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Article: Calcium dependence of prejunctional inhibitory effects of adenosine and acetylcholine on adrenergic neurotransmission in canine saphenous veins

TitleCalcium dependence of prejunctional inhibitory effects of adenosine and acetylcholine on adrenergic neurotransmission in canine saphenous veins
Authors
Issue Date1981
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 1981, v. 72 n. 2-3, p. 189-198 How to Cite?
AbstractIn canine blood vessels acetylcholine and adenosine inhibit the exocytotic release of norepinephrine during nerve stimulation. The present experiments were designed to determine the Ca2+ dependence of these prejunctional effects. Segments of canine saphenous veins were mounted for isometric tension recording in organ chambers filled with Krebs-Ringer or Tyrode solution. Altering the Ca2+ concentration of the solution did not affect the inhibitory response to acetylcholine during nerve stimulation; the prejunctional potency of adenosine was inversely related to the Ca2+ concentration of the bath content. The ionophore A23187 caused contractions which were inhibited by phentolamine, verapamil, and adenosine but were augmented by acetylcholine. Helical strips of dog saphenous veins were incubated in [3H]norepinephrine and mounted for superfusion and determination of [3H]norepinephrine in the superfusate. A23187 increased the overflow of [3H]norepinephrine. Acetylcholine augmented this efflux; by contrast adenosine decreased the release induced by the ionophore. The results demonstrate that the prejunctional effect of acetylcholine was not due to direct interference with the availability of Ca2+ for the electro-secretory process in adrenergic nerve terminals and suggest that adenosine interferes either with the coupling role of the activator ion or its extrusion from the neuroplasm.
Persistent Identifierhttp://hdl.handle.net/10722/170643
ISSN
2015 Impact Factor: 2.73
2015 SCImago Journal Rankings: 1.115
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorCollis, MGen_US
dc.contributor.authorJanssens, WJen_US
dc.contributor.authorVerbeuren, TJen_US
dc.date.accessioned2012-10-30T06:10:16Z-
dc.date.available2012-10-30T06:10:16Z-
dc.date.issued1981en_US
dc.identifier.citationEuropean Journal Of Pharmacology, 1981, v. 72 n. 2-3, p. 189-198en_US
dc.identifier.issn0014-2999en_US
dc.identifier.urihttp://hdl.handle.net/10722/170643-
dc.description.abstractIn canine blood vessels acetylcholine and adenosine inhibit the exocytotic release of norepinephrine during nerve stimulation. The present experiments were designed to determine the Ca2+ dependence of these prejunctional effects. Segments of canine saphenous veins were mounted for isometric tension recording in organ chambers filled with Krebs-Ringer or Tyrode solution. Altering the Ca2+ concentration of the solution did not affect the inhibitory response to acetylcholine during nerve stimulation; the prejunctional potency of adenosine was inversely related to the Ca2+ concentration of the bath content. The ionophore A23187 caused contractions which were inhibited by phentolamine, verapamil, and adenosine but were augmented by acetylcholine. Helical strips of dog saphenous veins were incubated in [3H]norepinephrine and mounted for superfusion and determination of [3H]norepinephrine in the superfusate. A23187 increased the overflow of [3H]norepinephrine. Acetylcholine augmented this efflux; by contrast adenosine decreased the release induced by the ionophore. The results demonstrate that the prejunctional effect of acetylcholine was not due to direct interference with the availability of Ca2+ for the electro-secretory process in adrenergic nerve terminals and suggest that adenosine interferes either with the coupling role of the activator ion or its extrusion from the neuroplasm.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_US
dc.relation.ispartofEuropean Journal of Pharmacologyen_US
dc.subject.meshAcetylcholine - Physiologyen_US
dc.subject.meshAdenosine - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Physiologyen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contractionen_US
dc.subject.meshMuscle, Smooth, Vascular - Physiologyen_US
dc.subject.meshNorepinephrine - Metabolismen_US
dc.subject.meshSaphenous Vein - Innervationen_US
dc.subject.meshSympathetic Nervous System - Physiologyen_US
dc.subject.meshSynaptic Transmissionen_US
dc.titleCalcium dependence of prejunctional inhibitory effects of adenosine and acetylcholine on adrenergic neurotransmission in canine saphenous veinsen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0014-2999(81)90273-9-
dc.identifier.pmid6265245-
dc.identifier.scopuseid_2-s2.0-0019467003en_US
dc.identifier.volume72en_US
dc.identifier.issue2-3en_US
dc.identifier.spage189en_US
dc.identifier.epage198en_US
dc.identifier.isiWOS:A1981LX35700005-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridCollis, MG=7005797278en_US
dc.identifier.scopusauthoridJanssens, WJ=7006876881en_US
dc.identifier.scopusauthoridVerbeuren, TJ=7007006534en_US

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