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Article: Interferon and phorbol esters down-regulate sIgM expression by independent pathways

TitleInterferon and phorbol esters down-regulate sIgM expression by independent pathways
Authors
Issue Date1988
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 1988, v. 134 n. 2, p. 245-252 How to Cite?
AbstractWe studied the effects of recombinant interferon, 12-0-tetradecanoylphorbol-13-acetate (TPA), and phorbol 12,13 dibutyrate (PDB) on surface immunoglobulin expression by Daudi cells. Incubation of cells with recombinant alpha 2 interferon (IFN-α 2) caused a 2.5-fold (60%) decrease in sIgM expression as measured by relative fluorescence index (RFI) using a flow cytometer. This decrease in sIgM expression was independent of inhibitory effects on proliferation and cell cycle progression. TPA or PDB also caused a threefold (67%) decrease in sIgM expression, while enhancing proliferation and cell cycle progression. Coincubation of cells with IFN-α 2 and TPA decreased sIgM expression by more than fourfold (> 75%), which was greater than the decrease induced by the optimal concentration of either agent alone. Molecular studies demonstrated that the treatment of cells with IFN-α 2 or TPA decreased the steady-state levels of mRNA for the heavy chain of IgM (cμ), suggesting that down-regulation of sIgM occurred at a pretranslational level. Activation of the cell membrane sodium/proton antiport did not play an integral role in the IFN-α 2 or phorbol-ester-induced pathway of sIgM down-regulation. Whereas IFN-α 2 induced an increase in the activity of 2',5'-oligoadenylate (2-5A) synthetase, the addition of TPA to IFN-α 2 caused a significant decrease in the activity of this enzyme. Although IFN-α 2 and TPA exhibited additive effects on sIgM expression, they had opposing effects on cell proliferation, cell cycle progression, and induction of 2-5A synthetase activity, suggesting that these agents down-regulate sIgM expression through independent pathways.
Persistent Identifierhttp://hdl.handle.net/10722/170230
ISSN
2015 Impact Factor: 4.155
2015 SCImago Journal Rankings: 1.842
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSchaffer, FMen_US
dc.contributor.authorBenedict, SHen_US
dc.contributor.authorPetsche, Den_US
dc.contributor.authorLau, Aen_US
dc.contributor.authorWilliams, BRGen_US
dc.contributor.authorMills, GBen_US
dc.contributor.authorGelfand, EWen_US
dc.date.accessioned2012-10-30T06:06:51Z-
dc.date.available2012-10-30T06:06:51Z-
dc.date.issued1988en_US
dc.identifier.citationJournal Of Cellular Physiology, 1988, v. 134 n. 2, p. 245-252en_US
dc.identifier.issn0021-9541en_US
dc.identifier.urihttp://hdl.handle.net/10722/170230-
dc.description.abstractWe studied the effects of recombinant interferon, 12-0-tetradecanoylphorbol-13-acetate (TPA), and phorbol 12,13 dibutyrate (PDB) on surface immunoglobulin expression by Daudi cells. Incubation of cells with recombinant alpha 2 interferon (IFN-α 2) caused a 2.5-fold (60%) decrease in sIgM expression as measured by relative fluorescence index (RFI) using a flow cytometer. This decrease in sIgM expression was independent of inhibitory effects on proliferation and cell cycle progression. TPA or PDB also caused a threefold (67%) decrease in sIgM expression, while enhancing proliferation and cell cycle progression. Coincubation of cells with IFN-α 2 and TPA decreased sIgM expression by more than fourfold (> 75%), which was greater than the decrease induced by the optimal concentration of either agent alone. Molecular studies demonstrated that the treatment of cells with IFN-α 2 or TPA decreased the steady-state levels of mRNA for the heavy chain of IgM (cμ), suggesting that down-regulation of sIgM occurred at a pretranslational level. Activation of the cell membrane sodium/proton antiport did not play an integral role in the IFN-α 2 or phorbol-ester-induced pathway of sIgM down-regulation. Whereas IFN-α 2 induced an increase in the activity of 2',5'-oligoadenylate (2-5A) synthetase, the addition of TPA to IFN-α 2 caused a significant decrease in the activity of this enzyme. Although IFN-α 2 and TPA exhibited additive effects on sIgM expression, they had opposing effects on cell proliferation, cell cycle progression, and induction of 2-5A synthetase activity, suggesting that these agents down-regulate sIgM expression through independent pathways.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_US
dc.relation.ispartofJournal of Cellular Physiologyen_US
dc.subject.mesh2',5'-Oligoadenylate Synthetase - Metabolismen_US
dc.subject.meshCarrier Proteins - Metabolismen_US
dc.subject.meshCell Cycle - Drug Effectsen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCycloheximide - Pharmacologyen_US
dc.subject.meshEnzyme Activation - Drug Effectsen_US
dc.subject.meshImmunoglobulin M - Metabolismen_US
dc.subject.meshInterferon Type I - Pharmacologyen_US
dc.subject.meshLymphocytes - Cytology - Metabolismen_US
dc.subject.meshPhorbol 12,13-Dibutyrateen_US
dc.subject.meshPhorbol Esters - Pharmacologyen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshReceptors, Antigen, B-Cell - Metabolismen_US
dc.subject.meshSodium-Hydrogen Antiporteren_US
dc.subject.meshTetradecanoylphorbol Acetate - Pharmacologyen_US
dc.subject.meshThymidine - Metabolismen_US
dc.titleInterferon and phorbol esters down-regulate sIgM expression by independent pathwaysen_US
dc.typeArticleen_US
dc.identifier.emailLau, A:asylau@hku.hken_US
dc.identifier.authorityLau, A=rp00474en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jcp.1041340210-
dc.identifier.pmid2831237en_US
dc.identifier.scopuseid_2-s2.0-0023850379en_US
dc.identifier.volume134en_US
dc.identifier.issue2en_US
dc.identifier.spage245en_US
dc.identifier.epage252en_US
dc.identifier.isiWOS:A1988M290100009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSchaffer, FM=55283556700en_US
dc.identifier.scopusauthoridBenedict, SH=7006601547en_US
dc.identifier.scopusauthoridPetsche, D=6602448825en_US
dc.identifier.scopusauthoridLau, A=7202626202en_US
dc.identifier.scopusauthoridWilliams, BRG=7404502964en_US
dc.identifier.scopusauthoridMills, GB=35379638300en_US
dc.identifier.scopusauthoridGelfand, EW=36041298200en_US

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