
dc.contributor.author | Codina, J | en_US |
dc.contributor.author | Birnbaumer, L | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:12:17Z | - |
dc.date.available | 2012-10-30T06:12:17Z | - |
dc.date.issued | 1994 | en_US |
dc.identifier.citation | American Journal Of Physiology - Heart And Circulatory Physiology, 1994, v. 267 n. 3 36-3, p. H979-H981 | en_US |
dc.identifier.issn | 0363-6135 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171123 | - |
dc.description.abstract | Endothelium-dependent, pertussis toxin-sensitive relaxations are impaired selectively after regeneration of endothelial cells following balloon denudation of the porcine coronary artery. The present study was designed to examine the hypothesis that there is a difference in G proteins modified by pertussis toxin between regenerated and intact endothelial cells. Yorkshire pigs, fed a high-cholesterol diet, underwent balloon denudation of the endothelium of the left anterior descending coronary arteries (LAD). Four weeks after the denudation the animals were killed to detect G proteins by ADP ribosylation catalyzed with pertussis toxin and [32P]NAD, separated on a urea gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In membrane fractions of endothelial cells obtained from previously denuded LAD, Gα(i-1)/Gα(i-3) (41 kDa) and Gα(i-2) (40 kDa) proteins were labeled. The two bands revealed on the gel were the same as those obtained from intact left circumflex coronary arteries (LCX). However, the intensity of the bands was less prominent in the LAD than the LCX. These results suggest that either a decreased amount or a reduced functionality of G(i) proteins in the regenerated endothelial cells may account for the impairment in the pertussis toxin-sensitive relaxations after balloon injury of coronary arteries in the pigs. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Heart and Circulatory Physiology | en_US |
dc.subject | adenosine 5'-diphosphate | - |
dc.subject | balloon denudation | - |
dc.subject | cholesterol | - |
dc.subject | G(i) proteins | - |
dc.subject.mesh | Adenosine Diphosphate Ribose - Metabolism | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Balloon Dilation | en_US |
dc.subject.mesh | Coronary Vessels - Cytology - Metabolism - Physiology | en_US |
dc.subject.mesh | Endothelium, Vascular - Cytology - Metabolism - Physiology | en_US |
dc.subject.mesh | Gtp-Binding Proteins - Metabolism | en_US |
dc.subject.mesh | Histological Techniques | en_US |
dc.subject.mesh | Pertussis Toxin | en_US |
dc.subject.mesh | Regeneration | en_US |
dc.subject.mesh | Swine | en_US |
dc.subject.mesh | Virulence Factors, Bordetella - Pharmacology | en_US |
dc.title | Pertussis toxin-sensitive G proteins in regenerated endothelial cells of porcine coronary artery | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 8092301 | - |
dc.identifier.scopus | eid_2-s2.0-0028018180 | en_US |
dc.identifier.volume | 267 | en_US |
dc.identifier.issue | 3 36-3 | en_US |
dc.identifier.spage | H979 | en_US |
dc.identifier.epage | H981 | en_US |
dc.identifier.isi | WOS:A1994PH38300016 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Shibano, T=7006946465 | en_US |
dc.identifier.scopusauthorid | Codina, J=7005011473 | en_US |
dc.identifier.scopusauthorid | Birnbaumer, L=35401284700 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0363-6135 | - |