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Article: Identification of genes expressed during myocardial development

TitleIdentification of genes expressed during myocardial development
Authors
Issue Date2003
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2003, v. 116 n. 9, p. 1329-1332 How to Cite?
Abstract
Objective. To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation. Methods. mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed. Results. Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts. Conclusions. We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.
DescriptionFulltext link: http://www.ecmj.org.cn/ch/reader/view_abstract.aspx?volume=116&issue=9&start_page=1329
Persistent Identifierhttp://hdl.handle.net/10722/170332
ISSN
2013 Impact Factor: 1.016
2013 SCImago Journal Rankings: 0.433
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChan, SYen_US
dc.contributor.authorChan, AKWen_US
dc.contributor.authorCheung, BPKen_US
dc.contributor.authorLiang, Yen_US
dc.contributor.authorLeung, MPen_US
dc.date.accessioned2012-10-30T06:07:32Z-
dc.date.available2012-10-30T06:07:32Z-
dc.date.issued2003en_US
dc.identifier.citationChinese Medical Journal, 2003, v. 116 n. 9, p. 1329-1332en_US
dc.identifier.issn0366-6999en_US
dc.identifier.urihttp://hdl.handle.net/10722/170332-
dc.descriptionFulltext link: http://www.ecmj.org.cn/ch/reader/view_abstract.aspx?volume=116&issue=9&start_page=1329-
dc.description.abstractObjective. To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation. Methods. mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed. Results. Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts. Conclusions. We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.en_US
dc.languageengen_US
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_US
dc.relation.ispartofChinese Medical Journalen_US
dc.subject.meshAgeden_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCollagenen_US
dc.subject.meshCollagen Type Ien_US
dc.subject.meshCollagen Type Iii - Geneticsen_US
dc.subject.meshDna-Binding Proteins - Geneticsen_US
dc.subject.meshForkhead Transcription Factorsen_US
dc.subject.meshGene Expression - Physiologyen_US
dc.subject.meshHeart - Embryology - Growth & Developmenten_US
dc.subject.meshHeterogeneous-Nuclear Ribonucleoproteins - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshNerve Tissue Proteins - Geneticsen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna-Binding Proteinsen_US
dc.subject.meshTranscription Factorsen_US
dc.subject.meshTransforming Growth Factor Beta - Geneticsen_US
dc.subject.meshTransforming Growth Factor Beta1en_US
dc.subject.meshVinculin - Geneticsen_US
dc.titleIdentification of genes expressed during myocardial developmenten_US
dc.typeArticleen_US
dc.identifier.emailChan, SY:sychan@hkucc.hku.hken_US
dc.identifier.emailChan, KW: achankw@graduate.hku.hk-
dc.identifier.emailCheung, BPK: bpkcheun@HKUCC.hku.hk-
dc.identifier.emailLeung, MP: mpleung@hkucc.hku.hk-
dc.identifier.authorityChan, SY=rp00356en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid14527359en_US
dc.identifier.scopuseid_2-s2.0-0141503335en_US
dc.identifier.hkuros84264-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141503335&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume116en_US
dc.identifier.issue9en_US
dc.identifier.spage1329en_US
dc.identifier.epage1332en_US
dc.identifier.isiWOS:000185645900011-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridChan, SY=7404255082en_US
dc.identifier.scopusauthoridChan, AKW=37019606100en_US
dc.identifier.scopusauthoridCheung, BPK=7103294773en_US
dc.identifier.scopusauthoridLiang, Y=35778546100en_US
dc.identifier.scopusauthoridLeung, MP=7201944800en_US

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