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Article: Evoked release of endogenous norepinephrine in the canine saphenous vein. Inhibition by acetylcholine

TitleEvoked release of endogenous norepinephrine in the canine saphenous vein. Inhibition by acetylcholine
Authors
Issue Date1979
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://circres.ahajournals.org
Citation
Circulation Research, 1979, v. 45 n. 5, p. 608-614 How to Cite?
AbstractMost of the information concerning adrenergic neurotransmission in the blood vessel wall has been obtained from isolated blood vessels previously incubated with radiolabeled norepinephrine. In the present study, we determined the tissue content of norepinephrine and dopamine in the dog's saphenous vein using a radioenzymatic assay; tissue content of dopamine was 30 times less than tissue content of norepinephrine, and no epinephrine could be detected. During superfusion of isolated canine saphenous vein preparations, superfusate samples were collected for subsequent radioenzymatic analysis of norepinephrine, epinephrine, and dopamine. The basal efflux of endogenous norepinephrine declined slightly with time. Nerve stimulation caused frequency-dependent increases in tension, paralleled by increase in efflux of endogenous norepinephrine. The changes in tension were correlated significantly with the changes in norepinephrine overflow. Acetylcholine at 5x10-7M had no effect on basal tension or basal norepinephrine overflow. During nerve stimulation at 2 Hz and at 5 Hz, it significantly depressed the contractile response and the evoked overflow of endogenous norepinephrine. Neither dopamine nor epinephrine was detected during these superfusion studies. The present experiments introduce the means for measuring endogenous overflow in the canine saphenous vein, validate earlier work on this blood vessel, and, in particular, provide direct evidence for the inhibitory effect of acetylcholine on adrenergic neurotransmission in the blood vessel wall.
Persistent Identifierhttp://hdl.handle.net/10722/170590
ISSN
2015 Impact Factor: 11.551
2015 SCImago Journal Rankings: 5.755
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorCoen, EPen_US
dc.contributor.authorDe Ridder, WJen_US
dc.contributor.authorVerbeuren, TJen_US
dc.date.accessioned2012-10-30T06:10:03Z-
dc.date.available2012-10-30T06:10:03Z-
dc.date.issued1979en_US
dc.identifier.citationCirculation Research, 1979, v. 45 n. 5, p. 608-614en_US
dc.identifier.issn0009-7330en_US
dc.identifier.urihttp://hdl.handle.net/10722/170590-
dc.description.abstractMost of the information concerning adrenergic neurotransmission in the blood vessel wall has been obtained from isolated blood vessels previously incubated with radiolabeled norepinephrine. In the present study, we determined the tissue content of norepinephrine and dopamine in the dog's saphenous vein using a radioenzymatic assay; tissue content of dopamine was 30 times less than tissue content of norepinephrine, and no epinephrine could be detected. During superfusion of isolated canine saphenous vein preparations, superfusate samples were collected for subsequent radioenzymatic analysis of norepinephrine, epinephrine, and dopamine. The basal efflux of endogenous norepinephrine declined slightly with time. Nerve stimulation caused frequency-dependent increases in tension, paralleled by increase in efflux of endogenous norepinephrine. The changes in tension were correlated significantly with the changes in norepinephrine overflow. Acetylcholine at 5x10-7M had no effect on basal tension or basal norepinephrine overflow. During nerve stimulation at 2 Hz and at 5 Hz, it significantly depressed the contractile response and the evoked overflow of endogenous norepinephrine. Neither dopamine nor epinephrine was detected during these superfusion studies. The present experiments introduce the means for measuring endogenous overflow in the canine saphenous vein, validate earlier work on this blood vessel, and, in particular, provide direct evidence for the inhibitory effect of acetylcholine on adrenergic neurotransmission in the blood vessel wall.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://circres.ahajournals.orgen_US
dc.relation.ispartofCirculation Researchen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCatechol O-Methyltransferaseen_US
dc.subject.meshCatecholaminesen_US
dc.subject.meshClinical Enzyme Testsen_US
dc.subject.meshDogsen_US
dc.subject.meshDopamineen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshEpinephrineen_US
dc.subject.meshEvoked Potentialsen_US
dc.subject.meshNorepinephrine - Metabolismen_US
dc.subject.meshSaphenous Vein - Innervation - Physiologyen_US
dc.titleEvoked release of endogenous norepinephrine in the canine saphenous vein. Inhibition by acetylcholineen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid487523-
dc.identifier.scopuseid_2-s2.0-0018600094en_US
dc.identifier.volume45en_US
dc.identifier.issue5en_US
dc.identifier.spage608en_US
dc.identifier.epage614en_US
dc.identifier.isiWOS:A1979HS05400005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridCoen, EP=35501191200en_US
dc.identifier.scopusauthoridDe Ridder, WJ=6602324543en_US
dc.identifier.scopusauthoridVerbeuren, TJ=7007006534en_US

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