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Article: Ouabain, Na+-free and K+-free solutions and relaxations to nitric oxide and nitrovasodilators

TitleOuabain, Na+-free and K+-free solutions and relaxations to nitric oxide and nitrovasodilators
Authors
Issue Date1991
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/vph
Citation
General Pharmacology, 1991, v. 22 n. 2, p. 337-340 How to Cite?
Abstract1. Experiments were designed to determine the effect of inhibition of Na+, K+ ATPase by ouabain, Na+-free and K+-free solutions on the relaxations induced by nitric oxide, nitroglycerin and sodium nitropruside in coronary smooth muscle. 2. Rings without endothelium of canine life anterior descending coronary artery were suspended for isometric tension recording in modified Krebs-Ringer bicarbonate solution, in the presence of indomethacin, propranolol, and phenoxybenzamine. The rings were incubated with ouabain, Na+-free or K+-free solutions prior to contractions with prostaglandin F(2α). 3. Nitric oxide induced transient relaxations which were significantly depressed by ouabain but were not affected by Na+- or K+-free solutions. Thus, the inhibitory effect of ouabain on relaxations to nitric oxide is not due to inhibition of the Na+, K+ pump. 4. The relaxation indued by sodium nitroprusside was impaired both in the presence of ouabain and after incubation in Na+- or K+-free solution, suggesting that it may involve in part activation of Na+, K+ pumping. 5. Only high concentration of ouabain and Na+-free solution decreaed the relaxation evoked by nitroglycerin, indicating that the mechanism of action of nitroglycerin on vascular smooth muscle does not involve the Na+-K+ pump.
Persistent Identifierhttp://hdl.handle.net/10722/171021
ISSN
2002 Impact Factor: 0.591
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBoulanger, Cen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:51Z-
dc.date.available2012-10-30T06:11:51Z-
dc.date.issued1991en_US
dc.identifier.citationGeneral Pharmacology, 1991, v. 22 n. 2, p. 337-340en_US
dc.identifier.issn0306-3623en_US
dc.identifier.urihttp://hdl.handle.net/10722/171021-
dc.description.abstract1. Experiments were designed to determine the effect of inhibition of Na+, K+ ATPase by ouabain, Na+-free and K+-free solutions on the relaxations induced by nitric oxide, nitroglycerin and sodium nitropruside in coronary smooth muscle. 2. Rings without endothelium of canine life anterior descending coronary artery were suspended for isometric tension recording in modified Krebs-Ringer bicarbonate solution, in the presence of indomethacin, propranolol, and phenoxybenzamine. The rings were incubated with ouabain, Na+-free or K+-free solutions prior to contractions with prostaglandin F(2α). 3. Nitric oxide induced transient relaxations which were significantly depressed by ouabain but were not affected by Na+- or K+-free solutions. Thus, the inhibitory effect of ouabain on relaxations to nitric oxide is not due to inhibition of the Na+, K+ pump. 4. The relaxation indued by sodium nitroprusside was impaired both in the presence of ouabain and after incubation in Na+- or K+-free solution, suggesting that it may involve in part activation of Na+, K+ pumping. 5. Only high concentration of ouabain and Na+-free solution decreaed the relaxation evoked by nitroglycerin, indicating that the mechanism of action of nitroglycerin on vascular smooth muscle does not involve the Na+-K+ pump.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/vphen_US
dc.relation.ispartofGeneral Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCoronary Vessels - Drug Effectsen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshIsometric Contraction - Drug Effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshMuscle Relaxation - Drug Effectsen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effectsen_US
dc.subject.meshNitric Oxide - Pharmacologyen_US
dc.subject.meshNitroglycerin - Pharmacologyen_US
dc.subject.meshNitroprusside - Pharmacologyen_US
dc.subject.meshOuabain - Pharmacologyen_US
dc.subject.meshPotassium - Physiologyen_US
dc.subject.meshSodium - Physiologyen_US
dc.subject.meshSodium-Potassium-Exchanging Atpase - Antagonists & Inhibitorsen_US
dc.subject.meshVasodilator Agents - Pharmacologyen_US
dc.titleOuabain, Na+-free and K+-free solutions and relaxations to nitric oxide and nitrovasodilatorsen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0306-3623(91)90460-N-
dc.identifier.pmid1647345-
dc.identifier.scopuseid_2-s2.0-0025977310en_US
dc.identifier.volume22en_US
dc.identifier.issue2en_US
dc.identifier.spage337en_US
dc.identifier.epage340en_US
dc.identifier.isiWOS:A1991EY11500027-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBoulanger, C=7006599024en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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