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Article: Inhibition of norepinephrine 3H release from sympathetic nerve endings in veins by acetylcholine

TitleInhibition of norepinephrine 3H release from sympathetic nerve endings in veins by acetylcholine
Authors
Issue Date1973
PublisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org
Citation
Journal Of Pharmacology And Experimental Therapeutics, 1973, v. 185 n. 2, p. 386-394 How to Cite?
AbstractExperiments were designed to determine if the relaxation obtained with acetylcholine during electric stimulation of cutaneous veins results from inhibition of norepinephrine release. Helical strips of dog saphenous veins were incubated (4 hr) in Krebs Ringer solution which contained norepinephrine-7-3H (5 x 10-8 g/ml). The preparations were then rinsed and mounted for superfusion (3 ml/min) and isometric tension recording. The superfusate was collected at timed intervals for estimation of total radioactivity or for column chromatographic separation of norepinephrine and its metabolites. Electric stimulation increased the tension and the total radioactivity of the superfusate; it increased the amount of intact norepinephrine-3H present more than the metabolite fractions. Acetylcholine (5 x 10-8 to 5 x 10-7 g/ml) depressed the contractions and diminished the norepinephrine-3H efflux. Isoproterenol depressed the contractions but did not affect the efflux of radioactivity. Tyramine (2 x 10-6 g/ml) augmented both tension and efflux of radioactivity, but these actions were not depressed by acetylcholine. These experiments show that acetylcholine causes relaxation of venous smooth muscle constricted by sympathetic stimulation and does so by inhibiting the release of norepinephrine from the nerve endings. By contrast, acetylcholine does not inhibit the release of norepinephrine by tyramine.
Persistent Identifierhttp://hdl.handle.net/10722/170499
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.847
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorLorenz, RRen_US
dc.contributor.authorTyce, GMen_US
dc.date.accessioned2012-10-30T06:09:43Z-
dc.date.available2012-10-30T06:09:43Z-
dc.date.issued1973en_US
dc.identifier.citationJournal Of Pharmacology And Experimental Therapeutics, 1973, v. 185 n. 2, p. 386-394en_US
dc.identifier.issn0022-3565en_US
dc.identifier.urihttp://hdl.handle.net/10722/170499-
dc.description.abstractExperiments were designed to determine if the relaxation obtained with acetylcholine during electric stimulation of cutaneous veins results from inhibition of norepinephrine release. Helical strips of dog saphenous veins were incubated (4 hr) in Krebs Ringer solution which contained norepinephrine-7-3H (5 x 10-8 g/ml). The preparations were then rinsed and mounted for superfusion (3 ml/min) and isometric tension recording. The superfusate was collected at timed intervals for estimation of total radioactivity or for column chromatographic separation of norepinephrine and its metabolites. Electric stimulation increased the tension and the total radioactivity of the superfusate; it increased the amount of intact norepinephrine-3H present more than the metabolite fractions. Acetylcholine (5 x 10-8 to 5 x 10-7 g/ml) depressed the contractions and diminished the norepinephrine-3H efflux. Isoproterenol depressed the contractions but did not affect the efflux of radioactivity. Tyramine (2 x 10-6 g/ml) augmented both tension and efflux of radioactivity, but these actions were not depressed by acetylcholine. These experiments show that acetylcholine causes relaxation of venous smooth muscle constricted by sympathetic stimulation and does so by inhibiting the release of norepinephrine from the nerve endings. By contrast, acetylcholine does not inhibit the release of norepinephrine by tyramine.en_US
dc.languageengen_US
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.orgen_US
dc.relation.ispartofJournal of Pharmacology and Experimental Therapeuticsen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshDogsen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshIsoproterenol - Pharmacologyen_US
dc.subject.meshMuscle, Smooth - Innervation - Metabolismen_US
dc.subject.meshNerve Endings - Metabolismen_US
dc.subject.meshNorepinephrine - Antagonists & Inhibitors - Isolation & Purification - Metabolismen_US
dc.subject.meshSaphenous Vein - Innervationen_US
dc.subject.meshSympathetic Nervous System - Metabolismen_US
dc.subject.meshTritiumen_US
dc.subject.meshTyramine - Pharmacologyen_US
dc.titleInhibition of norepinephrine 3H release from sympathetic nerve endings in veins by acetylcholineen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, PM:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid4703828-
dc.identifier.scopuseid_2-s2.0-0015764585en_US
dc.identifier.volume185en_US
dc.identifier.issue2en_US
dc.identifier.spage386en_US
dc.identifier.epage394en_US
dc.identifier.isiWOS:A1973P985800023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_US
dc.identifier.scopusauthoridLorenz, RR=7402095192en_US
dc.identifier.scopusauthoridTyce, GM=7004909546en_US

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