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Article: The effect of strontium incorporation in hydroxyapatite on osteoblasts in vitro

TitleThe effect of strontium incorporation in hydroxyapatite on osteoblasts in vitro
Authors
Issue Date2011
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530
Citation
Journal Of Materials Science: Materials In Medicine, 2011, v. 22 n. 4, p. 961-967 How to Cite?
AbstractThe purpose of this study was to test the effects of a series of strontium-substituted HA (Sr-HA) ceramics (0, 1, 5, and 10 mol% Sr substitution) on osteoblasts, thereby demonstrating whether strontium incorporation with HA would favor osteoblast metabolism. Rat primary osteoblasts were cultured with culture media containing ions released from the Sr-HA ceramics as they dissolved. MTT test, alkaline phosphatase activity, osteoblast transcription factor gene (cbfa1) expression and Alizarin Red staining were conducted at different time-points. There is no significant difference in cell proliferation between groups. However, compared with HA group, Sr-HA groups presented significant enhancement with regard to ALP activity, cbfa1 mRNA expression, and mineralization nodules. Among Sr-HA groups, 5 and 10% groups showed much better performances in ALP activity, cbfa1 mRNA expression, and mineralization nodules than 1% group, however, no significant difference was found between 5 and 10% groups. This study has demonstrated that Sr incorporation in HA ceramic enhanced osteoblastic cell differentiation and mineralization. However, further detailed studies are needed to understand the mechanistic effects of this Sr incorporation on osteoblastic cells and the optimal percentage of calcium should be substituted with strontium in HA. © Springer Science+Business Media, LLC 2011.
Persistent Identifierhttp://hdl.handle.net/10722/170179
ISSN
2015 Impact Factor: 2.272
2013 SCImago Journal Rankings: 0.897
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNi, GXen_US
dc.contributor.authorYao, ZPen_US
dc.contributor.authorHuang, GTen_US
dc.contributor.authorLiu, WGen_US
dc.contributor.authorLu, WWen_US
dc.date.accessioned2012-10-30T06:05:52Z-
dc.date.available2012-10-30T06:05:52Z-
dc.date.issued2011en_US
dc.identifier.citationJournal Of Materials Science: Materials In Medicine, 2011, v. 22 n. 4, p. 961-967en_US
dc.identifier.issn0957-4530en_US
dc.identifier.urihttp://hdl.handle.net/10722/170179-
dc.description.abstractThe purpose of this study was to test the effects of a series of strontium-substituted HA (Sr-HA) ceramics (0, 1, 5, and 10 mol% Sr substitution) on osteoblasts, thereby demonstrating whether strontium incorporation with HA would favor osteoblast metabolism. Rat primary osteoblasts were cultured with culture media containing ions released from the Sr-HA ceramics as they dissolved. MTT test, alkaline phosphatase activity, osteoblast transcription factor gene (cbfa1) expression and Alizarin Red staining were conducted at different time-points. There is no significant difference in cell proliferation between groups. However, compared with HA group, Sr-HA groups presented significant enhancement with regard to ALP activity, cbfa1 mRNA expression, and mineralization nodules. Among Sr-HA groups, 5 and 10% groups showed much better performances in ALP activity, cbfa1 mRNA expression, and mineralization nodules than 1% group, however, no significant difference was found between 5 and 10% groups. This study has demonstrated that Sr incorporation in HA ceramic enhanced osteoblastic cell differentiation and mineralization. However, further detailed studies are needed to understand the mechanistic effects of this Sr incorporation on osteoblastic cells and the optimal percentage of calcium should be substituted with strontium in HA. © Springer Science+Business Media, LLC 2011.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530en_US
dc.relation.ispartofJournal of Materials Science: Materials in Medicineen_US
dc.subject.meshAlkaline Phosphatase - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnthraquinones - Pharmacologyen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCore Binding Factor Alpha 1 Subunit - Biosynthesisen_US
dc.subject.meshDurapatite - Chemistryen_US
dc.subject.meshIonsen_US
dc.subject.meshMaterials Testingen_US
dc.subject.meshOsteoblasts - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshStrontium - Chemistryen_US
dc.subject.meshTensile Strengthen_US
dc.subject.meshTime Factorsen_US
dc.titleThe effect of strontium incorporation in hydroxyapatite on osteoblasts in vitroen_US
dc.typeArticleen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s10856-011-4264-0en_US
dc.identifier.pmid21365296-
dc.identifier.scopuseid_2-s2.0-80051672618en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80051672618&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume22en_US
dc.identifier.issue4en_US
dc.identifier.spage961en_US
dc.identifier.epage967en_US
dc.identifier.isiWOS:000290044000022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNi, GX=8303037400en_US
dc.identifier.scopusauthoridYao, ZP=25724009700en_US
dc.identifier.scopusauthoridHuang, GT=26025946000en_US
dc.identifier.scopusauthoridLiu, WG=53881586300en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US
dc.identifier.citeulike8962985-

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