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Article: Respiratory epithelium modulates the responses of canine bronchi to cooling

TitleRespiratory epithelium modulates the responses of canine bronchi to cooling
Authors
Issue Date1993
Citation
Journal Of Applied Physiology, 1993, v. 74 n. 5, p. 2421-2425 How to Cite?
AbstractThe present study was designed to investigate the effect of cooling on the modulatory role of the respiratory epithelium on the underlying smooth muscle. Canine bronchial rings and segments (with or without epithelium) were suspended in organ chambers and perfused with modified Krebs-Ringer bicarbonate solution, respectively. Isometric force was recorded. Cooling did not affect the resting tension of the bronchi. During contractions to carbachol, cooling evoked contractions in bronchi with epithelium but relaxations in those without epithelium. In the presence of indomethacin, cooling induced contractions in both preparations with and without epithelium. The contractions in bronchi with epithelium were significantly larger than those in bronchi without epithelium. After treatment with indomethacin, exogenous arachidonic acid potentiated the cooling-induced contractions in preparations with epithelium but not in those without epithelium. This potentiation was not affected by nordihydroguaiaretic acid. SKF 525-A and metyrapone, inhibitors of cytochrome P-450 monooxygenases, converted the cooling-induced contractions of preparations with epithelium to relaxations and had no significant effects on the responses of preparations without epithelium. These observations suggest that cooling induces from the epithelium the release of a cytochrome P-450-derived eicosanoid that potentiates contractions of the underlying airway smooth muscle to carbachol.
Persistent Identifierhttp://hdl.handle.net/10722/171086
ISSN
2015 Impact Factor: 3.004
2015 SCImago Journal Rankings: 1.488
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGao, Yen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:12:08Z-
dc.date.available2012-10-30T06:12:08Z-
dc.date.issued1993en_US
dc.identifier.citationJournal Of Applied Physiology, 1993, v. 74 n. 5, p. 2421-2425en_US
dc.identifier.issn8750-7587en_US
dc.identifier.urihttp://hdl.handle.net/10722/171086-
dc.description.abstractThe present study was designed to investigate the effect of cooling on the modulatory role of the respiratory epithelium on the underlying smooth muscle. Canine bronchial rings and segments (with or without epithelium) were suspended in organ chambers and perfused with modified Krebs-Ringer bicarbonate solution, respectively. Isometric force was recorded. Cooling did not affect the resting tension of the bronchi. During contractions to carbachol, cooling evoked contractions in bronchi with epithelium but relaxations in those without epithelium. In the presence of indomethacin, cooling induced contractions in both preparations with and without epithelium. The contractions in bronchi with epithelium were significantly larger than those in bronchi without epithelium. After treatment with indomethacin, exogenous arachidonic acid potentiated the cooling-induced contractions in preparations with epithelium but not in those without epithelium. This potentiation was not affected by nordihydroguaiaretic acid. SKF 525-A and metyrapone, inhibitors of cytochrome P-450 monooxygenases, converted the cooling-induced contractions of preparations with epithelium to relaxations and had no significant effects on the responses of preparations without epithelium. These observations suggest that cooling induces from the epithelium the release of a cytochrome P-450-derived eicosanoid that potentiates contractions of the underlying airway smooth muscle to carbachol.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArachidonic Acid - Pharmacologyen_US
dc.subject.meshBronchi - Drug Effects - Enzymology - Physiologyen_US
dc.subject.meshBronchoconstriction - Drug Effects - Physiologyen_US
dc.subject.meshCarbachol - Pharmacologyen_US
dc.subject.meshCold Temperature - Adverse Effectsen_US
dc.subject.meshCytochrome P-450 Enzyme System - Antagonists & Inhibitors - Metabolismen_US
dc.subject.meshDogsen_US
dc.subject.meshEicosanoids - Metabolismen_US
dc.subject.meshEpithelium - Enzymology - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshIsometric Contraction - Drug Effects - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshOuabain - Pharmacologyen_US
dc.subject.meshPerfusionen_US
dc.titleRespiratory epithelium modulates the responses of canine bronchi to coolingen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8335576-
dc.identifier.scopuseid_2-s2.0-0027231012en_US
dc.identifier.volume74en_US
dc.identifier.issue5en_US
dc.identifier.spage2421en_US
dc.identifier.epage2425en_US
dc.identifier.isiWOS:A1993LD69900052-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGao, Y=7404706442en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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