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Article: Induction of NO production by TNF-α and lipopolysaccharide in porcine coronary arteries without endothelium

TitleInduction of NO production by TNF-α and lipopolysaccharide in porcine coronary arteries without endothelium
Authors
Issue Date1993
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 2 33-2, p. H403-H407 How to Cite?
AbstractThe effects of tumor necrosis factor-α (TNF-α) and lipopolysaccharide (LPS) were studied in porcine coronary arteries without endothelium. Rings of the artery were incubated in minimum essential medium with TNF-α or LPS for 6 or 24 h. After 6 h of incubation, the rings were suspended in organ chambers filled with physiological salt solution containing indomethacin for the measurement of isometric force. The rings were contracted with prostaglandin F(2α) before the addition of L-arginine. In rings treated with TNF-α or LPS, L-arginine caused a concentration-dependent relaxation that was abolished by N(ω)-nitro-L-arginine [an inhibitor of nitric oxide (NO) synthase]. However, contractions to 5-hydroxytryptamine were not affected by TNF-α and LPS. After 24 h of incubation, TNF and LPS impaired the contractions to 5-hydroxytryptamine and increased the accumulation of nitrite, a stable degradation product of NO. These effects of TNF-α and LPS were blocked by N(ω)-nitro-L-arginine. Cycloheximide (an inhibitor of protein synthesis) attenuated the inhibitory effect of TNF-α and LPS on contractions to 5-hydroxytryptamine. Thus, in the porcine coronary artery without endothelium, TNF-α and LPS can induce an L-arginine-NO pathway.
Persistent Identifierhttp://hdl.handle.net/10722/171104
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShibano, Ten_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:12Z-
dc.date.available2012-10-30T06:12:12Z-
dc.date.issued1993en_US
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 1993, v. 264 n. 2 33-2, p. H403-H407en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/171104-
dc.description.abstractThe effects of tumor necrosis factor-α (TNF-α) and lipopolysaccharide (LPS) were studied in porcine coronary arteries without endothelium. Rings of the artery were incubated in minimum essential medium with TNF-α or LPS for 6 or 24 h. After 6 h of incubation, the rings were suspended in organ chambers filled with physiological salt solution containing indomethacin for the measurement of isometric force. The rings were contracted with prostaglandin F(2α) before the addition of L-arginine. In rings treated with TNF-α or LPS, L-arginine caused a concentration-dependent relaxation that was abolished by N(ω)-nitro-L-arginine [an inhibitor of nitric oxide (NO) synthase]. However, contractions to 5-hydroxytryptamine were not affected by TNF-α and LPS. After 24 h of incubation, TNF and LPS impaired the contractions to 5-hydroxytryptamine and increased the accumulation of nitrite, a stable degradation product of NO. These effects of TNF-α and LPS were blocked by N(ω)-nitro-L-arginine. Cycloheximide (an inhibitor of protein synthesis) attenuated the inhibitory effect of TNF-α and LPS on contractions to 5-hydroxytryptamine. Thus, in the porcine coronary artery without endothelium, TNF-α and LPS can induce an L-arginine-NO pathway.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArginine - Pharmacologyen_US
dc.subject.meshCoronary Vessels - Drug Effects - Metabolismen_US
dc.subject.meshEndothelium, Vascular - Physiologyen_US
dc.subject.meshLipopolysaccharides - Pharmacologyen_US
dc.subject.meshNitric Oxide - Metabolismen_US
dc.subject.meshNitrites - Metabolismen_US
dc.subject.meshSerotonin - Pharmacologyen_US
dc.subject.meshSwineen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Pharmacologyen_US
dc.subject.meshVasodilationen_US
dc.titleInduction of NO production by TNF-α and lipopolysaccharide in porcine coronary arteries without endotheliumen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8447456-
dc.identifier.scopuseid_2-s2.0-0027419488en_US
dc.identifier.volume264en_US
dc.identifier.issue2 33-2en_US
dc.identifier.spageH403en_US
dc.identifier.epageH407en_US
dc.identifier.isiWOS:A1993KN68100017-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridShibano, T=7006946465en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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