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Article: Endothelium-dependent relaxation in response to aggregating platelets in porcine femoral veins and its modulation by diet

TitleEndothelium-dependent relaxation in response to aggregating platelets in porcine femoral veins and its modulation by diet
Authors
Issue Date1989
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org
Citation
Circulation, 1989, v. 80 n. 2, p. 401-409 How to Cite?
AbstractThe present study examined the protective role of the venous endothelium against aggregating platelets and its modulation by diet. Yorkshire pigs were fed a regular chow (control pigs), 2% high-cholesterol diet (for 10 weeks, cholesterol-fed pigs), and regular chow plus cod-liver oil (30 ml/day for 4 weeks, oil-fed pigs). Endothelium-dependent responses were examined in vitro in rings of femoral veins in the presence of the inhibitor of cyclooxygenase indomethacin. In control pigs, aggregating platelets, serotonin, and ADP caused endothelium-dependent relaxations. The platelet-induced relaxations were attenuated by methiothepin (a combined 5-HT1 and 5-HT2 serotonergic blocker) or apyrase (an ADPase and ATPase) and were abolished by the combination of the two agents. In quiescent rings, platelet caused contractions, which were reduced in the presence of endothelium; the contractions were prevented by ketanserin (a 5-HT2 serotonergic blocker) or methiothepin but not by R 68070 (a thromboxane A2 receptor blocker) or dazoxiben (a thromboxane-synthetase blocker). In cholesterol-fed pigs, the platelet-induced relaxations were not altered, whereas in oil-fed pigs, the endothelium-dependent relaxations to platelets, serotonin, and ADP were augmented. Platelet-induced contractions were significantly reduced in rings with endothelium from oil-fed pigs, whereas the contractions were comparable in rings without endothelium among the three groups. Endothelium-dependent relaxations in response to the calcium ionophore A23187, direct relaxations in response to sodium nitroprusside, and direct contractions in response to potassium chloride were comparable among the three groups. These results indicate that 1) the endothelium exerts inhibitory effects against aggregating platelets in porcine femoral veins, 2) the relaxations are mediated by serotonin and ADP released from the aggregating platelets, 3) platelet-induced contractions are mediated by serotonin with little contribution of thromboxanes, and 4) hypercholesterolemia does not affect, but cod-liver oil facilitates, the endothelium-dependent relaxations to aggregating platelets because of augmented responses to released serotonin and ADP.
Persistent Identifierhttp://hdl.handle.net/10722/170923
ISSN
2015 Impact Factor: 17.047
2015 SCImago Journal Rankings: 7.853
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKomori, Ken_US
dc.contributor.authorShimokawa, Hen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:27Z-
dc.date.available2012-10-30T06:11:27Z-
dc.date.issued1989en_US
dc.identifier.citationCirculation, 1989, v. 80 n. 2, p. 401-409en_US
dc.identifier.issn0009-7322en_US
dc.identifier.urihttp://hdl.handle.net/10722/170923-
dc.description.abstractThe present study examined the protective role of the venous endothelium against aggregating platelets and its modulation by diet. Yorkshire pigs were fed a regular chow (control pigs), 2% high-cholesterol diet (for 10 weeks, cholesterol-fed pigs), and regular chow plus cod-liver oil (30 ml/day for 4 weeks, oil-fed pigs). Endothelium-dependent responses were examined in vitro in rings of femoral veins in the presence of the inhibitor of cyclooxygenase indomethacin. In control pigs, aggregating platelets, serotonin, and ADP caused endothelium-dependent relaxations. The platelet-induced relaxations were attenuated by methiothepin (a combined 5-HT1 and 5-HT2 serotonergic blocker) or apyrase (an ADPase and ATPase) and were abolished by the combination of the two agents. In quiescent rings, platelet caused contractions, which were reduced in the presence of endothelium; the contractions were prevented by ketanserin (a 5-HT2 serotonergic blocker) or methiothepin but not by R 68070 (a thromboxane A2 receptor blocker) or dazoxiben (a thromboxane-synthetase blocker). In cholesterol-fed pigs, the platelet-induced relaxations were not altered, whereas in oil-fed pigs, the endothelium-dependent relaxations to platelets, serotonin, and ADP were augmented. Platelet-induced contractions were significantly reduced in rings with endothelium from oil-fed pigs, whereas the contractions were comparable in rings without endothelium among the three groups. Endothelium-dependent relaxations in response to the calcium ionophore A23187, direct relaxations in response to sodium nitroprusside, and direct contractions in response to potassium chloride were comparable among the three groups. These results indicate that 1) the endothelium exerts inhibitory effects against aggregating platelets in porcine femoral veins, 2) the relaxations are mediated by serotonin and ADP released from the aggregating platelets, 3) platelet-induced contractions are mediated by serotonin with little contribution of thromboxanes, and 4) hypercholesterolemia does not affect, but cod-liver oil facilitates, the endothelium-dependent relaxations to aggregating platelets because of augmented responses to released serotonin and ADP.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.orgen_US
dc.relation.ispartofCirculationen_US
dc.subject.meshAdenosine Diphosphate - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Factors - Physiologyen_US
dc.subject.meshCholesterol, Dietary - Pharmacologyen_US
dc.subject.meshCod Liver Oil - Pharmacologyen_US
dc.subject.meshEndothelium, Vascular - Physiologyen_US
dc.subject.meshFemoral Vein - Physiologyen_US
dc.subject.meshFish Oils - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contractionen_US
dc.subject.meshMuscle, Smooth, Vascular - Physiologyen_US
dc.subject.meshNitric Oxideen_US
dc.subject.meshPlatelet Aggregationen_US
dc.subject.meshSerotonin - Physiologyen_US
dc.subject.meshSwineen_US
dc.subject.meshTime Factorsen_US
dc.titleEndothelium-dependent relaxation in response to aggregating platelets in porcine femoral veins and its modulation by dieten_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1161/01.CIR.80.2.401-
dc.identifier.pmid2787711-
dc.identifier.scopuseid_2-s2.0-0024373107en_US
dc.identifier.volume80en_US
dc.identifier.issue2en_US
dc.identifier.spage401en_US
dc.identifier.epage409en_US
dc.identifier.isiWOS:A1989AK34900021-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKomori, K=8977740100en_US
dc.identifier.scopusauthoridShimokawa, H=16684837100en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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