Article: Identification of genes expressed during myocardial development

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TitleIdentification of genes expressed during myocardial development
AuthorsChan, SY1
Chan, AKW1
Cheung, BPK1
Liang, Y1
Leung, MP1
Issue Date2003
PublisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/
CitationChinese Medical Journal, 2003, v. 116 n. 9, p. 1329-1332 [How to Cite?]
AbstractObjective. To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation. Methods. mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed. Results. Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts. Conclusions. We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.
ISSN0366-6999
2011 Impact Factor: 0.864
2011 SCImago Journal Rankings: 0.077
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChan, SY
dc.contributor.authorChan, AKW
dc.contributor.authorCheung, BPK
dc.contributor.authorLiang, Y
dc.contributor.authorLeung, MP
dc.date.accessioned2012-10-30T06:07:32Z
dc.date.available2012-10-30T06:07:32Z
dc.date.issued2003
dc.description.abstractObjective. To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation. Methods. mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed. Results. Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts. Conclusions. We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationChinese Medical Journal, 2003, v. 116 n. 9, p. 1329-1332 [How to Cite?]
dc.identifier.epage1332
dc.identifier.hkuros84264
dc.identifier.issn0366-6999
2011 Impact Factor: 0.864
2011 SCImago Journal Rankings: 0.077
dc.identifier.issue9
dc.identifier.pmid14527359
dc.identifier.scopuseid_2-s2.0-0141503335
dc.identifier.spage1329
dc.identifier.urihttp://hdl.handle.net/10722/170332
dc.identifier.volume116
dc.languageeng
dc.publisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/
dc.publisher.placeChina
dc.relation.ispartofChinese Medical Journal
dc.relation.referencesReferences in Scopus
dc.subject.meshAged
dc.subject.meshCells, Cultured
dc.subject.meshCollagen
dc.subject.meshCollagen Type I
dc.subject.meshCollagen Type Iii - Genetics
dc.subject.meshDna-Binding Proteins - Genetics
dc.subject.meshForkhead Transcription Factors
dc.subject.meshGene Expression - Physiology
dc.subject.meshHeart - Embryology - Growth & Development
dc.subject.meshHeterogeneous-Nuclear Ribonucleoproteins - Genetics
dc.subject.meshHumans
dc.subject.meshNerve Tissue Proteins - Genetics
dc.subject.meshNucleic Acid Hybridization
dc.subject.meshRna-Binding Proteins
dc.subject.meshTranscription Factors
dc.subject.meshTransforming Growth Factor Beta - Genetics
dc.subject.meshTransforming Growth Factor Beta1
dc.subject.meshVinculin - Genetics
dc.titleIdentification of genes expressed during myocardial development
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong