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Article: Activation of lytic cycle of Epstein-Barr virus by suberoylanilide hydroxamic acid leads to apoptosis and tumor growth suppression of nasopharyngeal carcinoma

TitleActivation of lytic cycle of Epstein-Barr virus by suberoylanilide hydroxamic acid leads to apoptosis and tumor growth suppression of nasopharyngeal carcinoma
Authors
KeywordsApoptosis
Epstein-Barr Virus
Lytic Cycle
Nasopharyngeal Carcinoma
Suberoylanilide Hydroxamic Acid
Issue Date2012
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2012, v. 131 n. 8, p. 1930-1940 How to Cite?
AbstractNasopharyngeal carcinoma (NPC) is strongly associated with Epstein-Barr virus (EBV). We reported that suberoylanilide hydroxamic acid (SAHA) induced EBV lytic cycle in EBV-positive gastric carcinoma cells and mediated enhanced cell death. However, expression of EBV lytic proteins was thought to exert antiapoptotic effect in EBV-infected cells. Here, we examined the in vitro and in vivo effects of SAHA on EBV lytic cycle induction in NPC cells and investigated the cellular consequences. Micromolar concentrations of SAHA significantly induced EBV lytic cycle in EBV-positive NPC cells. Increased apoptosis and proteolytic cleavage of poly(ADP-ribose) polymerase and caspase-3, -7 and -9 in EBV-positive versus EBV-negative NPC cells were observed. More than 85% of NPC cells expressing immediate-early (Zta), early (BMRF1) or late (gp350/220) lytic proteins coexpressed cleaved caspase-3. Tracking of expression of EBV lytic proteins and cleaved caspase-3 over time demonstrated that NPC cells proceeded to apoptosis following EBV lytic cycle induction. Inhibition of EBV DNA replication and late lytic protein expression by phosphonoformic acid did not impact on SAHA's induced cell death in NPC, indicating that early rather than late phase of EBV lytic cycle contributed to the apoptotic effect. In vivo effects of SAHA on EBV lytic cycle induction and tumor growth suppression were also observed in NPC xenografts in nude mice. Taken together, our data indicated that activation of lytic cycle from latent cycle of EBV by SAHA leads to apoptosis and tumor growth suppression of NPC thereby providing experimental evidence for virus-targeted therapy against EBV-positive cancer. © 2012 UICC.
Persistent Identifierhttp://hdl.handle.net/10722/170464
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHui, KFen_US
dc.contributor.authorHo, DNen_US
dc.contributor.authorTsang, Cen_US
dc.contributor.authorMiddeldorp, JMen_US
dc.contributor.authorTsao, GSen_US
dc.contributor.authorChiang, AKen_US
dc.date.accessioned2012-10-30T06:09:10Z-
dc.date.available2012-10-30T06:09:10Z-
dc.date.issued2012en_US
dc.identifier.citationInternational Journal Of Cancer, 2012, v. 131 n. 8, p. 1930-1940en_US
dc.identifier.issn0020-7136en_US
dc.identifier.urihttp://hdl.handle.net/10722/170464-
dc.description.abstractNasopharyngeal carcinoma (NPC) is strongly associated with Epstein-Barr virus (EBV). We reported that suberoylanilide hydroxamic acid (SAHA) induced EBV lytic cycle in EBV-positive gastric carcinoma cells and mediated enhanced cell death. However, expression of EBV lytic proteins was thought to exert antiapoptotic effect in EBV-infected cells. Here, we examined the in vitro and in vivo effects of SAHA on EBV lytic cycle induction in NPC cells and investigated the cellular consequences. Micromolar concentrations of SAHA significantly induced EBV lytic cycle in EBV-positive NPC cells. Increased apoptosis and proteolytic cleavage of poly(ADP-ribose) polymerase and caspase-3, -7 and -9 in EBV-positive versus EBV-negative NPC cells were observed. More than 85% of NPC cells expressing immediate-early (Zta), early (BMRF1) or late (gp350/220) lytic proteins coexpressed cleaved caspase-3. Tracking of expression of EBV lytic proteins and cleaved caspase-3 over time demonstrated that NPC cells proceeded to apoptosis following EBV lytic cycle induction. Inhibition of EBV DNA replication and late lytic protein expression by phosphonoformic acid did not impact on SAHA's induced cell death in NPC, indicating that early rather than late phase of EBV lytic cycle contributed to the apoptotic effect. In vivo effects of SAHA on EBV lytic cycle induction and tumor growth suppression were also observed in NPC xenografts in nude mice. Taken together, our data indicated that activation of lytic cycle from latent cycle of EBV by SAHA leads to apoptosis and tumor growth suppression of NPC thereby providing experimental evidence for virus-targeted therapy against EBV-positive cancer. © 2012 UICC.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_US
dc.relation.ispartofInternational Journal of Canceren_US
dc.subjectApoptosisen_US
dc.subjectEpstein-Barr Virusen_US
dc.subjectLytic Cycleen_US
dc.subjectNasopharyngeal Carcinomaen_US
dc.subjectSuberoylanilide Hydroxamic Aciden_US
dc.titleActivation of lytic cycle of Epstein-Barr virus by suberoylanilide hydroxamic acid leads to apoptosis and tumor growth suppression of nasopharyngeal carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailChiang, AK:chiangak@hkucc.hku.hken_US
dc.identifier.authorityChiang, AK=rp00403en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ijc.27439en_US
dc.identifier.pmid22261816-
dc.identifier.scopuseid_2-s2.0-84865308897en_US
dc.identifier.hkuros200679-
dc.identifier.hkuros221261-
dc.identifier.eissn1097-0215-
dc.identifier.isiWOS:000307893400021-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHui, KF=35848529600en_US
dc.identifier.scopusauthoridHo, DN=55066321300en_US
dc.identifier.scopusauthoridTsang, C=24831236400en_US
dc.identifier.scopusauthoridMiddeldorp, JM=35493462100en_US
dc.identifier.scopusauthoridTsao, GS=55068792800en_US
dc.identifier.scopusauthoridChiang, AK=7101623534en_US

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