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Article: Interleukin-12 induces interferon-γ expression and natural killer cytotoxicity in cord blood mononuclear cells

TitleInterleukin-12 induces interferon-γ expression and natural killer cytotoxicity in cord blood mononuclear cells
Authors
Issue Date1996
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
Citation
Pediatric Research, 1996, v. 39 n. 1, p. 150-155 How to Cite?
AbstractSevere viral infection in newborns has been attributed to immaturity of the immune system including a defect in natural killer cytotoxicity (NKC) and decreased production of cytokines that are important for natural killer (NK) function. We investigated the induction of interferon (IFN)-γ and activation of NK activity in adult and cord blood mononuclear cells (BMC) after IL-12 treatment. The levels of mRNA in these BMC were measured by Northern blot and reverse transcription polymerase chain reactions using primers specific for IFN-γ. The levels of IFN-γ protein were measured by ELISA. In the absence of IL-12, only adult BMC spontaneously produced low levels of IFN-γ. After IL-12 treatment, induction of IFN-γ expression was detected as early as 4 h in both cord and adult BMC. Both cord and adult cells showed similar levels of IFN-γ mRNA and protein expression in response to IL-12 at a concentration as low as 10 U/mL. In contrast, upon phorbol ester and ionomycin treatment, adult BMC produced more IFN-γ mRNA than cord BMC. In a 51Cr release assay with human immunodeficiency-infected H9 cells as indicators, both cord and adult cells responded to IL-12 induction of NKC. Our findings demonstrate that cord BMC are capable of responding to IL-12 stimulation, competent in synthesizing IFN-γ, and able to mount NKC. Thus, it appears that the deficiency in IFN-γ production or NKC in cord cells is not due to an inherent defect in IL-12 response of the cord cells.
Persistent Identifierhttp://hdl.handle.net/10722/170283
ISSN
2015 Impact Factor: 2.761
2015 SCImago Journal Rankings: 1.290
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, ASen_US
dc.contributor.authorSigaroudinia, Men_US
dc.contributor.authorYeung, MCen_US
dc.contributor.authorKohl, Sen_US
dc.date.accessioned2012-10-30T06:07:13Z-
dc.date.available2012-10-30T06:07:13Z-
dc.date.issued1996en_US
dc.identifier.citationPediatric Research, 1996, v. 39 n. 1, p. 150-155en_US
dc.identifier.issn0031-3998en_US
dc.identifier.urihttp://hdl.handle.net/10722/170283-
dc.description.abstractSevere viral infection in newborns has been attributed to immaturity of the immune system including a defect in natural killer cytotoxicity (NKC) and decreased production of cytokines that are important for natural killer (NK) function. We investigated the induction of interferon (IFN)-γ and activation of NK activity in adult and cord blood mononuclear cells (BMC) after IL-12 treatment. The levels of mRNA in these BMC were measured by Northern blot and reverse transcription polymerase chain reactions using primers specific for IFN-γ. The levels of IFN-γ protein were measured by ELISA. In the absence of IL-12, only adult BMC spontaneously produced low levels of IFN-γ. After IL-12 treatment, induction of IFN-γ expression was detected as early as 4 h in both cord and adult BMC. Both cord and adult cells showed similar levels of IFN-γ mRNA and protein expression in response to IL-12 at a concentration as low as 10 U/mL. In contrast, upon phorbol ester and ionomycin treatment, adult BMC produced more IFN-γ mRNA than cord BMC. In a 51Cr release assay with human immunodeficiency-infected H9 cells as indicators, both cord and adult cells responded to IL-12 induction of NKC. Our findings demonstrate that cord BMC are capable of responding to IL-12 stimulation, competent in synthesizing IFN-γ, and able to mount NKC. Thus, it appears that the deficiency in IFN-γ production or NKC in cord cells is not due to an inherent defect in IL-12 response of the cord cells.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/en_US
dc.relation.ispartofPediatric Researchen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCytotoxicity, Immunologicen_US
dc.subject.meshDna Primersen_US
dc.subject.meshFetal Blood - Immunologyen_US
dc.subject.meshHiv-1 - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-Gamma - Genetics - Metabolismen_US
dc.subject.meshInterleukin-12 - Pharmacologyen_US
dc.subject.meshIonomycin - Pharmacologyen_US
dc.subject.meshKiller Cells, Natural - Immunologyen_US
dc.subject.meshLeukocytes, Mononuclear - Immunologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhorbol Esters - Pharmacologyen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleInterleukin-12 induces interferon-γ expression and natural killer cytotoxicity in cord blood mononuclear cellsen_US
dc.typeArticleen_US
dc.identifier.emailLau, AS:asylau@hku.hken_US
dc.identifier.authorityLau, AS=rp00474en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1203/00006450-199601000-00023-
dc.identifier.pmid8825401-
dc.identifier.scopuseid_2-s2.0-0030064563en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030064563&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume39en_US
dc.identifier.issue1en_US
dc.identifier.spage150en_US
dc.identifier.epage155en_US
dc.identifier.isiWOS:A1996TM65800023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLau, AS=7202626202en_US
dc.identifier.scopusauthoridSigaroudinia, M=14122220300en_US
dc.identifier.scopusauthoridYeung, MC=7101861664en_US
dc.identifier.scopusauthoridKohl, S=7102175428en_US

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