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Article: Cis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164

TitleCis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164
Authors
Issue Date2011
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmolbiol/
Citation
Bmc Molecular Biology, 2011, v. 12 How to Cite?
Abstract
Background: CD164 (also known as MGC-24v or endolyn) is a sialomucin which has been suggested to participate in regulating the proliferation, cell adhesion and differentiation of hematopoietic stem and progenitor cells. CD164 is also involved in the development of cancer. The functions of cis-regulatory elements of CD164 remain relatively unknown.Methods: In this study, we investigated the function of cis-regulatory elements within the promoter of CD164. We fused the 5'-flanking region of CD164 to a luciferase reporter vector. The minimal promoter region was confirmed by luciferase reporter assay. Using in silico analysis, we found the presence of one HIF-1alpha (HIF-1A) motif (5_-RCGTG-3_) overlapping E-box (CACGTG) and two AP-1-like binding sites (CGCTGTCCC, GTCTGTTG), one of which is also overlapped with HIF-1alpha sequence. Dual-luciferase assay was performed to examine the transcriptional activity of AP-1 and HIF-1alpha of CD164 promoter. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure CD164 expression. Chromatin Immunoprecipitation was used to confirm the binding of HIF-1alpha and CD164.Results: Co-transfection of c-jun, HIF-1alpha and minimal promoter region construct demonstrated that c-jun and HIF-1alpha bound the CD164 promoter and promoted CD164 expression. Hypoxia treatment also led to the up-regulation of CD164 expression. The mutation of overlapping sequences resulted in the reduced expression of CD164 induced by HIF-1alpha. Chromatin Immunoprecipitation demonstrated that the HIF-1alpha bound the minimal promoter region.Conclusions: Determination of the optimal promoter region and transcription factors governing CD164 expression is useful in understanding CD164 functions. These results suggest that cis-regulatory elements of CD164 overlapping HIF-1alpha/E-box/AP-1-like sequences may play important regulatory roles. © 2011 Tang et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/170185
ISSN
2013 Impact Factor: 2.057
2013 SCImago Journal Rankings: 1.614
ISI Accession Number ID
References

 

Author Affiliations
  1. Shenzhen University
  2. Second Xiangya Hospital of Central-South University
  3. Queen's University Belfast
  4. Prince of Wales Hospital Hong Kong
  5. Central South University China
  6. Chinese University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorTang, Jen_US
dc.contributor.authorLuo, Zen_US
dc.contributor.authorZhou, Gen_US
dc.contributor.authorSong, Cen_US
dc.contributor.authorYu, Fen_US
dc.contributor.authorXiang, Jen_US
dc.contributor.authorLi, Gen_US
dc.date.accessioned2012-10-30T06:05:56Z-
dc.date.available2012-10-30T06:05:56Z-
dc.date.issued2011en_US
dc.identifier.citationBmc Molecular Biology, 2011, v. 12en_US
dc.identifier.issn1471-2199en_US
dc.identifier.urihttp://hdl.handle.net/10722/170185-
dc.description.abstractBackground: CD164 (also known as MGC-24v or endolyn) is a sialomucin which has been suggested to participate in regulating the proliferation, cell adhesion and differentiation of hematopoietic stem and progenitor cells. CD164 is also involved in the development of cancer. The functions of cis-regulatory elements of CD164 remain relatively unknown.Methods: In this study, we investigated the function of cis-regulatory elements within the promoter of CD164. We fused the 5'-flanking region of CD164 to a luciferase reporter vector. The minimal promoter region was confirmed by luciferase reporter assay. Using in silico analysis, we found the presence of one HIF-1alpha (HIF-1A) motif (5_-RCGTG-3_) overlapping E-box (CACGTG) and two AP-1-like binding sites (CGCTGTCCC, GTCTGTTG), one of which is also overlapped with HIF-1alpha sequence. Dual-luciferase assay was performed to examine the transcriptional activity of AP-1 and HIF-1alpha of CD164 promoter. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure CD164 expression. Chromatin Immunoprecipitation was used to confirm the binding of HIF-1alpha and CD164.Results: Co-transfection of c-jun, HIF-1alpha and minimal promoter region construct demonstrated that c-jun and HIF-1alpha bound the CD164 promoter and promoted CD164 expression. Hypoxia treatment also led to the up-regulation of CD164 expression. The mutation of overlapping sequences resulted in the reduced expression of CD164 induced by HIF-1alpha. Chromatin Immunoprecipitation demonstrated that the HIF-1alpha bound the minimal promoter region.Conclusions: Determination of the optimal promoter region and transcription factors governing CD164 expression is useful in understanding CD164 functions. These results suggest that cis-regulatory elements of CD164 overlapping HIF-1alpha/E-box/AP-1-like sequences may play important regulatory roles. © 2011 Tang et al; licensee BioMed Central Ltd.en_US
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmolbiol/en_US
dc.relation.ispartofBMC Molecular Biologyen_US
dc.titleCis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164en_US
dc.typeArticleen_US
dc.identifier.emailZhou, G:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, G=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1186/1471-2199-12-44en_US
dc.identifier.pmid21999799-
dc.identifier.scopuseid_2-s2.0-80054006537en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80054006537&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume12en_US
dc.identifier.isiWOS:000296970900001-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTang, J=7404639137en_US
dc.identifier.scopusauthoridLuo, Z=51864114300en_US
dc.identifier.scopusauthoridZhou, G=23394245100en_US
dc.identifier.scopusauthoridSong, C=7403252991en_US
dc.identifier.scopusauthoridYu, F=8424228100en_US
dc.identifier.scopusauthoridXiang, J=7201546177en_US
dc.identifier.scopusauthoridLi, G=36013406300en_US
dc.identifier.citeulike9904185-

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