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Article: Expression of CXC chemokine receptor 4 in muscle satellite cells of muscle injury tissues

TitleExpression of CXC chemokine receptor 4 in muscle satellite cells of muscle injury tissues
Authors
Issue Date2009
Citation
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal Of Reparative And Reconstructive Surgery, 2009, v. 23 n. 1, p. 26-29 How to Cite?
AbstractOBJECTIVE: To observe the expressions of CXC chemokine receptor 4 (CXCR4) in muscle satellite cells in situ of normal and cardiotoxin-intoxicated muscle tissues so as to further investigate the molecular mechanism involving in muscle regeneration such as progressing muscular dystrophy (PMD) for seeking the way to cure muscle retrogression. METHODS: The muscle injured model of 12 C57 male mice was made by injecting cardiotoxin (5 microg per mouse) in left quadriceps femoris, their right quadriceps femoris was used as control without any injection. The histological, immunohistochemical analysis and RT-PCR were done to investigate the expression of CXCR4 in the quadriceps femoris in situ after 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks. RESULTS: HE staining results demonstrated that the muscle tissues experienced the process from muscle injury, repair to regeneration. The result of immunohistochemistry showed that the expressions of CXCR4 in injured muscle tissue were 1955.6 +/- 150.3, 2223.2 +/- 264.3, 2317.6 +/- 178.7, 3066.5 +/- 269.6, 1770.9 +/- 98.7 and 1505.7 +/- 107.1 at 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks after injection of cardiotoxin, there was significant difference when compared with normal muscle (640.3 +/- 124.0, P < 0.00 1). The RT-PCR showed that the expressions of CXCR4 mRNA in injured muscle tissue were 0.822 +/- 0.013, 0.882 +/- 0.025, 1.025 +/- 0.028, 1.065 +/- 0.041, 0.837 +/- 0.011 and 0.777 +/- 0.015 at 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks after injection of cardiotoxin, there was significant difference when compared with normal muscle (0.349 +/- 0.006, P < 0.001). CONCLUSION: CXCR4 may be the critical protein in the process of muscle impairment and reparation.
Persistent Identifierhttp://hdl.handle.net/10722/170144
ISSN
2015 SCImago Journal Rankings: 0.128

 

DC FieldValueLanguage
dc.contributor.authorLi, Xen_US
dc.contributor.authorHuang, Hen_US
dc.contributor.authorZhou, Gen_US
dc.contributor.authorYang, Zen_US
dc.contributor.authorXie, Hen_US
dc.contributor.authorDeng, Len_US
dc.date.accessioned2012-10-30T06:05:35Z-
dc.date.available2012-10-30T06:05:35Z-
dc.date.issued2009en_US
dc.identifier.citationZhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal Of Reparative And Reconstructive Surgery, 2009, v. 23 n. 1, p. 26-29en_US
dc.identifier.issn1002-1892en_US
dc.identifier.urihttp://hdl.handle.net/10722/170144-
dc.description.abstractOBJECTIVE: To observe the expressions of CXC chemokine receptor 4 (CXCR4) in muscle satellite cells in situ of normal and cardiotoxin-intoxicated muscle tissues so as to further investigate the molecular mechanism involving in muscle regeneration such as progressing muscular dystrophy (PMD) for seeking the way to cure muscle retrogression. METHODS: The muscle injured model of 12 C57 male mice was made by injecting cardiotoxin (5 microg per mouse) in left quadriceps femoris, their right quadriceps femoris was used as control without any injection. The histological, immunohistochemical analysis and RT-PCR were done to investigate the expression of CXCR4 in the quadriceps femoris in situ after 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks. RESULTS: HE staining results demonstrated that the muscle tissues experienced the process from muscle injury, repair to regeneration. The result of immunohistochemistry showed that the expressions of CXCR4 in injured muscle tissue were 1955.6 +/- 150.3, 2223.2 +/- 264.3, 2317.6 +/- 178.7, 3066.5 +/- 269.6, 1770.9 +/- 98.7 and 1505.7 +/- 107.1 at 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks after injection of cardiotoxin, there was significant difference when compared with normal muscle (640.3 +/- 124.0, P < 0.00 1). The RT-PCR showed that the expressions of CXCR4 mRNA in injured muscle tissue were 0.822 +/- 0.013, 0.882 +/- 0.025, 1.025 +/- 0.028, 1.065 +/- 0.041, 0.837 +/- 0.011 and 0.777 +/- 0.015 at 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks after injection of cardiotoxin, there was significant difference when compared with normal muscle (0.349 +/- 0.006, P < 0.001). CONCLUSION: CXCR4 may be the critical protein in the process of muscle impairment and reparation.en_US
dc.languageengen_US
dc.relation.ispartofZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgeryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMuscular Diseases - Metabolism - Pathologyen_US
dc.subject.meshReceptors, Cxcr4 - Biosynthesisen_US
dc.subject.meshSatellite Cells, Skeletal Muscle - Metabolismen_US
dc.subject.meshSoft Tissue Injuries - Metabolismen_US
dc.titleExpression of CXC chemokine receptor 4 in muscle satellite cells of muscle injury tissuesen_US
dc.typeArticleen_US
dc.identifier.emailZhou, G:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, G=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid19192873-
dc.identifier.scopuseid_2-s2.0-66849103705en_US
dc.identifier.volume23en_US
dc.identifier.issue1en_US
dc.identifier.spage26en_US
dc.identifier.epage29en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridLi, X=35210528900en_US
dc.identifier.scopusauthoridHuang, H=7405610061en_US
dc.identifier.scopusauthoridZhou, G=23394245100en_US
dc.identifier.scopusauthoridYang, Z=7405433260en_US
dc.identifier.scopusauthoridXie, H=7401672194en_US
dc.identifier.scopusauthoridDeng, L=37001461300en_US

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