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Article: An investigation on the physicochemical properties of chitosan/DNA polyelectrolyte complexes

TitleAn investigation on the physicochemical properties of chitosan/DNA polyelectrolyte complexes
Authors
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2005, v. 26 n. 15, p. 2705-2711 How to Cite?
AbstractIn this work, to eliminate the effect of the hydrophobicity of N-acetyl groups in chitosan on the interaction between chitosan and DNA, a water soluble chitosan with molecular weight of 5000 and deacetylated degree of 99% was selected to complex with DNA at varied charged ratios. The physicochemical properties of chitoplexes were investigated by means of FTIR, circular dichroism (CD), static fluorescence spectroscopy, and atomic force microscopy (AFM). The results indicated that upon interacting with chitosan, the DNA molecules saved a B conformation, and the binding affinity of chitosan to DNA was dependent on pH of media. At pH 5.5, highly charged chitosan had a strong binding affinity with DNA; whereas in pH 12.0 medium, only weak interactions existed. The CD spectra of Hoechst 33258 competitive displacement revealed that chitosan was partially bound to the minor groove of DNA. The morphology of chitosan/DNA complexes was strongly dependent upon the charge ratios. At charge ratio (+/-) of 1:4, not all DNA could be entrapped in the complex; at ratio of 8:1, the spherical complexes with mean size of nanoscale were formed without free DNA, but no typical toroid patterns were observed, which might stem from the strong compact of DNA caused by highly charged chitosan. It was supposed that the strong interaction of chitosan with DNA possibly prevented gene unpacking from chitosan vector, consequently restraining gene expression in nucleus. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/170056
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Wen_US
dc.contributor.authorSun, Sen_US
dc.contributor.authorCao, Zen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorYao, Ken_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorLuk, KDKen_US
dc.date.accessioned2012-10-30T06:05:02Z-
dc.date.available2012-10-30T06:05:02Z-
dc.date.issued2005en_US
dc.identifier.citationBiomaterials, 2005, v. 26 n. 15, p. 2705-2711en_US
dc.identifier.issn0142-9612en_US
dc.identifier.urihttp://hdl.handle.net/10722/170056-
dc.description.abstractIn this work, to eliminate the effect of the hydrophobicity of N-acetyl groups in chitosan on the interaction between chitosan and DNA, a water soluble chitosan with molecular weight of 5000 and deacetylated degree of 99% was selected to complex with DNA at varied charged ratios. The physicochemical properties of chitoplexes were investigated by means of FTIR, circular dichroism (CD), static fluorescence spectroscopy, and atomic force microscopy (AFM). The results indicated that upon interacting with chitosan, the DNA molecules saved a B conformation, and the binding affinity of chitosan to DNA was dependent on pH of media. At pH 5.5, highly charged chitosan had a strong binding affinity with DNA; whereas in pH 12.0 medium, only weak interactions existed. The CD spectra of Hoechst 33258 competitive displacement revealed that chitosan was partially bound to the minor groove of DNA. The morphology of chitosan/DNA complexes was strongly dependent upon the charge ratios. At charge ratio (+/-) of 1:4, not all DNA could be entrapped in the complex; at ratio of 8:1, the spherical complexes with mean size of nanoscale were formed without free DNA, but no typical toroid patterns were observed, which might stem from the strong compact of DNA caused by highly charged chitosan. It was supposed that the strong interaction of chitosan with DNA possibly prevented gene unpacking from chitosan vector, consequently restraining gene expression in nucleus. © 2004 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_US
dc.relation.ispartofBiomaterialsen_US
dc.rightsBiomaterials. Copyright © Elsevier BV.-
dc.subject.meshChitosan - Analysis - Chemistryen_US
dc.subject.meshCoated Materials, Biocompatible - Analysis - Chemistryen_US
dc.subject.meshDna - Analysis - Chemistry - Ultrastructureen_US
dc.subject.meshDrug Delivery Systems - Methodsen_US
dc.subject.meshElectrolytes - Chemistryen_US
dc.subject.meshGene Transfer Techniquesen_US
dc.subject.meshMaterials Testingen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshNanotubes - Analysis - Chemistry - Ultrastructureen_US
dc.subject.meshParticle Sizeen_US
dc.subject.meshTransfection - Methodsen_US
dc.titleAn investigation on the physicochemical properties of chitosan/DNA polyelectrolyte complexesen_US
dc.typeArticleen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.identifier.authorityLuk, KDK=rp00333en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.biomaterials.2004.07.038en_US
dc.identifier.pmid15585274-
dc.identifier.scopuseid_2-s2.0-10044287286en_US
dc.identifier.hkuros114396-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10044287286&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume26en_US
dc.identifier.issue15en_US
dc.identifier.spage2705en_US
dc.identifier.epage2711en_US
dc.identifier.isiWOS:000226698400054-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLiu, W=7408473555en_US
dc.identifier.scopusauthoridSun, S=7404509581en_US
dc.identifier.scopusauthoridCao, Z=55225316600en_US
dc.identifier.scopusauthoridZhang, X=36065164400en_US
dc.identifier.scopusauthoridYao, K=7403234424en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US
dc.identifier.scopusauthoridLuk, KDK=7201921573en_US
dc.identifier.citeulike4884989-

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