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Article: Identification of genes expressed during myocardial development
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TitleIdentification of genes expressed during myocardial development
 
AuthorsChan, SY1
Chan, AKW1
Cheung, BPK1
Liang, Y1
Leung, MP1
 
Issue Date2003
 
PublisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/
 
CitationChinese Medical Journal, 2003, v. 116 n. 9, p. 1329-1332 [How to Cite?]
 
AbstractObjective. To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation. Methods. mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed. Results. Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts. Conclusions. We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.
 
ISSN0366-6999
2013 Impact Factor: 1.016
2013 SCImago Journal Rankings: 0.433
 
ISI Accession Number IDWOS:000185645900011
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, SY
 
dc.contributor.authorChan, AKW
 
dc.contributor.authorCheung, BPK
 
dc.contributor.authorLiang, Y
 
dc.contributor.authorLeung, MP
 
dc.date.accessioned2012-10-30T06:07:32Z
 
dc.date.available2012-10-30T06:07:32Z
 
dc.date.issued2003
 
dc.description.abstractObjective. To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation. Methods. mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed. Results. Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts. Conclusions. We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationChinese Medical Journal, 2003, v. 116 n. 9, p. 1329-1332 [How to Cite?]
 
dc.identifier.epage1332
 
dc.identifier.hkuros84264
 
dc.identifier.isiWOS:000185645900011
 
dc.identifier.issn0366-6999
2013 Impact Factor: 1.016
2013 SCImago Journal Rankings: 0.433
 
dc.identifier.issue9
 
dc.identifier.pmid14527359
 
dc.identifier.scopuseid_2-s2.0-0141503335
 
dc.identifier.spage1329
 
dc.identifier.urihttp://hdl.handle.net/10722/170332
 
dc.identifier.volume116
 
dc.languageeng
 
dc.publisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/
 
dc.publisher.placeChina
 
dc.relation.ispartofChinese Medical Journal
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAged
 
dc.subject.meshCells, Cultured
 
dc.subject.meshCollagen
 
dc.subject.meshCollagen Type I
 
dc.subject.meshCollagen Type Iii - Genetics
 
dc.subject.meshDna-Binding Proteins - Genetics
 
dc.subject.meshForkhead Transcription Factors
 
dc.subject.meshGene Expression - Physiology
 
dc.subject.meshHeart - Embryology - Growth & Development
 
dc.subject.meshHeterogeneous-Nuclear Ribonucleoproteins - Genetics
 
dc.subject.meshHumans
 
dc.subject.meshNerve Tissue Proteins - Genetics
 
dc.subject.meshNucleic Acid Hybridization
 
dc.subject.meshRna-Binding Proteins
 
dc.subject.meshTranscription Factors
 
dc.subject.meshTransforming Growth Factor Beta - Genetics
 
dc.subject.meshTransforming Growth Factor Beta1
 
dc.subject.meshVinculin - Genetics
 
dc.titleIdentification of genes expressed during myocardial development
 
dc.typeArticle
 
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<item><contributor.author>Chan, SY</contributor.author>
<contributor.author>Chan, AKW</contributor.author>
<contributor.author>Cheung, BPK</contributor.author>
<contributor.author>Liang, Y</contributor.author>
<contributor.author>Leung, MP</contributor.author>
<date.accessioned>2012-10-30T06:07:32Z</date.accessioned>
<date.available>2012-10-30T06:07:32Z</date.available>
<date.issued>2003</date.issued>
<identifier.citation>Chinese Medical Journal, 2003, v. 116 n. 9, p. 1329-1332</identifier.citation>
<identifier.issn>0366-6999</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/170332</identifier.uri>
<description.abstract>Objective. To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation. Methods. mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed. Results. Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts. Conclusions. We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.</description.abstract>
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<subject.mesh>Aged</subject.mesh>
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<subject.mesh>Collagen Type I</subject.mesh>
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Author Affiliations
  1. The University of Hong Kong