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Article: The marmoset B-lymphoblastoid cell line (B95-8) produces and responds to B-cell growth and differentiation factors: Role of shed CD23 (sCD23)

TitleThe marmoset B-lymphoblastoid cell line (B95-8) produces and responds to B-cell growth and differentiation factors: Role of shed CD23 (sCD23)
Authors
Issue Date1988
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMM
Citation
Immunology, 1988, v. 65 n. 3, p. 379-384 How to Cite?
AbstractThe EBV-producing marmoset B-cell line (B95-8), commonly used as a source of EBV for stimulation and transformation of human B cells, was shown to proliferate in response to supernatants containing human B-cell growth factors (BCGF) derived from PHA-activated T cells or the KG-la cell line, and to a commercial low molecular weight BCGF (BCGF(low)), but not to recombinant human IL-4 (rhIL-4). In this respect, B95-8 responded in much the same way as human EBV-transformed lymphoblastoid cell lines (LCL). In contrast, B95-8 did not secrete immunoglobulin in response to B-cell differentiation factor (BCDF) containing supernatants from the KG-la cell line, nor to BCGF(low), or IL-6 obtained from the T24 bladder carcinoma cell line, whereas significant responses were obtained with human EBV-transformed LCL. Both B95-8 and control EBV-transformed human LCL secreted BCDF detected with the indicator B-cell lines CESS, L4, and HFB1, but only the human LCL secreted BCGF detectable in co-stimulation assays with TPA-activated tonsillar B cells. Unlike EBV-transformed LCL, B95-8 did not express detectable surface CD23, and did not release into the culture medium soluble CD23 (sCD23) recognized by an EIA for the human molecule. Although not releasing detectable sCD23, B95-8 cells did proliferate in response to purified human sCD23, and were found to be 1000 times more sensitive in this assay than EBV-transformed LCL. This may provide a basis for a sensitive bioassay for sCD23. Unlike EBV-transformed LCL, it seems that in vitro proliferation of B95-8 may involve an autocrine loop which does not depend on CD23.
Persistent Identifierhttp://hdl.handle.net/10722/170229
ISSN
2015 Impact Factor: 4.078
2015 SCImago Journal Rankings: 2.038
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCallard, REen_US
dc.contributor.authorLau, YLen_US
dc.contributor.authorShields, JGen_US
dc.contributor.authorSmith, SHen_US
dc.contributor.authorCairns, Jen_US
dc.contributor.authorFloresRomo, Len_US
dc.contributor.authorGordon, Jen_US
dc.date.accessioned2012-10-30T06:06:50Z-
dc.date.available2012-10-30T06:06:50Z-
dc.date.issued1988en_US
dc.identifier.citationImmunology, 1988, v. 65 n. 3, p. 379-384en_US
dc.identifier.issn0019-2805en_US
dc.identifier.urihttp://hdl.handle.net/10722/170229-
dc.description.abstractThe EBV-producing marmoset B-cell line (B95-8), commonly used as a source of EBV for stimulation and transformation of human B cells, was shown to proliferate in response to supernatants containing human B-cell growth factors (BCGF) derived from PHA-activated T cells or the KG-la cell line, and to a commercial low molecular weight BCGF (BCGF(low)), but not to recombinant human IL-4 (rhIL-4). In this respect, B95-8 responded in much the same way as human EBV-transformed lymphoblastoid cell lines (LCL). In contrast, B95-8 did not secrete immunoglobulin in response to B-cell differentiation factor (BCDF) containing supernatants from the KG-la cell line, nor to BCGF(low), or IL-6 obtained from the T24 bladder carcinoma cell line, whereas significant responses were obtained with human EBV-transformed LCL. Both B95-8 and control EBV-transformed human LCL secreted BCDF detected with the indicator B-cell lines CESS, L4, and HFB1, but only the human LCL secreted BCGF detectable in co-stimulation assays with TPA-activated tonsillar B cells. Unlike EBV-transformed LCL, B95-8 did not express detectable surface CD23, and did not release into the culture medium soluble CD23 (sCD23) recognized by an EIA for the human molecule. Although not releasing detectable sCD23, B95-8 cells did proliferate in response to purified human sCD23, and were found to be 1000 times more sensitive in this assay than EBV-transformed LCL. This may provide a basis for a sensitive bioassay for sCD23. Unlike EBV-transformed LCL, it seems that in vitro proliferation of B95-8 may involve an autocrine loop which does not depend on CD23.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMMen_US
dc.relation.ispartofImmunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Differentiation, B-Lymphocyte - Immunologyen_US
dc.subject.meshB-Lymphocytes - Immunologyen_US
dc.subject.meshCallitrichinaeen_US
dc.subject.meshCell Lineen_US
dc.subject.meshInterleukin-4en_US
dc.subject.meshInterleukins - Immunologyen_US
dc.subject.meshLymphocyte Activationen_US
dc.titleThe marmoset B-lymphoblastoid cell line (B95-8) produces and responds to B-cell growth and differentiation factors: Role of shed CD23 (sCD23)en_US
dc.typeArticleen_US
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_US
dc.identifier.authorityLau, YL=rp00361en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3145248-
dc.identifier.scopuseid_2-s2.0-0023791836en_US
dc.identifier.volume65en_US
dc.identifier.issue3en_US
dc.identifier.spage379en_US
dc.identifier.epage384en_US
dc.identifier.isiWOS:A1988R061000008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridCallard, RE=7005116699en_US
dc.identifier.scopusauthoridLau, YL=7201403380en_US
dc.identifier.scopusauthoridShields, JG=7402027856en_US
dc.identifier.scopusauthoridSmith, SH=7406655276en_US
dc.identifier.scopusauthoridCairns, J=36840727600en_US
dc.identifier.scopusauthoridFloresRomo, L=7004304349en_US
dc.identifier.scopusauthoridGordon, J=7404625068en_US

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