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Article: Expression of full length or truncated epidermal growth factor precursor transforms NIH3T3 fibroblasts.

TitleExpression of full length or truncated epidermal growth factor precursor transforms NIH3T3 fibroblasts.
Authors
Issue Date1999
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 1999, v. 15 n. 2, p. 281-284 How to Cite?
AbstractEpidermal growth factor (EGF) is derived from a large precursor (EGFP) of unusual structure. As EGFP remains unprocessed in certain tissues, it is of biological relevance to study its activity. Activation of the EGF receptor by EGF is involved in transformation of NIH3T3 fibroblasts. We isolated clones of NIH3T3 expressing full length, cytoplasmic region deleted or EGF-repeats deleted EGFP. All clones formed colonies in soft agarose and tumors in nude mice. Two clones expressing EGF-repeats deleted EGFP formed more and larger colonies. To conclude, EGFP is biologically active. Deletion of the 8 EGF repeats may enhance anchorage independent growth in NIH3T3.
Persistent Identifierhttp://hdl.handle.net/10722/170299
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwan, RWen_US
dc.contributor.authorWong, RWen_US
dc.contributor.authorChan, SYen_US
dc.date.accessioned2012-10-30T06:07:19Z-
dc.date.available2012-10-30T06:07:19Z-
dc.date.issued1999en_US
dc.identifier.citationInternational Journal Of Oncology, 1999, v. 15 n. 2, p. 281-284en_US
dc.identifier.issn1019-6439en_US
dc.identifier.urihttp://hdl.handle.net/10722/170299-
dc.description.abstractEpidermal growth factor (EGF) is derived from a large precursor (EGFP) of unusual structure. As EGFP remains unprocessed in certain tissues, it is of biological relevance to study its activity. Activation of the EGF receptor by EGF is involved in transformation of NIH3T3 fibroblasts. We isolated clones of NIH3T3 expressing full length, cytoplasmic region deleted or EGF-repeats deleted EGFP. All clones formed colonies in soft agarose and tumors in nude mice. Two clones expressing EGF-repeats deleted EGFP formed more and larger colonies. To conclude, EGFP is biologically active. Deletion of the 8 EGF repeats may enhance anchorage independent growth in NIH3T3.en_US
dc.languageengen_US
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_US
dc.relation.ispartofInternational journal of oncologyen_US
dc.subject.mesh3T3 Cellsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Line, Transformeden_US
dc.subject.meshClone Cells - Drug Effectsen_US
dc.subject.meshEpidermal Growth Factor - Toxicityen_US
dc.subject.meshFibroblasts - Drug Effectsen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshProtein Precursors - Toxicityen_US
dc.subject.meshReceptor, Epidermal Growth Factor - Drug Effectsen_US
dc.subject.meshSignal Transduction - Drug Effectsen_US
dc.titleExpression of full length or truncated epidermal growth factor precursor transforms NIH3T3 fibroblasts.en_US
dc.typeArticleen_US
dc.identifier.emailChan, SY:sychan@hkucc.hku.hken_US
dc.identifier.authorityChan, SY=rp00356en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10402238-
dc.identifier.scopuseid_2-s2.0-0033174415en_US
dc.identifier.hkuros42099-
dc.identifier.volume15en_US
dc.identifier.issue2en_US
dc.identifier.spage281en_US
dc.identifier.epage284en_US
dc.identifier.isiWOS:000081469400011-
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridKwan, RW=7004066556en_US
dc.identifier.scopusauthoridWong, RW=16945028500en_US
dc.identifier.scopusauthoridChan, SY=7404255082en_US

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