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Article: Expression of insulin-like growth factor binding protein-4 (IGFBP-4) by rat neural cells - Comparison to other IGFBPs

TitleExpression of insulin-like growth factor binding protein-4 (IGFBP-4) by rat neural cells - Comparison to other IGFBPs
Authors
Issue Date1993
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpep
Citation
Regulatory Peptides, 1993, v. 48 n. 1-2, p. 123-132 How to Cite?
AbstractWe recently isolated and characterized the 24 kDa and N-glycosylated 28kDa insulin-like growth factor binding protein-4 (rIGFBP-4) from the B 104s rat neuronal cell line (Endocrinology, 129 (1991) 1009-1115). To examine the prevalence of IGFBP-4 secretion by cells of neural origin, we assessed the expression of IGFBP-4 in different neural cell types using ligand blotting, immunoblotting and blot hybridization with relevant cDNAs. A specific IGFBP-4 antibody raised against a synthetic 20 amino acid peptide was used for immunologic recognition. In all the neural cells tested (B104s, C6 astrocytoma, primary neonatal astrocytes and primary fetal neurons), IGFBP-4 was definitively identified by immunoblotting. Blot hybridization using a rat cDNA probe revealed expression of IGFBP-4 mRNA transcripts by all these cells. Using a combination of the same techniques, expression of IGFBP-1, -2, and -3 were also examined. The B 104s cells secreted primarily IGFBP-4; C6 cells secreted predominantly IGFBP-3 and small amount of IGFBP-4; both primary neonatal astrocytes and fetal neurons secreted IGFBP-2 as the major IGFBP accompanied by a small quantity of IGFBP-4. IGFBP-1 was not identified in any of the cell media. When probed with the respective IGFBP cDNAs, the mRNA abundance generally reflected the media IGFBP content. The expression of IGFBP-4 mRNA in vivo was examined as well and compared to that of IGFBP-1 and IGFBP-2. Transcripts for both IGFBP-2 and IGFBP-4 were found in all gross anatomical divisions of the rat brain from embryonic day 15 until adulthood, whereas IGFBP-1 was not detected at any time. IGFBP-4 mRNA tended to be more abundant at the youngest ages whereas IGFBP-2 increased during development. These data indicate that IGFBP-4 is produce by a variety of neural cell types and suggest that it may play a role in brain development.
Persistent Identifierhttp://hdl.handle.net/10722/170268
ISSN
2015 Impact Factor: 1.813
2015 SCImago Journal Rankings: 0.915
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChernausek, SDen_US
dc.contributor.authorMurray, MAen_US
dc.contributor.authorCheung, PTen_US
dc.date.accessioned2012-10-30T06:07:05Z-
dc.date.available2012-10-30T06:07:05Z-
dc.date.issued1993en_US
dc.identifier.citationRegulatory Peptides, 1993, v. 48 n. 1-2, p. 123-132en_US
dc.identifier.issn0167-0115en_US
dc.identifier.urihttp://hdl.handle.net/10722/170268-
dc.description.abstractWe recently isolated and characterized the 24 kDa and N-glycosylated 28kDa insulin-like growth factor binding protein-4 (rIGFBP-4) from the B 104s rat neuronal cell line (Endocrinology, 129 (1991) 1009-1115). To examine the prevalence of IGFBP-4 secretion by cells of neural origin, we assessed the expression of IGFBP-4 in different neural cell types using ligand blotting, immunoblotting and blot hybridization with relevant cDNAs. A specific IGFBP-4 antibody raised against a synthetic 20 amino acid peptide was used for immunologic recognition. In all the neural cells tested (B104s, C6 astrocytoma, primary neonatal astrocytes and primary fetal neurons), IGFBP-4 was definitively identified by immunoblotting. Blot hybridization using a rat cDNA probe revealed expression of IGFBP-4 mRNA transcripts by all these cells. Using a combination of the same techniques, expression of IGFBP-1, -2, and -3 were also examined. The B 104s cells secreted primarily IGFBP-4; C6 cells secreted predominantly IGFBP-3 and small amount of IGFBP-4; both primary neonatal astrocytes and fetal neurons secreted IGFBP-2 as the major IGFBP accompanied by a small quantity of IGFBP-4. IGFBP-1 was not identified in any of the cell media. When probed with the respective IGFBP cDNAs, the mRNA abundance generally reflected the media IGFBP content. The expression of IGFBP-4 mRNA in vivo was examined as well and compared to that of IGFBP-1 and IGFBP-2. Transcripts for both IGFBP-2 and IGFBP-4 were found in all gross anatomical divisions of the rat brain from embryonic day 15 until adulthood, whereas IGFBP-1 was not detected at any time. IGFBP-4 mRNA tended to be more abundant at the youngest ages whereas IGFBP-2 increased during development. These data indicate that IGFBP-4 is produce by a variety of neural cell types and suggest that it may play a role in brain development.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpepen_US
dc.relation.ispartofRegulatory Peptidesen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshAntibodiesen_US
dc.subject.meshAstrocytes - Metabolismen_US
dc.subject.meshAstrocytoma - Metabolismen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCarrier Proteins - Analysis - Biosynthesisen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCerebral Cortex - Metabolismen_US
dc.subject.meshFetusen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHumansen_US
dc.subject.meshInsulin-Like Growth Factor Binding Protein 1en_US
dc.subject.meshInsulin-Like Growth Factor Binding Protein 2en_US
dc.subject.meshInsulin-Like Growth Factor Binding Protein 4en_US
dc.subject.meshLiver - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeurons - Metabolismen_US
dc.subject.meshPeptides - Chemical Synthesis - Immunologyen_US
dc.subject.meshRna, Messenger - Analysis - Biosynthesisen_US
dc.subject.meshRatsen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleExpression of insulin-like growth factor binding protein-4 (IGFBP-4) by rat neural cells - Comparison to other IGFBPsen_US
dc.typeArticleen_US
dc.identifier.emailCheung, PT:ptcheung@hkucc.hku.hken_US
dc.identifier.authorityCheung, PT=rp00351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0167-0115(93)90341-5en_US
dc.identifier.pmid7505459-
dc.identifier.scopuseid_2-s2.0-0027438723en_US
dc.identifier.volume48en_US
dc.identifier.issue1-2en_US
dc.identifier.spage123en_US
dc.identifier.epage132en_US
dc.identifier.isiWOS:A1993MD63700013-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChernausek, SD=7005403226en_US
dc.identifier.scopusauthoridMurray, MA=36807464600en_US
dc.identifier.scopusauthoridCheung, PT=7202595465en_US

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