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Article: Osteoprecursor cell response to strontium-containing hydorxyapatite ceramics

TitleOsteoprecursor cell response to strontium-containing hydorxyapatite ceramics
Authors
Issue Date2006
Citation
Journal Of Biomedical Materials Research - Part A, 2006, v. 79 n. 4, p. 804-814 How to Cite?
AbstractThe objective of this study was to investigate the in vitro bioactivity of strontium-containing hydroxyapatite (Sr-HA), and its effect on cellular attachment, proliferation, and differentiation. The effect of Sr-HA has been compared with that of hydroxyapatite (HA), which is widely used in orthopedics and dentistry. Sr-HA ceramic containing 10 mol % was prepared. The bioactivity of Sr-HA was evaluated in vitro by immersion in simulated body fluid (SBF). After immersion in SBF, Sr-HA exhibited greater ability to induce apatite precipitation on its surface than did HA. The possible effects on cell behavior of Sr-HA were examined by culturing osteoprecursor cells (OPC1) on materials surfaces. Cell shape and cell-material interactions were analyzed by scanning electron microscope (SEM) and the MTT assay was used to determine cell proliferation on samples. When compared with HA, Sr-HA promoted better OPC1 cell attachment and proliferation, and showed no deleterious effects on extracellular matrix formation and mineralization. Confocal scanning microscopy was used to assess the expression of specific osteoblast proteins: alkaline phosphatase (ALP) and osteopontin (OPN). The results obtained indicate that the presence of Sr stimulates OPC1 cell differentiation, and enhances ALP and OPN expression. © 2006 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/170096
ISSN
2004 Impact Factor: 3.652
2006 SCImago Journal Rankings: 0.474
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXue, Wen_US
dc.contributor.authorMoore, JLen_US
dc.contributor.authorHosick, HLen_US
dc.contributor.authorBose, Sen_US
dc.contributor.authorBandyopadhyay, Aen_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorCheung, KMCen_US
dc.contributor.authorLuk, KDKen_US
dc.date.accessioned2012-10-30T06:05:17Z-
dc.date.available2012-10-30T06:05:17Z-
dc.date.issued2006en_US
dc.identifier.citationJournal Of Biomedical Materials Research - Part A, 2006, v. 79 n. 4, p. 804-814en_US
dc.identifier.issn0021-9304en_US
dc.identifier.urihttp://hdl.handle.net/10722/170096-
dc.description.abstractThe objective of this study was to investigate the in vitro bioactivity of strontium-containing hydroxyapatite (Sr-HA), and its effect on cellular attachment, proliferation, and differentiation. The effect of Sr-HA has been compared with that of hydroxyapatite (HA), which is widely used in orthopedics and dentistry. Sr-HA ceramic containing 10 mol % was prepared. The bioactivity of Sr-HA was evaluated in vitro by immersion in simulated body fluid (SBF). After immersion in SBF, Sr-HA exhibited greater ability to induce apatite precipitation on its surface than did HA. The possible effects on cell behavior of Sr-HA were examined by culturing osteoprecursor cells (OPC1) on materials surfaces. Cell shape and cell-material interactions were analyzed by scanning electron microscope (SEM) and the MTT assay was used to determine cell proliferation on samples. When compared with HA, Sr-HA promoted better OPC1 cell attachment and proliferation, and showed no deleterious effects on extracellular matrix formation and mineralization. Confocal scanning microscopy was used to assess the expression of specific osteoblast proteins: alkaline phosphatase (ALP) and osteopontin (OPN). The results obtained indicate that the presence of Sr stimulates OPC1 cell differentiation, and enhances ALP and OPN expression. © 2006 Wiley Periodicals, Inc.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Biomedical Materials Research - Part Aen_US
dc.rightsJournal of Biomedical Materials Research Part A. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshAlkaline Phosphatase - Biosynthesisen_US
dc.subject.meshCalcification, Physiologicen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCeramics - Chemistryen_US
dc.subject.meshCoated Materials, Biocompatible - Chemistryen_US
dc.subject.meshDurapatite - Chemistryen_US
dc.subject.meshHumansen_US
dc.subject.meshOsteoblasts - Metabolism - Ultrastructureen_US
dc.subject.meshOsteopontin - Biosynthesisen_US
dc.subject.meshStem Cells - Metabolism - Ultrastructureen_US
dc.subject.meshStrontium - Chemistryen_US
dc.titleOsteoprecursor cell response to strontium-containing hydorxyapatite ceramicsen_US
dc.typeArticleen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_US
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.identifier.authorityCheung, KMC=rp00387en_US
dc.identifier.authorityLuk, KDK=rp00333en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jbm.a.30815en_US
dc.identifier.pmid16886220-
dc.identifier.scopuseid_2-s2.0-33845501381en_US
dc.identifier.hkuros145161-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845501381&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume79en_US
dc.identifier.issue4en_US
dc.identifier.spage804en_US
dc.identifier.epage814en_US
dc.identifier.isiWOS:000242360400005-
dc.identifier.scopusauthoridXue, W=7103165239en_US
dc.identifier.scopusauthoridMoore, JL=37086858400en_US
dc.identifier.scopusauthoridHosick, HL=7004832024en_US
dc.identifier.scopusauthoridBose, S=7401626559en_US
dc.identifier.scopusauthoridBandyopadhyay, A=35582612800en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US
dc.identifier.scopusauthoridCheung, KMC=7402406754en_US
dc.identifier.scopusauthoridLuk, KDK=7201921573en_US

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