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Article: Evaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal models of hemorrhagic spinal cord injury

TitleEvaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal models of hemorrhagic spinal cord injury
Authors
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2011, v. 1391, p. 24-35 How to Cite?
AbstractCell tracking using iron oxide nanoparticles has been well established in MRI. However, in experimental rat models, the intrinsic iron signal derived from erythrocytes masks the labeled cells. The research evaluated a clinically applied Gd-DTPA for T1-weighted positive enhancement for cell tracking in spinal cord injury (SCI) rat models. MSCs were labeled with jetPEI/Gd-DTPA particles to evaluate the transfection efficiency by MRI in vitro. Differentiation assays were carried out to evaluate the differentiation ability of Gd-DTPA-labeled MSCs. The Gd-DTPA-labeled MSCs were transplanted to rat SCI model and monitored by MRI in vivo. Fluorescence images were taken to confirm the MRI results. Behavior test was assessed with Basso, Beattie, and Bresnahan (BBB) scoring in 6 weeks after cell transplantation. The Gd-labeled MSCs showed a significant increase in signal intensity in T1-weighted images. After local transplantation, Gd-DTPA-labeled MSCs could be detected in SCI rat models by the persistent T1-weighted positive enhancement from 3 to 14 days. Under electronic microscope, Gd-DTPA/jetPEI complexes were mostly observed in cytoplasm. Fluorescence microscopy examination showed that the Gd-labeled MSCs survived and distributed within the injured spinal cord until 2 weeks. The Gd-labeled MSCs were identified and tracked with MRI by cross and sagittal sections. The BBB scores of the rats with labeled MSCs transplantation were significantly higher than those of control rats. Our results demonstrated that Gd-DTPA is appropriate for cell tracking in rat model of SCI, indicating that an efficient and nontoxic label method with Gd-DTPA could properly track MSCs in hemorrhage animal models. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/170174
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Yen_US
dc.contributor.authorHe, ZJen_US
dc.contributor.authorXu, Ben_US
dc.contributor.authorWu, QZen_US
dc.contributor.authorLiu, Gen_US
dc.contributor.authorZhu, Hen_US
dc.contributor.authorZhong, Qen_US
dc.contributor.authorDeng, DYen_US
dc.contributor.authorAi, Hen_US
dc.contributor.authorYue, Qen_US
dc.contributor.authorWei, Yen_US
dc.contributor.authorJun, Sen_US
dc.contributor.authorZhou, Gen_US
dc.contributor.authorGong, QYen_US
dc.date.accessioned2012-10-30T06:05:50Z-
dc.date.available2012-10-30T06:05:50Z-
dc.date.issued2011en_US
dc.identifier.citationBrain Research, 2011, v. 1391, p. 24-35en_US
dc.identifier.issn0006-8993en_US
dc.identifier.urihttp://hdl.handle.net/10722/170174-
dc.description.abstractCell tracking using iron oxide nanoparticles has been well established in MRI. However, in experimental rat models, the intrinsic iron signal derived from erythrocytes masks the labeled cells. The research evaluated a clinically applied Gd-DTPA for T1-weighted positive enhancement for cell tracking in spinal cord injury (SCI) rat models. MSCs were labeled with jetPEI/Gd-DTPA particles to evaluate the transfection efficiency by MRI in vitro. Differentiation assays were carried out to evaluate the differentiation ability of Gd-DTPA-labeled MSCs. The Gd-DTPA-labeled MSCs were transplanted to rat SCI model and monitored by MRI in vivo. Fluorescence images were taken to confirm the MRI results. Behavior test was assessed with Basso, Beattie, and Bresnahan (BBB) scoring in 6 weeks after cell transplantation. The Gd-labeled MSCs showed a significant increase in signal intensity in T1-weighted images. After local transplantation, Gd-DTPA-labeled MSCs could be detected in SCI rat models by the persistent T1-weighted positive enhancement from 3 to 14 days. Under electronic microscope, Gd-DTPA/jetPEI complexes were mostly observed in cytoplasm. Fluorescence microscopy examination showed that the Gd-labeled MSCs survived and distributed within the injured spinal cord until 2 weeks. The Gd-labeled MSCs were identified and tracked with MRI by cross and sagittal sections. The BBB scores of the rats with labeled MSCs transplantation were significantly higher than those of control rats. Our results demonstrated that Gd-DTPA is appropriate for cell tracking in rat model of SCI, indicating that an efficient and nontoxic label method with Gd-DTPA could properly track MSCs in hemorrhage animal models. © 2011 Elsevier B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_US
dc.relation.ispartofBrain Researchen_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Movement - Drug Effects - Physiologyen_US
dc.subject.meshCell Tracking - Methodsen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshFemaleen_US
dc.subject.meshGadolinium Dtpa - Diagnostic Useen_US
dc.subject.meshGreen Fluorescent Proteins - Geneticsen_US
dc.subject.meshHemorrhage - Complications - Pathologyen_US
dc.subject.meshMagnetic Resonance Imagingen_US
dc.subject.meshMaleen_US
dc.subject.meshMesenchymal Stem Cell Transplantation - Methodsen_US
dc.subject.meshMesenchymal Stem Cells - Physiologyen_US
dc.subject.meshMicroscopy, Electron, Transmissionen_US
dc.subject.meshPolymers - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshSpectrophotometryen_US
dc.subject.meshSpinal Cord Injuries - Complications - Pathology - Surgeryen_US
dc.subject.meshTransfection - Methodsen_US
dc.titleEvaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal models of hemorrhagic spinal cord injuryen_US
dc.typeArticleen_US
dc.identifier.emailZhou, G:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, G=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.brainres.2011.03.032en_US
dc.identifier.pmid21420939-
dc.identifier.scopuseid_2-s2.0-79955611165en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955611165&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1391en_US
dc.identifier.spage24en_US
dc.identifier.epage35en_US
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLiu, Y=36012292400en_US
dc.identifier.scopusauthoridHe, ZJ=8402134000en_US
dc.identifier.scopusauthoridXu, B=37102849400en_US
dc.identifier.scopusauthoridWu, QZ=21733892600en_US
dc.identifier.scopusauthoridLiu, G=36077582900en_US
dc.identifier.scopusauthoridZhu, H=37103337300en_US
dc.identifier.scopusauthoridZhong, Q=37103341500en_US
dc.identifier.scopusauthoridDeng, DY=37101300300en_US
dc.identifier.scopusauthoridAi, H=7005353373en_US
dc.identifier.scopusauthoridYue, Q=36624752200en_US
dc.identifier.scopusauthoridWei, Y=37103006600en_US
dc.identifier.scopusauthoridJun, S=37101537000en_US
dc.identifier.scopusauthoridZhou, G=23394245100en_US
dc.identifier.scopusauthoridGong, QY=7201440871en_US

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