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Article: Gene expression profile of degenerated cervical intervertebral disc tissues in rats

TitleGene expression profile of degenerated cervical intervertebral disc tissues in rats
Authors
KeywordsGene Expression
Intervertebral Disc
Rats
Issue Date2004
Citation
Chinese Journal Of Traumatology - English Edition, 2004, v. 7 n. 6, p. 330-340 How to Cite?
AbstractObjective: To analyze the gene expression profile of degenerated cervical intervertebral disc of Sprague Dawley rats on a large scale. Methods: Degenerated models of Sprague Dawley rats of 9 months old (degeneration group, n = 9) and normal Sprague Dawley rats of 3 months old (control group, n = 9) were prepared, respectively. mRNA was obtained from the cervical intervertebral disc of rats in both groups, respectively, and then labelled by Cy5 and Cy3 fluorescence respectively after reverse transcription to obtain intervertebral disc cDNA probes. cDNA probes were hybridized with BiostarR-40s gene expression profile chips and scanned by laser scanner. The results were treated with portrait analysis, standardization management, and ratio analysis with softwares. Results: Compared with the rats in the control group, 9.6% (381 pieces in total) gene expression changed obviously in the rats in the degeneration group, among which, the gene expression quantities of 171 pieces increased significantly (r = the ratio of the degeneration group to the control group > 2.0), 52 pieces of which had certain function. While the gene expression quantities of 211 pieces decreased significantly (r < 0.5), 41 pieces of which had certain function. Conclusions: Gene chip technology can be used to analyze the gene expression profile of degenerated intervertebral disc of rats in parallel, in quantity and on a large scale, which helps to testify the representative genes and protein expression, and plays an important role in clarifying the pathogenesis of degenerated intervertebral disc.
Persistent Identifierhttp://hdl.handle.net/10722/170067
ISSN
2015 SCImago Journal Rankings: 0.183
References

 

DC FieldValueLanguage
dc.contributor.authorWang, YJen_US
dc.contributor.authorLü, WJen_US
dc.contributor.authorShi, Qen_US
dc.contributor.authorZhou, CJen_US
dc.contributor.authorZhang, Qen_US
dc.contributor.authorZhou, Qen_US
dc.contributor.authorBian, Qen_US
dc.contributor.authorLiu, Men_US
dc.contributor.authorLiang, ZRen_US
dc.date.accessioned2012-10-30T06:05:07Z-
dc.date.available2012-10-30T06:05:07Z-
dc.date.issued2004en_US
dc.identifier.citationChinese Journal Of Traumatology - English Edition, 2004, v. 7 n. 6, p. 330-340en_US
dc.identifier.issn1008-1275en_US
dc.identifier.urihttp://hdl.handle.net/10722/170067-
dc.description.abstractObjective: To analyze the gene expression profile of degenerated cervical intervertebral disc of Sprague Dawley rats on a large scale. Methods: Degenerated models of Sprague Dawley rats of 9 months old (degeneration group, n = 9) and normal Sprague Dawley rats of 3 months old (control group, n = 9) were prepared, respectively. mRNA was obtained from the cervical intervertebral disc of rats in both groups, respectively, and then labelled by Cy5 and Cy3 fluorescence respectively after reverse transcription to obtain intervertebral disc cDNA probes. cDNA probes were hybridized with BiostarR-40s gene expression profile chips and scanned by laser scanner. The results were treated with portrait analysis, standardization management, and ratio analysis with softwares. Results: Compared with the rats in the control group, 9.6% (381 pieces in total) gene expression changed obviously in the rats in the degeneration group, among which, the gene expression quantities of 171 pieces increased significantly (r = the ratio of the degeneration group to the control group > 2.0), 52 pieces of which had certain function. While the gene expression quantities of 211 pieces decreased significantly (r < 0.5), 41 pieces of which had certain function. Conclusions: Gene chip technology can be used to analyze the gene expression profile of degenerated intervertebral disc of rats in parallel, in quantity and on a large scale, which helps to testify the representative genes and protein expression, and plays an important role in clarifying the pathogenesis of degenerated intervertebral disc.en_US
dc.languageengen_US
dc.relation.ispartofChinese Journal of Traumatology - English Editionen_US
dc.subjectGene Expressionen_US
dc.subjectIntervertebral Discen_US
dc.subjectRatsen_US
dc.titleGene expression profile of degenerated cervical intervertebral disc tissues in ratsen_US
dc.typeArticleen_US
dc.identifier.emailLü, WJ:wwlu@hku.hken_US
dc.identifier.authorityLü, WJ=rp00411en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-20444379665en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20444379665&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume7en_US
dc.identifier.issue6en_US
dc.identifier.spage330en_US
dc.identifier.epage340en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWang, YJ=8320879700en_US
dc.identifier.scopusauthoridLü, WJ=7404215221en_US
dc.identifier.scopusauthoridShi, Q=7402698372en_US
dc.identifier.scopusauthoridZhou, CJ=7403347036en_US
dc.identifier.scopusauthoridZhang, Q=35546936300en_US
dc.identifier.scopusauthoridZhou, Q=52465006800en_US
dc.identifier.scopusauthoridBian, Q=8454459900en_US
dc.identifier.scopusauthoridLiu, M=55210644900en_US
dc.identifier.scopusauthoridLiang, ZR=8239417400en_US

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