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Article: Endothelium-dependent relaxations of piglet pulmonary arteries augment with maturation

TitleEndothelium-dependent relaxations of piglet pulmonary arteries augment with maturation
Authors
Issue Date1991
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
Citation
Pediatric Research, 1991, v. 30 n. 2, p. 176-180 How to Cite?
AbstractTo determine whether maturation alters endothelium-dependent responses in porcine pulmonary arteries, rings, with and without endothelium, of small pulmonary arteries taken from piglets of 3, 10, and 30 d of age were suspended in organ chambers filled with buffered salt solution, bubbled with 95% O2-5% CO2, and maintained at 37°C. These studies were performed in the presence of indomethacin (10-5 M) to inhibit prostaglandin synthesis. In rings without endothelium, potassium chloride (10-2 to 8.5 x 10-2 M) and histamine (10-9 to 10-5 M) caused concentration-dependent contractions. When normalized to maximal contractions achieved to each agonist, the concentration-effect curves to potassium chloride and histamine in rings without endothelium were similar at each age. Rings with endothelium showed a progressive shift to the right of the concentration-effect curve to histamine, possibly secondary to an increase in the basal release of, or responsiveness to, the endothelium-derived relaxing factor with maturation. Relaxations to sodium nitroprusside (10-9 to 10-5 M) were unaffected by age. In precontracted rings, acetylcholine (10-9 to 10-6 M), bradykinin (10-10 to 10-6 M), and the calcium ionophore A23187 (10-9 to 10-6 M) caused relaxations in rings with endothelium, but not in those without endothelium, which were greater at 10 and 30 d compared to 3 d; further augmentation at 30 d compared to 10 d was not observed. In rings without endothelium, changes in the responsiveness to nitric oxide (10-9 to 10-5 M), one of the proposed endothelium-derived relaxing factors, with age were comparable to those observed with endothelium-dependent relaxing agents. These studies demonstrate that endothelium-dependent relaxations increase with age, possibly due to changes in sensitivity of the smooth muscle to the endothelium-derived relaxing factor.
Persistent Identifierhttp://hdl.handle.net/10722/171042
ISSN
2015 Impact Factor: 2.761
2015 SCImago Journal Rankings: 1.290
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZellers, TMen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:57Z-
dc.date.available2012-10-30T06:11:57Z-
dc.date.issued1991en_US
dc.identifier.citationPediatric Research, 1991, v. 30 n. 2, p. 176-180en_US
dc.identifier.issn0031-3998en_US
dc.identifier.urihttp://hdl.handle.net/10722/171042-
dc.description.abstractTo determine whether maturation alters endothelium-dependent responses in porcine pulmonary arteries, rings, with and without endothelium, of small pulmonary arteries taken from piglets of 3, 10, and 30 d of age were suspended in organ chambers filled with buffered salt solution, bubbled with 95% O2-5% CO2, and maintained at 37°C. These studies were performed in the presence of indomethacin (10-5 M) to inhibit prostaglandin synthesis. In rings without endothelium, potassium chloride (10-2 to 8.5 x 10-2 M) and histamine (10-9 to 10-5 M) caused concentration-dependent contractions. When normalized to maximal contractions achieved to each agonist, the concentration-effect curves to potassium chloride and histamine in rings without endothelium were similar at each age. Rings with endothelium showed a progressive shift to the right of the concentration-effect curve to histamine, possibly secondary to an increase in the basal release of, or responsiveness to, the endothelium-derived relaxing factor with maturation. Relaxations to sodium nitroprusside (10-9 to 10-5 M) were unaffected by age. In precontracted rings, acetylcholine (10-9 to 10-6 M), bradykinin (10-10 to 10-6 M), and the calcium ionophore A23187 (10-9 to 10-6 M) caused relaxations in rings with endothelium, but not in those without endothelium, which were greater at 10 and 30 d compared to 3 d; further augmentation at 30 d compared to 10 d was not observed. In rings without endothelium, changes in the responsiveness to nitric oxide (10-9 to 10-5 M), one of the proposed endothelium-derived relaxing factors, with age were comparable to those observed with endothelium-dependent relaxing agents. These studies demonstrate that endothelium-dependent relaxations increase with age, possibly due to changes in sensitivity of the smooth muscle to the endothelium-derived relaxing factor.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/en_US
dc.relation.ispartofPediatric Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshFemaleen_US
dc.subject.meshHistamine - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effectsen_US
dc.subject.meshNitric Oxide - Physiologyen_US
dc.subject.meshNitroprusside - Pharmacologyen_US
dc.subject.meshPotassium Chloride - Pharmacologyen_US
dc.subject.meshPulmonary Artery - Growth & Development - Ultrastructureen_US
dc.subject.meshSwineen_US
dc.subject.meshVasoconstriction - Drug Effectsen_US
dc.titleEndothelium-dependent relaxations of piglet pulmonary arteries augment with maturationen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1203/00006450-199108000-00011-
dc.identifier.pmid1896263-
dc.identifier.scopuseid_2-s2.0-0026350137en_US
dc.identifier.volume30en_US
dc.identifier.issue2en_US
dc.identifier.spage176en_US
dc.identifier.epage180en_US
dc.identifier.isiWOS:A1991FY61100010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZellers, TM=6701423788en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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