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Article: Rapid repair of rat sciatic nerve injury using a nanosilver-embedded collagen scaffold coated with laminin and fibronectin
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TitleRapid repair of rat sciatic nerve injury using a nanosilver-embedded collagen scaffold coated with laminin and fibronectin
 
AuthorsDing, T1
Lu, WW2
Zheng, Y1
Li, ZY2
Pan, HB2
Luo, Z1
 
Issue Date2011
 
PublisherFuture Medicine Ltd. The Journal's web site is located at http://www.futuremedicine.com/page/loi/rme
 
CitationRegenerative Medicine, 2011, v. 6 n. 4, p. 437-447 [How to Cite?]
DOI: http://dx.doi.org/10.2217/rme.11.39
 
AbstractAim: Scaffold with micro-channels has shown great promise in facilitating axonal regeneration after peripheral nerve injury. Significant research has focused on mimicking, in terms of composition and function, the ability of the basement membrane of Schwann cells to both promote and guide axonal regeneration. We aim to investigate the ability of a tissue-engineered scaffold with nanosilver and collagen to adsorb laminin and fibronectin, and the usefulness of this scaffold for repairing and regenerating a 10-mm peripheral nerve gap in rats. Methods: In this study, nanosilver-embedded collagen scaffolds were prepared and coated with laminin (LN) or LN plus fibronectin (FN). Scanning electron microscopy of the transverse and longitudinal sections of the scaffold revealed axially oriented microtubules ranging from 20 to 80 μm in diameter, and the internal surface of microtubules was found to be evenly coated with LN and FN. Energy dispersive spectrometry also confirmed an even distribution of nanosilver particles within the scaffold. To test its effectiveness in restoring neuronal connection, the scaffold was used in order to bridge 10 mm gaps in the severed sciatic nerve of rats. The rats were divided into an experimental group (receiving scaffold coated with LN and FN), a control group (receiving scaffold coated with LN only) and an autologous graft group. The functional recovery 40 days after surgery was examined by electrophysiology and sciatic nerve functional index (SFI) evaluation. FluoroGold™ (FG) retrograde tracing, toluidine blue staining and transmission electron microscopy were also used to examine the regenerated nerve fibers and to establish their myelination status. Results: The experimental group displayed partially restored nerve function. The recovery was comparable to the effect of autologous nerve graft and was better than that observed in the control group. A better functional recovery correlated with more FG-labeled neurons, higher density of toluidine blue stained nerve fibers and thicker myelin sheath. Conclusion: Our results demonstrated that nanosilver-embedded collagen scaffolds with LN and FN coating is effective in aiding axonal regeneration, and recovery is comparable to the effect of an autologous nerve graft. © 2011 Future Medicine Ltd.
 
ISSN1746-0751
2013 Impact Factor: 3.500
 
DOIhttp://dx.doi.org/10.2217/rme.11.39
 
ISI Accession Number IDWOS:000293260700007
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorDing, T
 
dc.contributor.authorLu, WW
 
dc.contributor.authorZheng, Y
 
dc.contributor.authorLi, ZY
 
dc.contributor.authorPan, HB
 
dc.contributor.authorLuo, Z
 
dc.date.accessioned2012-10-30T06:05:51Z
 
dc.date.available2012-10-30T06:05:51Z
 
dc.date.issued2011
 
dc.description.abstractAim: Scaffold with micro-channels has shown great promise in facilitating axonal regeneration after peripheral nerve injury. Significant research has focused on mimicking, in terms of composition and function, the ability of the basement membrane of Schwann cells to both promote and guide axonal regeneration. We aim to investigate the ability of a tissue-engineered scaffold with nanosilver and collagen to adsorb laminin and fibronectin, and the usefulness of this scaffold for repairing and regenerating a 10-mm peripheral nerve gap in rats. Methods: In this study, nanosilver-embedded collagen scaffolds were prepared and coated with laminin (LN) or LN plus fibronectin (FN). Scanning electron microscopy of the transverse and longitudinal sections of the scaffold revealed axially oriented microtubules ranging from 20 to 80 μm in diameter, and the internal surface of microtubules was found to be evenly coated with LN and FN. Energy dispersive spectrometry also confirmed an even distribution of nanosilver particles within the scaffold. To test its effectiveness in restoring neuronal connection, the scaffold was used in order to bridge 10 mm gaps in the severed sciatic nerve of rats. The rats were divided into an experimental group (receiving scaffold coated with LN and FN), a control group (receiving scaffold coated with LN only) and an autologous graft group. The functional recovery 40 days after surgery was examined by electrophysiology and sciatic nerve functional index (SFI) evaluation. FluoroGold™ (FG) retrograde tracing, toluidine blue staining and transmission electron microscopy were also used to examine the regenerated nerve fibers and to establish their myelination status. Results: The experimental group displayed partially restored nerve function. The recovery was comparable to the effect of autologous nerve graft and was better than that observed in the control group. A better functional recovery correlated with more FG-labeled neurons, higher density of toluidine blue stained nerve fibers and thicker myelin sheath. Conclusion: Our results demonstrated that nanosilver-embedded collagen scaffolds with LN and FN coating is effective in aiding axonal regeneration, and recovery is comparable to the effect of an autologous nerve graft. © 2011 Future Medicine Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationRegenerative Medicine, 2011, v. 6 n. 4, p. 437-447 [How to Cite?]
DOI: http://dx.doi.org/10.2217/rme.11.39
 
dc.identifier.citeulike9560874
 
dc.identifier.doihttp://dx.doi.org/10.2217/rme.11.39
 
dc.identifier.epage447
 
dc.identifier.isiWOS:000293260700007
 
dc.identifier.issn1746-0751
2013 Impact Factor: 3.500
 
dc.identifier.issue4
 
dc.identifier.pmid21749202
 
dc.identifier.scopuseid_2-s2.0-79960239996
 
dc.identifier.spage437
 
dc.identifier.urihttp://hdl.handle.net/10722/170177
 
dc.identifier.volume6
 
dc.languageeng
 
dc.publisherFuture Medicine Ltd. The Journal's web site is located at http://www.futuremedicine.com/page/loi/rme
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofRegenerative Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshCoated Materials, Biocompatible - Pharmacology
 
dc.subject.meshCollagen - Pharmacology
 
dc.subject.meshFibronectins - Pharmacology
 
dc.subject.meshLaminin - Pharmacology
 
dc.subject.meshMale
 
dc.subject.meshNanoparticles - Chemistry
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshSciatic Nerve - Injuries - Pathology - Physiopathology - Ultrastructure
 
dc.subject.meshSilver - Pharmacology
 
dc.subject.meshStaining And Labeling
 
dc.subject.meshTissue Scaffolds - Chemistry
 
dc.subject.meshTolonium Chloride - Metabolism
 
dc.subject.meshWound Healing - Drug Effects
 
dc.titleRapid repair of rat sciatic nerve injury using a nanosilver-embedded collagen scaffold coated with laminin and fibronectin
 
dc.typeArticle
 
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<contributor.author>Pan, HB</contributor.author>
<contributor.author>Luo, Z</contributor.author>
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Author Affiliations
  1. Xijing Hospital
  2. The University of Hong Kong