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Article: Alterations of lysophosphatidylcholine levels of canine heart: Modes of extraction and storage

TitleAlterations of lysophosphatidylcholine levels of canine heart: Modes of extraction and storage
Authors
Issue Date1984
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio
Citation
Analytical Biochemistry, 1984, v. 137 n. 2, p. 277-281 How to Cite?
AbstractThe extraction of lysophosphatidylcholine from canine heart and the storage of lysophosphatidylcholine in total lipid extracts were investigated. The lysolipid was effectively extracted from canine heart by chloroform/methanol (1/2) and the maximum recovery of the lysolipid was 89-92%. Changes in levels of the lysolipid were observed when the tissue was stored at 0°C for 60 min or at -20°C for 7 days. An alteration in the lysophosphatidylcholine level was also observed when the lipid extract from the heart was stored in theoretical lower phase (chloroform/methanol/water; 86/14/1). In order to accurately assess the level of lysophosphatidylcholine in the canine heart, the lipid should be examined immediately, from fresh tissue and the lipid extract stored in chloroform/methanol (2/1) or without solvent under nitrogen prior to separation and determination.
Persistent Identifierhttp://hdl.handle.net/10722/170722
ISSN
2015 Impact Factor: 2.243
2015 SCImago Journal Rankings: 0.725
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMock, Ten_US
dc.contributor.authorPelletier, MPJen_US
dc.contributor.authorMan, RYKen_US
dc.contributor.authorChoy, PCen_US
dc.date.accessioned2012-10-30T06:10:35Z-
dc.date.available2012-10-30T06:10:35Z-
dc.date.issued1984en_US
dc.identifier.citationAnalytical Biochemistry, 1984, v. 137 n. 2, p. 277-281en_US
dc.identifier.issn0003-2697en_US
dc.identifier.urihttp://hdl.handle.net/10722/170722-
dc.description.abstractThe extraction of lysophosphatidylcholine from canine heart and the storage of lysophosphatidylcholine in total lipid extracts were investigated. The lysolipid was effectively extracted from canine heart by chloroform/methanol (1/2) and the maximum recovery of the lysolipid was 89-92%. Changes in levels of the lysolipid were observed when the tissue was stored at 0°C for 60 min or at -20°C for 7 days. An alteration in the lysophosphatidylcholine level was also observed when the lipid extract from the heart was stored in theoretical lower phase (chloroform/methanol/water; 86/14/1). In order to accurately assess the level of lysophosphatidylcholine in the canine heart, the lipid should be examined immediately, from fresh tissue and the lipid extract stored in chloroform/methanol (2/1) or without solvent under nitrogen prior to separation and determination.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabioen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshLysophosphatidylcholines - Isolation & Purificationen_US
dc.subject.meshMaleen_US
dc.subject.meshMyocardium - Analysisen_US
dc.subject.meshSolventsen_US
dc.subject.meshSpecimen Handlingen_US
dc.subject.meshTime Factorsen_US
dc.titleAlterations of lysophosphatidylcholine levels of canine heart: Modes of extraction and storageen_US
dc.typeArticleen_US
dc.identifier.emailMan, RYK:rykman@hkucc.hku.hken_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0003-2697(84)90085-X-
dc.identifier.pmid6731813-
dc.identifier.scopuseid_2-s2.0-0021142301en_US
dc.identifier.volume137en_US
dc.identifier.issue2en_US
dc.identifier.spage277en_US
dc.identifier.epage281en_US
dc.identifier.isiWOS:A1984SK77400002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMock, T=35954418100en_US
dc.identifier.scopusauthoridPelletier, MPJ=7102314760en_US
dc.identifier.scopusauthoridMan, RYK=7004986435en_US
dc.identifier.scopusauthoridChoy, PC=7006633002en_US

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