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Article: Cis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164
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TitleCis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164
 
AuthorsTang, J2 3
Luo, Z5
Zhou, G1
Song, C3
Yu, F2
Xiang, J5 3
Li, G4 6
 
Issue Date2011
 
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmolbiol/
 
CitationBmc Molecular Biology, 2011, v. 12 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1471-2199-12-44
 
AbstractBackground: CD164 (also known as MGC-24v or endolyn) is a sialomucin which has been suggested to participate in regulating the proliferation, cell adhesion and differentiation of hematopoietic stem and progenitor cells. CD164 is also involved in the development of cancer. The functions of cis-regulatory elements of CD164 remain relatively unknown.Methods: In this study, we investigated the function of cis-regulatory elements within the promoter of CD164. We fused the 5'-flanking region of CD164 to a luciferase reporter vector. The minimal promoter region was confirmed by luciferase reporter assay. Using in silico analysis, we found the presence of one HIF-1alpha (HIF-1A) motif (5_-RCGTG-3_) overlapping E-box (CACGTG) and two AP-1-like binding sites (CGCTGTCCC, GTCTGTTG), one of which is also overlapped with HIF-1alpha sequence. Dual-luciferase assay was performed to examine the transcriptional activity of AP-1 and HIF-1alpha of CD164 promoter. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure CD164 expression. Chromatin Immunoprecipitation was used to confirm the binding of HIF-1alpha and CD164.Results: Co-transfection of c-jun, HIF-1alpha and minimal promoter region construct demonstrated that c-jun and HIF-1alpha bound the CD164 promoter and promoted CD164 expression. Hypoxia treatment also led to the up-regulation of CD164 expression. The mutation of overlapping sequences resulted in the reduced expression of CD164 induced by HIF-1alpha. Chromatin Immunoprecipitation demonstrated that the HIF-1alpha bound the minimal promoter region.Conclusions: Determination of the optimal promoter region and transcription factors governing CD164 expression is useful in understanding CD164 functions. These results suggest that cis-regulatory elements of CD164 overlapping HIF-1alpha/E-box/AP-1-like sequences may play important regulatory roles. © 2011 Tang et al; licensee BioMed Central Ltd.
 
ISSN1471-2199
2012 Impact Factor: 2.796
2012 SCImago Journal Rankings: 1.154
 
DOIhttp://dx.doi.org/10.1186/1471-2199-12-44
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTang, J
 
dc.contributor.authorLuo, Z
 
dc.contributor.authorZhou, G
 
dc.contributor.authorSong, C
 
dc.contributor.authorYu, F
 
dc.contributor.authorXiang, J
 
dc.contributor.authorLi, G
 
dc.date.accessioned2012-10-30T06:05:56Z
 
dc.date.available2012-10-30T06:05:56Z
 
dc.date.issued2011
 
dc.description.abstractBackground: CD164 (also known as MGC-24v or endolyn) is a sialomucin which has been suggested to participate in regulating the proliferation, cell adhesion and differentiation of hematopoietic stem and progenitor cells. CD164 is also involved in the development of cancer. The functions of cis-regulatory elements of CD164 remain relatively unknown.Methods: In this study, we investigated the function of cis-regulatory elements within the promoter of CD164. We fused the 5'-flanking region of CD164 to a luciferase reporter vector. The minimal promoter region was confirmed by luciferase reporter assay. Using in silico analysis, we found the presence of one HIF-1alpha (HIF-1A) motif (5_-RCGTG-3_) overlapping E-box (CACGTG) and two AP-1-like binding sites (CGCTGTCCC, GTCTGTTG), one of which is also overlapped with HIF-1alpha sequence. Dual-luciferase assay was performed to examine the transcriptional activity of AP-1 and HIF-1alpha of CD164 promoter. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure CD164 expression. Chromatin Immunoprecipitation was used to confirm the binding of HIF-1alpha and CD164.Results: Co-transfection of c-jun, HIF-1alpha and minimal promoter region construct demonstrated that c-jun and HIF-1alpha bound the CD164 promoter and promoted CD164 expression. Hypoxia treatment also led to the up-regulation of CD164 expression. The mutation of overlapping sequences resulted in the reduced expression of CD164 induced by HIF-1alpha. Chromatin Immunoprecipitation demonstrated that the HIF-1alpha bound the minimal promoter region.Conclusions: Determination of the optimal promoter region and transcription factors governing CD164 expression is useful in understanding CD164 functions. These results suggest that cis-regulatory elements of CD164 overlapping HIF-1alpha/E-box/AP-1-like sequences may play important regulatory roles. © 2011 Tang et al; licensee BioMed Central Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBmc Molecular Biology, 2011, v. 12 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1471-2199-12-44
 
dc.identifier.citeulike9904185
 
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2199-12-44
 
dc.identifier.issn1471-2199
2012 Impact Factor: 2.796
2012 SCImago Journal Rankings: 1.154
 
dc.identifier.scopuseid_2-s2.0-80054006537
 
dc.identifier.urihttp://hdl.handle.net/10722/170185
 
dc.identifier.volume12
 
dc.languageeng
 
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmolbiol/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofBMC Molecular Biology
 
dc.relation.referencesReferences in Scopus
 
dc.titleCis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164
 
dc.typeArticle
 
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<contributor.author>Song, C</contributor.author>
<contributor.author>Yu, F</contributor.author>
<contributor.author>Xiang, J</contributor.author>
<contributor.author>Li, G</contributor.author>
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<description.abstract>Background: CD164 (also known as MGC-24v or endolyn) is a sialomucin which has been suggested to participate in regulating the proliferation, cell adhesion and differentiation of hematopoietic stem and progenitor cells. CD164 is also involved in the development of cancer. The functions of cis-regulatory elements of CD164 remain relatively unknown.Methods: In this study, we investigated the function of cis-regulatory elements within the promoter of CD164. We fused the 5&apos;-flanking region of CD164 to a luciferase reporter vector. The minimal promoter region was confirmed by luciferase reporter assay. Using in silico analysis, we found the presence of one HIF-1alpha (HIF-1A) motif (5_-RCGTG-3_) overlapping E-box (CACGTG) and two AP-1-like binding sites (CGCTGTCCC, GTCTGTTG), one of which is also overlapped with HIF-1alpha sequence. Dual-luciferase assay was performed to examine the transcriptional activity of AP-1 and HIF-1alpha of CD164 promoter. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure CD164 expression. Chromatin Immunoprecipitation was used to confirm the binding of HIF-1alpha and CD164.Results: Co-transfection of c-jun, HIF-1alpha and minimal promoter region construct demonstrated that c-jun and HIF-1alpha bound the CD164 promoter and promoted CD164 expression. Hypoxia treatment also led to the up-regulation of CD164 expression. The mutation of overlapping sequences resulted in the reduced expression of CD164 induced by HIF-1alpha. Chromatin Immunoprecipitation demonstrated that the HIF-1alpha bound the minimal promoter region.Conclusions: Determination of the optimal promoter region and transcription factors governing CD164 expression is useful in understanding CD164 functions. These results suggest that cis-regulatory elements of CD164 overlapping HIF-1alpha/E-box/AP-1-like sequences may play important regulatory roles. &#169; 2011 Tang et al; licensee BioMed Central Ltd.</description.abstract>
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Author Affiliations
  1. Shenzhen University
  2. Second Xiangya Hospital of Central-South University
  3. Queen's University Belfast
  4. Prince of Wales Hospital Hong Kong
  5. Central South University China
  6. Chinese University of Hong Kong