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Article: Expression of c-Met in muscle satellite cells of muscle injury

TitleExpression of c-Met in muscle satellite cells of muscle injury
Authors
KeywordsC-Met
Cardiotoxin
Immunohistochemistry
Mouse
Muscle Injury
Muscle Satellite Cell
Issue Date2009
Citation
Acta Anatomica Sinica, 2009, v. 40 n. 5, p. 780-784 How to Cite?
AbstractObjective: To observe the expression of c-Met in muscle satellite cells in situ of normal and cardiotoxin-intoxicated muscle tissues. Methods: Twelve C 57 mice were divided into six groups, and raised in IVC laboratory. In the 1st to 6th group, the mice were injectied with cardiotoxin (5μg per mouse) in left quadriceps femoris, while their right quadriceps femoris were left for control without any injection. After the mice were sacrificed at different limited time-point. (one day, four days, one week, two weeks, four weeks, six weeks), the quadriceps femoris were isolated and fixed in 4 percent formaldehyde for 24 hours and then embeded in paraffin for histological, immunohistochemistical and reverse transcription-polymerase chain reaction (RT-PCR) analysis to detect the expression of c-Met in muscle tissues in situ. Results: The results of immunohistochemistry and RT-PCR showed that there were few expressions of c-Met in muscle satellite cells of normal muscle tissues. Compared with normal tissues, obvious expression was found in 24 hour-group as well as the sixth week-group. The expression of c-Met peeked in the 1st and expressed continuously untill the 6th week. Conclusion: c-Met may be the critical protein in the process of muscle impairment and repaining.
Persistent Identifierhttp://hdl.handle.net/10722/170151
ISSN
2015 SCImago Journal Rankings: 0.121
References

 

DC FieldValueLanguage
dc.contributor.authorLi, XLen_US
dc.contributor.authorHuang, HJen_US
dc.contributor.authorYang, ZMen_US
dc.contributor.authorZhou, GQen_US
dc.contributor.authorXie, HQen_US
dc.contributor.authorDeng, Len_US
dc.date.accessioned2012-10-30T06:05:38Z-
dc.date.available2012-10-30T06:05:38Z-
dc.date.issued2009en_US
dc.identifier.citationActa Anatomica Sinica, 2009, v. 40 n. 5, p. 780-784en_US
dc.identifier.issn0529-1356en_US
dc.identifier.urihttp://hdl.handle.net/10722/170151-
dc.description.abstractObjective: To observe the expression of c-Met in muscle satellite cells in situ of normal and cardiotoxin-intoxicated muscle tissues. Methods: Twelve C 57 mice were divided into six groups, and raised in IVC laboratory. In the 1st to 6th group, the mice were injectied with cardiotoxin (5μg per mouse) in left quadriceps femoris, while their right quadriceps femoris were left for control without any injection. After the mice were sacrificed at different limited time-point. (one day, four days, one week, two weeks, four weeks, six weeks), the quadriceps femoris were isolated and fixed in 4 percent formaldehyde for 24 hours and then embeded in paraffin for histological, immunohistochemistical and reverse transcription-polymerase chain reaction (RT-PCR) analysis to detect the expression of c-Met in muscle tissues in situ. Results: The results of immunohistochemistry and RT-PCR showed that there were few expressions of c-Met in muscle satellite cells of normal muscle tissues. Compared with normal tissues, obvious expression was found in 24 hour-group as well as the sixth week-group. The expression of c-Met peeked in the 1st and expressed continuously untill the 6th week. Conclusion: c-Met may be the critical protein in the process of muscle impairment and repaining.en_US
dc.languageengen_US
dc.relation.ispartofActa Anatomica Sinicaen_US
dc.subjectC-Meten_US
dc.subjectCardiotoxinen_US
dc.subjectImmunohistochemistryen_US
dc.subjectMouseen_US
dc.subjectMuscle Injuryen_US
dc.subjectMuscle Satellite Cellen_US
dc.titleExpression of c-Met in muscle satellite cells of muscle injuryen_US
dc.typeArticleen_US
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, GQ=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3969/j.issn.0529-1356.2009.05.017en_US
dc.identifier.scopuseid_2-s2.0-72449212230en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-72449212230&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue5en_US
dc.identifier.spage780en_US
dc.identifier.epage784en_US
dc.identifier.scopusauthoridLi, XL=35210528900en_US
dc.identifier.scopusauthoridHuang, HJ=7405610061en_US
dc.identifier.scopusauthoridYang, ZM=7405433260en_US
dc.identifier.scopusauthoridZhou, GQ=23394245100en_US
dc.identifier.scopusauthoridXie, HQ=7401672194en_US
dc.identifier.scopusauthoridDeng, L=37001461300en_US

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