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Article: The impairment of endothelium-dependent relaxations in reversed vein grafts is associated with a reduced production of cyclic guanosine monophosphate

TitleThe impairment of endothelium-dependent relaxations in reversed vein grafts is associated with a reduced production of cyclic guanosine monophosphate
Authors
Issue Date1991
PublisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/jvs
Citation
Journal Of Vascular Surgery, 1991, v. 14 n. 1, p. 67-75 How to Cite?
AbstractThe present study investigated the underlying mechanisms associated with the loss of responsiveness of veins grafted into the arterial circulation. In particular, the possibility that the altered response is related to modifications of the biologic properties of the vascular smooth muscle, of the endothelial cells or both was tested. Segments of jugular veins were grafted in the reverse position into the carotid arteries in rabbits. After 4 weeks the patent vein grafts and unoperated veins were removed, and endothelium-dependent (acetylcholine) and endothelium-independent (nitric oxide, SIN-1 [the active metabolite of molsidomine32]) relaxations were studied in vitro. In unoperated veins, acetylcholine, nitric oxide, and SIN-1 induced a concentration-dependent relaxation in the presence and absence of the endothelium, respectively. These relaxations were associated with a time-dependent accumulation of guanosine 3':5'-cyclic monophosphate (cyclic GMP). Both relaxation and production of cyclic GMP were inhibited by methylene blue and hemoglobin. Unstimulated veins with endothelium had a significantly higher content of cyclic GMP than did preparations without endothelial cells. This difference was abolished by hemoglobin and methylene blue. In vein grafts acetylcholine induced only minor endothelium-dependent relaxations, whereas nitric oxide and SIN-1 evoked concentration-dependent relaxations in preparations without endothelium, which were shifted significantly to the right compared to unoperated veins. In vein grafts the endothelium-mediated production of cyclic GMP (basal and stimulated by acetylcholine) was significantly reduced when compared to that in unoperated veins, and that evoked by SIN-1 was not different. These results demonstrate that the production of endothelium-derived nitric oxide by endothelial cells is markedly impaired in vein grafts and also that the responsiveness of the smooth muscle is reduced, although to a smaller extent. These altered biologic responses of the grafted veins may contribute to graft failure.
Persistent Identifierhttp://hdl.handle.net/10722/171013
ISSN
2015 Impact Factor: 3.454
2015 SCImago Journal Rankings: 2.115

 

DC FieldValueLanguage
dc.contributor.authorKomori, Ken_US
dc.contributor.authorSchini, VBen_US
dc.contributor.authorGloviczki, Pen_US
dc.contributor.authorBourchier, RGen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:50Z-
dc.date.available2012-10-30T06:11:50Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of Vascular Surgery, 1991, v. 14 n. 1, p. 67-75en_US
dc.identifier.issn0741-5214en_US
dc.identifier.urihttp://hdl.handle.net/10722/171013-
dc.description.abstractThe present study investigated the underlying mechanisms associated with the loss of responsiveness of veins grafted into the arterial circulation. In particular, the possibility that the altered response is related to modifications of the biologic properties of the vascular smooth muscle, of the endothelial cells or both was tested. Segments of jugular veins were grafted in the reverse position into the carotid arteries in rabbits. After 4 weeks the patent vein grafts and unoperated veins were removed, and endothelium-dependent (acetylcholine) and endothelium-independent (nitric oxide, SIN-1 [the active metabolite of molsidomine32]) relaxations were studied in vitro. In unoperated veins, acetylcholine, nitric oxide, and SIN-1 induced a concentration-dependent relaxation in the presence and absence of the endothelium, respectively. These relaxations were associated with a time-dependent accumulation of guanosine 3':5'-cyclic monophosphate (cyclic GMP). Both relaxation and production of cyclic GMP were inhibited by methylene blue and hemoglobin. Unstimulated veins with endothelium had a significantly higher content of cyclic GMP than did preparations without endothelial cells. This difference was abolished by hemoglobin and methylene blue. In vein grafts acetylcholine induced only minor endothelium-dependent relaxations, whereas nitric oxide and SIN-1 evoked concentration-dependent relaxations in preparations without endothelium, which were shifted significantly to the right compared to unoperated veins. In vein grafts the endothelium-mediated production of cyclic GMP (basal and stimulated by acetylcholine) was significantly reduced when compared to that in unoperated veins, and that evoked by SIN-1 was not different. These results demonstrate that the production of endothelium-derived nitric oxide by endothelial cells is markedly impaired in vein grafts and also that the responsiveness of the smooth muscle is reduced, although to a smaller extent. These altered biologic responses of the grafted veins may contribute to graft failure.en_US
dc.languageengen_US
dc.publisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/locate/jvsen_US
dc.relation.ispartofJournal of Vascular Surgeryen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarotid Arteries - Surgeryen_US
dc.subject.meshCyclic Gmp - Biosynthesisen_US
dc.subject.meshEndothelium, Vascular - Physiologyen_US
dc.subject.meshHemoglobins - Pharmacologyen_US
dc.subject.meshJugular Veins - Transplantationen_US
dc.subject.meshMaleen_US
dc.subject.meshMolsidomine - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshMuscle Relaxation - Drug Effects - Physiologyen_US
dc.subject.meshNitric Oxide - Physiologyen_US
dc.subject.meshRabbitsen_US
dc.subject.meshVasodilator Agents - Pharmacologyen_US
dc.subject.meshVeins - Metabolism - Physiology - Transplantationen_US
dc.titleThe impairment of endothelium-dependent relaxations in reversed vein grafts is associated with a reduced production of cyclic guanosine monophosphateen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1067/mva.1991.28411en_US
dc.identifier.pmid1648144-
dc.identifier.scopuseid_2-s2.0-0025912719en_US
dc.identifier.volume14en_US
dc.identifier.issue1en_US
dc.identifier.spage67en_US
dc.identifier.epage75en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKomori, K=8977740100en_US
dc.identifier.scopusauthoridSchini, VB=7004113565en_US
dc.identifier.scopusauthoridGloviczki, P=7102379868en_US
dc.identifier.scopusauthoridBourchier, RG=6603676592en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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