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Article: Chondrogenic differentiation alters the immunosuppressive property of bone marrow-derived mesenchymal stem cells, and the effect is partially due to the upregulated expression of B7 molecules

TitleChondrogenic differentiation alters the immunosuppressive property of bone marrow-derived mesenchymal stem cells, and the effect is partially due to the upregulated expression of B7 molecules
Authors
Issue Date2007
PublisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.com
Citation
Stem Cells, 2007, v. 25 n. 2, p. 364-370 How to Cite?
AbstractTo investigate the immunosuppressive properties of MSCs, in the present study we examined the immunogenicity of undifferentiated and trilineage-differentiated (chondrocytes, osteoblasts, and adipocytes) rat bone marrow-derived MSCs under xenogeneic conditions. After chondrogenic differentiation, rat bone marrow-derived MSCs stimulated human dendritic cells (hDCs) derived from peripheral blood monocytes, leading to eight- and fourfold higher lymphocyte proliferation and cytotoxicity than that of undifferentiated MSCs. The chondrogenic-differentiated MSCs were chemotactic to hDCs in Dunn chamber chemotaxis system and were rosetted by hDCs in rosette assays. Flow cytometry analysis revealed that chondrogenic-differentiated MSCs had promoted hDC maturation, causing higher CD83 expression in hDCs, whereas undifferentiated MSCs and osteogenic- and adipogenic-differentiated MSCs showed an inhibitory effect on hDC maturation. The costimulatory B7 molecules were upregulated only in the chondrogenic-differentiated MSCs. After blocking B7 molecules with specific monoclonal antibodies in the chondrogenic-differentiated MSCs, CD83 expression of cocultured hDCs was greatly reduced. In conclusion, chondrogenic differentiation may increase the immunogenicity of MSCs, leading to stimulation of dendritic cells. The upregulated expression of B7 molecules on the chondrogenic-differentiated MSCs may be partially responsible for this event. ©AlphaMed Press.
Persistent Identifierhttp://hdl.handle.net/10722/170100
ISSN
2015 Impact Factor: 5.902
2015 SCImago Journal Rankings: 3.438
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Xen_US
dc.contributor.authorMcclurg, Aen_US
dc.contributor.authorZhou, GQen_US
dc.contributor.authorMccaigue, Men_US
dc.contributor.authorArmstrong, MAen_US
dc.contributor.authorLi, Gen_US
dc.date.accessioned2012-10-30T06:05:18Z-
dc.date.available2012-10-30T06:05:18Z-
dc.date.issued2007en_US
dc.identifier.citationStem Cells, 2007, v. 25 n. 2, p. 364-370en_US
dc.identifier.issn1066-5099en_US
dc.identifier.urihttp://hdl.handle.net/10722/170100-
dc.description.abstractTo investigate the immunosuppressive properties of MSCs, in the present study we examined the immunogenicity of undifferentiated and trilineage-differentiated (chondrocytes, osteoblasts, and adipocytes) rat bone marrow-derived MSCs under xenogeneic conditions. After chondrogenic differentiation, rat bone marrow-derived MSCs stimulated human dendritic cells (hDCs) derived from peripheral blood monocytes, leading to eight- and fourfold higher lymphocyte proliferation and cytotoxicity than that of undifferentiated MSCs. The chondrogenic-differentiated MSCs were chemotactic to hDCs in Dunn chamber chemotaxis system and were rosetted by hDCs in rosette assays. Flow cytometry analysis revealed that chondrogenic-differentiated MSCs had promoted hDC maturation, causing higher CD83 expression in hDCs, whereas undifferentiated MSCs and osteogenic- and adipogenic-differentiated MSCs showed an inhibitory effect on hDC maturation. The costimulatory B7 molecules were upregulated only in the chondrogenic-differentiated MSCs. After blocking B7 molecules with specific monoclonal antibodies in the chondrogenic-differentiated MSCs, CD83 expression of cocultured hDCs was greatly reduced. In conclusion, chondrogenic differentiation may increase the immunogenicity of MSCs, leading to stimulation of dendritic cells. The upregulated expression of B7 molecules on the chondrogenic-differentiated MSCs may be partially responsible for this event. ©AlphaMed Press.en_US
dc.languageengen_US
dc.publisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.comen_US
dc.relation.ispartofStem Cellsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd - Immunologyen_US
dc.subject.meshAntigens, Cd80 - Genetics - Metabolismen_US
dc.subject.meshAntigens, Cd86 - Genetics - Metabolismen_US
dc.subject.meshBone Marrow Cells - Cytology - Immunologyen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineageen_US
dc.subject.meshCell Separationen_US
dc.subject.meshChemotaxisen_US
dc.subject.meshChondrogenesisen_US
dc.subject.meshDendritic Cells - Cytology - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmune Tolerance - Immunologyen_US
dc.subject.meshImmunoglobulins - Immunologyen_US
dc.subject.meshLymphocytes - Cytologyen_US
dc.subject.meshMembrane Glycoproteins - Immunologyen_US
dc.subject.meshMesenchymal Stem Cells - Cytology - Immunologyen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRosette Formationen_US
dc.subject.meshUp-Regulationen_US
dc.titleChondrogenic differentiation alters the immunosuppressive property of bone marrow-derived mesenchymal stem cells, and the effect is partially due to the upregulated expression of B7 moleculesen_US
dc.typeArticleen_US
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, GQ=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1634/stemcells.2006-0268en_US
dc.identifier.pmid17068184-
dc.identifier.scopuseid_2-s2.0-33846933759en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846933759&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume25en_US
dc.identifier.issue2en_US
dc.identifier.spage364en_US
dc.identifier.epage370en_US
dc.identifier.isiWOS:000244070600013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChen, X=8043429800en_US
dc.identifier.scopusauthoridMcClurg, A=6506443164en_US
dc.identifier.scopusauthoridZhou, GQ=23394245100en_US
dc.identifier.scopusauthoridMcCaigue, M=6602143108en_US
dc.identifier.scopusauthoridArmstrong, MA=7202399999en_US
dc.identifier.scopusauthoridLi, G=36013406300en_US

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