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Article: Rapid repair of rat sciatic nerve injury using a nanosilver-embedded collagen scaffold coated with laminin and fibronectin

TitleRapid repair of rat sciatic nerve injury using a nanosilver-embedded collagen scaffold coated with laminin and fibronectin
Authors
Issue Date2011
PublisherFuture Medicine Ltd. The Journal's web site is located at http://www.futuremedicine.com/page/loi/rme
Citation
Regenerative Medicine, 2011, v. 6 n. 4, p. 437-447 How to Cite?
AbstractAim: Scaffold with micro-channels has shown great promise in facilitating axonal regeneration after peripheral nerve injury. Significant research has focused on mimicking, in terms of composition and function, the ability of the basement membrane of Schwann cells to both promote and guide axonal regeneration. We aim to investigate the ability of a tissue-engineered scaffold with nanosilver and collagen to adsorb laminin and fibronectin, and the usefulness of this scaffold for repairing and regenerating a 10-mm peripheral nerve gap in rats. Methods: In this study, nanosilver-embedded collagen scaffolds were prepared and coated with laminin (LN) or LN plus fibronectin (FN). Scanning electron microscopy of the transverse and longitudinal sections of the scaffold revealed axially oriented microtubules ranging from 20 to 80 μm in diameter, and the internal surface of microtubules was found to be evenly coated with LN and FN. Energy dispersive spectrometry also confirmed an even distribution of nanosilver particles within the scaffold. To test its effectiveness in restoring neuronal connection, the scaffold was used in order to bridge 10 mm gaps in the severed sciatic nerve of rats. The rats were divided into an experimental group (receiving scaffold coated with LN and FN), a control group (receiving scaffold coated with LN only) and an autologous graft group. The functional recovery 40 days after surgery was examined by electrophysiology and sciatic nerve functional index (SFI) evaluation. FluoroGold™ (FG) retrograde tracing, toluidine blue staining and transmission electron microscopy were also used to examine the regenerated nerve fibers and to establish their myelination status. Results: The experimental group displayed partially restored nerve function. The recovery was comparable to the effect of autologous nerve graft and was better than that observed in the control group. A better functional recovery correlated with more FG-labeled neurons, higher density of toluidine blue stained nerve fibers and thicker myelin sheath. Conclusion: Our results demonstrated that nanosilver-embedded collagen scaffolds with LN and FN coating is effective in aiding axonal regeneration, and recovery is comparable to the effect of an autologous nerve graft. © 2011 Future Medicine Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/170177
ISSN
2014 Impact Factor: 2.786
2014 SCImago Journal Rankings: 0.754
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDing, Ten_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorZheng, Yen_US
dc.contributor.authorLi, ZYen_US
dc.contributor.authorPan, HBen_US
dc.contributor.authorLuo, Zen_US
dc.date.accessioned2012-10-30T06:05:51Z-
dc.date.available2012-10-30T06:05:51Z-
dc.date.issued2011en_US
dc.identifier.citationRegenerative Medicine, 2011, v. 6 n. 4, p. 437-447en_US
dc.identifier.issn1746-0751en_US
dc.identifier.urihttp://hdl.handle.net/10722/170177-
dc.description.abstractAim: Scaffold with micro-channels has shown great promise in facilitating axonal regeneration after peripheral nerve injury. Significant research has focused on mimicking, in terms of composition and function, the ability of the basement membrane of Schwann cells to both promote and guide axonal regeneration. We aim to investigate the ability of a tissue-engineered scaffold with nanosilver and collagen to adsorb laminin and fibronectin, and the usefulness of this scaffold for repairing and regenerating a 10-mm peripheral nerve gap in rats. Methods: In this study, nanosilver-embedded collagen scaffolds were prepared and coated with laminin (LN) or LN plus fibronectin (FN). Scanning electron microscopy of the transverse and longitudinal sections of the scaffold revealed axially oriented microtubules ranging from 20 to 80 μm in diameter, and the internal surface of microtubules was found to be evenly coated with LN and FN. Energy dispersive spectrometry also confirmed an even distribution of nanosilver particles within the scaffold. To test its effectiveness in restoring neuronal connection, the scaffold was used in order to bridge 10 mm gaps in the severed sciatic nerve of rats. The rats were divided into an experimental group (receiving scaffold coated with LN and FN), a control group (receiving scaffold coated with LN only) and an autologous graft group. The functional recovery 40 days after surgery was examined by electrophysiology and sciatic nerve functional index (SFI) evaluation. FluoroGold™ (FG) retrograde tracing, toluidine blue staining and transmission electron microscopy were also used to examine the regenerated nerve fibers and to establish their myelination status. Results: The experimental group displayed partially restored nerve function. The recovery was comparable to the effect of autologous nerve graft and was better than that observed in the control group. A better functional recovery correlated with more FG-labeled neurons, higher density of toluidine blue stained nerve fibers and thicker myelin sheath. Conclusion: Our results demonstrated that nanosilver-embedded collagen scaffolds with LN and FN coating is effective in aiding axonal regeneration, and recovery is comparable to the effect of an autologous nerve graft. © 2011 Future Medicine Ltd.en_US
dc.languageengen_US
dc.publisherFuture Medicine Ltd. The Journal's web site is located at http://www.futuremedicine.com/page/loi/rmeen_US
dc.relation.ispartofRegenerative Medicineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCoated Materials, Biocompatible - Pharmacologyen_US
dc.subject.meshCollagen - Pharmacologyen_US
dc.subject.meshFibronectins - Pharmacologyen_US
dc.subject.meshLaminin - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshNanoparticles - Chemistryen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSciatic Nerve - Injuries - Pathology - Physiopathology - Ultrastructureen_US
dc.subject.meshSilver - Pharmacologyen_US
dc.subject.meshStaining And Labelingen_US
dc.subject.meshTissue Scaffolds - Chemistryen_US
dc.subject.meshTolonium Chloride - Metabolismen_US
dc.subject.meshWound Healing - Drug Effectsen_US
dc.titleRapid repair of rat sciatic nerve injury using a nanosilver-embedded collagen scaffold coated with laminin and fibronectinen_US
dc.typeArticleen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.emailPan, HB:haobo@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.identifier.authorityPan, HB=rp01564en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2217/rme.11.39en_US
dc.identifier.pmid21749202en_US
dc.identifier.scopuseid_2-s2.0-79960239996en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960239996&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6en_US
dc.identifier.issue4en_US
dc.identifier.spage437en_US
dc.identifier.epage447en_US
dc.identifier.isiWOS:000293260700007-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridDing, T=15821940500en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US
dc.identifier.scopusauthoridZheng, Y=54586346000en_US
dc.identifier.scopusauthoridLi, ZY=35727919500en_US
dc.identifier.scopusauthoridPan, HB=7403295092en_US
dc.identifier.scopusauthoridLuo, Z=8510080000en_US
dc.identifier.citeulike9560874-

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