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Article: Chronic exposure of cultured endothelial cells to eicosapentaenoic acid potentiates the release of endothelium-derived relaxing factor(s)

TitleChronic exposure of cultured endothelial cells to eicosapentaenoic acid potentiates the release of endothelium-derived relaxing factor(s)
Authors
Issue Date1990
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 1990, v. 99 n. 1, p. 176-180 How to Cite?
AbstractThe effect of chronic exposure of cultured porcine aortic endothelial cells to eicosapentaenoic acid on the release of indomethacin-insensitive relaxing factor(s) was investigated (a) under bioassay conditions using preconstricted canine coronary artery rings without endothelium and (b) by the measurement of guanosine 3':5'-cyclic monophosphate (cyclic GMP) content of endothelial cells. Exposure of endothelial cells for 8-10 days to eicosapentaenoic acid (2.5 x 10-5 M) did not affect the relaxing activity of the perfusate from unstimulated endothelial cells. The treatment with eicosapentaenoic acid significantly increased the relaxation of the bioassay ring observed upon stimulation of the endothelial cells with adenosine diphosphate (3 x 10-8 M to 3 x 10-4 M) and, to a lesser extent with bradykinin (10-8 to 3 x 10-8 M), while the relaxing activity evoked by the calcium ionophore A23187 (3 x 10-7 M) was not affected. Neither acetylcholine (10-6 M) nor 5-hydroxytryptamine (10-6 M) stimulated the release of relaxing factor(s) from control or eicosapentaenoic acid-treated endothelial cells. Bradykinin (10-7 M), adenosine diphosphate (3 x 10-5 M), the calcium ionophore A23187 (10-6 M) and nitric oxide (2 x 10-6 M) stimulated haemoglobin-sensitive increases in cyclic GMP content of porcine endothelial cells which were unaffected by prior chronic exposure to eicosapentaenoic acid. These results suggest that chronic exposure of porcine aortic endothelial cells to eicosapentaenoic acid increases the release of relaxing factor(s) in response to activation of membrane-associated receptors for purines and kinins. The lack of effect of eicosapentaenoic acid treatment on agonist-stimulated production of cyclic GMP suggests that the enhanced relaxation observed under bioassay conditions is not due to an increased production of nitric oxide.
Persistent Identifierhttp://hdl.handle.net/10722/170975
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBoulanger, Cen_US
dc.contributor.authorSchini, VBen_US
dc.contributor.authorHendrickson, Hen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:40Z-
dc.date.available2012-10-30T06:11:40Z-
dc.date.issued1990en_US
dc.identifier.citationBritish Journal Of Pharmacology, 1990, v. 99 n. 1, p. 176-180en_US
dc.identifier.issn0007-1188en_US
dc.identifier.urihttp://hdl.handle.net/10722/170975-
dc.description.abstractThe effect of chronic exposure of cultured porcine aortic endothelial cells to eicosapentaenoic acid on the release of indomethacin-insensitive relaxing factor(s) was investigated (a) under bioassay conditions using preconstricted canine coronary artery rings without endothelium and (b) by the measurement of guanosine 3':5'-cyclic monophosphate (cyclic GMP) content of endothelial cells. Exposure of endothelial cells for 8-10 days to eicosapentaenoic acid (2.5 x 10-5 M) did not affect the relaxing activity of the perfusate from unstimulated endothelial cells. The treatment with eicosapentaenoic acid significantly increased the relaxation of the bioassay ring observed upon stimulation of the endothelial cells with adenosine diphosphate (3 x 10-8 M to 3 x 10-4 M) and, to a lesser extent with bradykinin (10-8 to 3 x 10-8 M), while the relaxing activity evoked by the calcium ionophore A23187 (3 x 10-7 M) was not affected. Neither acetylcholine (10-6 M) nor 5-hydroxytryptamine (10-6 M) stimulated the release of relaxing factor(s) from control or eicosapentaenoic acid-treated endothelial cells. Bradykinin (10-7 M), adenosine diphosphate (3 x 10-5 M), the calcium ionophore A23187 (10-6 M) and nitric oxide (2 x 10-6 M) stimulated haemoglobin-sensitive increases in cyclic GMP content of porcine endothelial cells which were unaffected by prior chronic exposure to eicosapentaenoic acid. These results suggest that chronic exposure of porcine aortic endothelial cells to eicosapentaenoic acid increases the release of relaxing factor(s) in response to activation of membrane-associated receptors for purines and kinins. The lack of effect of eicosapentaenoic acid treatment on agonist-stimulated production of cyclic GMP suggests that the enhanced relaxation observed under bioassay conditions is not due to an increased production of nitric oxide.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_US
dc.relation.ispartofBritish Journal of Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBradykinin - Pharmacologyen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Gmp - Metabolismen_US
dc.subject.meshDogsen_US
dc.subject.meshEicosapentaenoic Acid - Pharmacologyen_US
dc.subject.meshEndothelium, Vascular - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshNitric Oxide - Metabolism - Pharmacologyen_US
dc.subject.meshOxyhemoglobins - Pharmacologyen_US
dc.titleChronic exposure of cultured endothelial cells to eicosapentaenoic acid potentiates the release of endothelium-derived relaxing factor(s)en_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1476-5381.1990.tb14673.x-
dc.identifier.pmid2158833-
dc.identifier.scopuseid_2-s2.0-0025060848en_US
dc.identifier.volume99en_US
dc.identifier.issue1en_US
dc.identifier.spage176en_US
dc.identifier.epage180en_US
dc.identifier.isiWOS:A1990CJ71400034-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridBoulanger, C=7006599024en_US
dc.identifier.scopusauthoridSchini, VB=7004113565en_US
dc.identifier.scopusauthoridHendrickson, H=14121025300en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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