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Article: Removal of the epithelium potentiates acetylcholine in depolarizing canine bronchial smooth muscle

TitleRemoval of the epithelium potentiates acetylcholine in depolarizing canine bronchial smooth muscle
Authors
Issue Date1988
Citation
Journal Of Applied Physiology, 1988, v. 65 n. 6, p. 2400-2405 How to Cite?
AbstractExperiments were designed to determine whether the airway epithelium affects the membrane potential of the underlying smooth muscle. The effect of epithelium removal (by gentle rubbing) on the responsiveness of isolated canine bronchi was studied. Simultaneous recordings of mechanical and electrical activity were made in paired circumferential strips (with and without epithelium) of third-order bronchi. Changes in tension were recorded with a force transducer, and changes in membrane potential were measured with a microelectrode. The cell membrane potential and resting tension of the bronchial smooth muscle were stable over a 150-min period and were not affected by removal of the epithelium. In the presence of antagonists at muscarinic and adrenergic receptors, the resting tension and membrane potential were comparable in preparations with and without epithelium. By contrast, the anticholinesterase, echothiophate, caused depolarization in bronchi without epithelium. Exposure to high potassium induced similar levels of depolarization and contraction in tissues with and without epithelium. No significant differences in threshold for depolarization or for mechanical activation in the membrane potential-tension relationship were noted in the presence or absence of epithelium. In the presence of echothiophate, removal of the epithelium augmented the contraction of the bronchi to acetylcholine; the depolarization of the cell membrane induced by the cholinergic transmitter was significantly larger than in control tissues, even when matched contractions were compared. These observations indicate that the respiratory epithelium generates an inhibitory substance that dampens depolarization and contraction of bronchial smooth muscle caused by acetylcholine.
Persistent Identifierhttp://hdl.handle.net/10722/170910
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGao, Yen_US
dc.contributor.authorVanhoutte, PMen_US
dc.date.accessioned2012-10-30T06:11:24Z-
dc.date.available2012-10-30T06:11:24Z-
dc.date.issued1988en_US
dc.identifier.citationJournal Of Applied Physiology, 1988, v. 65 n. 6, p. 2400-2405en_US
dc.identifier.issn0161-7567en_US
dc.identifier.urihttp://hdl.handle.net/10722/170910-
dc.description.abstractExperiments were designed to determine whether the airway epithelium affects the membrane potential of the underlying smooth muscle. The effect of epithelium removal (by gentle rubbing) on the responsiveness of isolated canine bronchi was studied. Simultaneous recordings of mechanical and electrical activity were made in paired circumferential strips (with and without epithelium) of third-order bronchi. Changes in tension were recorded with a force transducer, and changes in membrane potential were measured with a microelectrode. The cell membrane potential and resting tension of the bronchial smooth muscle were stable over a 150-min period and were not affected by removal of the epithelium. In the presence of antagonists at muscarinic and adrenergic receptors, the resting tension and membrane potential were comparable in preparations with and without epithelium. By contrast, the anticholinesterase, echothiophate, caused depolarization in bronchi without epithelium. Exposure to high potassium induced similar levels of depolarization and contraction in tissues with and without epithelium. No significant differences in threshold for depolarization or for mechanical activation in the membrane potential-tension relationship were noted in the presence or absence of epithelium. In the presence of echothiophate, removal of the epithelium augmented the contraction of the bronchi to acetylcholine; the depolarization of the cell membrane induced by the cholinergic transmitter was significantly larger than in control tissues, even when matched contractions were compared. These observations indicate that the respiratory epithelium generates an inhibitory substance that dampens depolarization and contraction of bronchial smooth muscle caused by acetylcholine.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBronchi - Drug Effects - Physiologyen_US
dc.subject.meshDogsen_US
dc.subject.meshEpithelium - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentials - Drug Effectsen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshPotassium - Pharmacologyen_US
dc.subject.meshRespiratory Muscles - Drug Effects - Physiologyen_US
dc.titleRemoval of the epithelium potentiates acetylcholine in depolarizing canine bronchial smooth muscleen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3215839-
dc.identifier.scopuseid_2-s2.0-0024242332en_US
dc.identifier.volume65en_US
dc.identifier.issue6en_US
dc.identifier.spage2400en_US
dc.identifier.epage2405en_US
dc.identifier.isiWOS:A1988R498500006-
dc.identifier.scopusauthoridGao, Y=7404706442en_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US

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