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Article: Differential human interferon α receptor expression on proliferating and non-proliferating cells

TitleDifferential human interferon α receptor expression on proliferating and non-proliferating cells
Authors
Issue Date1986
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 1986, v. 157 n. 1, p. 183-193 How to Cite?
AbstractThe expression of interferon-α (IFN-α) receptors was studied on a variety of human cells, using monoiodinated IFN-α2 probes. Steady-state binding at 4°C revealed a single class of non-interacting IFN receptor on peripheral blood lymphocytes, and tonsillar B lymphocytes, which are both known to be G0/G1 resting cell populations. The binding affinity of this class of receptor was found to be on the order of 5 x 10-10 M, expressed as an apparent dissociation constant (K(d)). However, cells proliferating either in culture or in vivo were found to express a heterogeneity in IFN-α2 binding. Such binding could be objectively resolved (by a version of the LIGAND program of P. Munson) into a two-site receptor model. Hill plots of binding to proliferating cells indicated a negative cooperativity in the interaction of IFN and receptor. The high-affinity component, expressed on proliferating cells, typically exhibits a K(d) of (1-10) x 10-11 M, while the lower-affinity component indicates a K(d) of (1-10) x 10-9 M. Furthermore, the low-affinity component is apparently expressed on the order of 10-200 times the copy number, per cell, of the high-affinity site. Affinity-labeling experiments revealed that, in addition to the 140-160-kDa IFN-binding complex reported by others, both the proliferating and non-proliferating cell populations possess a novel IFN-binding component of 60 kDa.
Persistent Identifierhttp://hdl.handle.net/10722/170219
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHannigan, GEen_US
dc.contributor.authorLau, ASen_US
dc.contributor.authorWilliams, BRGen_US
dc.date.accessioned2012-10-30T06:06:46Z-
dc.date.available2012-10-30T06:06:46Z-
dc.date.issued1986en_US
dc.identifier.citationEuropean Journal Of Biochemistry, 1986, v. 157 n. 1, p. 183-193en_US
dc.identifier.issn0014-2956en_US
dc.identifier.urihttp://hdl.handle.net/10722/170219-
dc.description.abstractThe expression of interferon-α (IFN-α) receptors was studied on a variety of human cells, using monoiodinated IFN-α2 probes. Steady-state binding at 4°C revealed a single class of non-interacting IFN receptor on peripheral blood lymphocytes, and tonsillar B lymphocytes, which are both known to be G0/G1 resting cell populations. The binding affinity of this class of receptor was found to be on the order of 5 x 10-10 M, expressed as an apparent dissociation constant (K(d)). However, cells proliferating either in culture or in vivo were found to express a heterogeneity in IFN-α2 binding. Such binding could be objectively resolved (by a version of the LIGAND program of P. Munson) into a two-site receptor model. Hill plots of binding to proliferating cells indicated a negative cooperativity in the interaction of IFN and receptor. The high-affinity component, expressed on proliferating cells, typically exhibits a K(d) of (1-10) x 10-11 M, while the lower-affinity component indicates a K(d) of (1-10) x 10-9 M. Furthermore, the low-affinity component is apparently expressed on the order of 10-200 times the copy number, per cell, of the high-affinity site. Affinity-labeling experiments revealed that, in addition to the 140-160-kDa IFN-binding complex reported by others, both the proliferating and non-proliferating cell populations possess a novel IFN-binding component of 60 kDa.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_US
dc.relation.ispartofEuropean Journal of Biochemistryen_US
dc.subject.meshAffinity Labels - Metabolismen_US
dc.subject.meshB-Lymphocytes - Cytologyen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon Type I - Metabolismen_US
dc.subject.meshInterphaseen_US
dc.subject.meshKineticsen_US
dc.subject.meshLymphocytes - Metabolismen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshPalatine Tonsil - Cytologyen_US
dc.subject.meshReceptors, Immunologic - Biosynthesisen_US
dc.subject.meshReceptors, Interferonen_US
dc.subject.meshSoftwareen_US
dc.titleDifferential human interferon α receptor expression on proliferating and non-proliferating cellsen_US
dc.typeArticleen_US
dc.identifier.emailLau, AS:asylau@hku.hken_US
dc.identifier.authorityLau, AS=rp00474en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1432-1033.1986.tb09655.x-
dc.identifier.pmid2940085-
dc.identifier.scopuseid_2-s2.0-0023049746en_US
dc.identifier.volume157en_US
dc.identifier.issue1en_US
dc.identifier.spage183en_US
dc.identifier.epage193en_US
dc.identifier.isiWOS:A1986C635200021-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHannigan, GE=7004365563en_US
dc.identifier.scopusauthoridLau, AS=7202626202en_US
dc.identifier.scopusauthoridWilliams, BRG=7404502964en_US

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