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Article: Both substance P and its receptor are expressed in mouse intestinal T lymphocytes

TitleBoth substance P and its receptor are expressed in mouse intestinal T lymphocytes
Authors
Issue Date2001
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NEN
Citation
Neuroendocrinology, 2001, v. 73 n. 5, p. 358-368 How to Cite?
AbstractSubstance P (SP), one of the most prevalent neuropeptides in gut, has been reported to have potent immune modulatory effects as a proinflammatory agent. The synthesis of SP and SP receptor expression in intraepithelial and lamina propria T lymphocytes of mouse intestine was investigated. Using RT-PCR analysis, it was demonstrated that SP receptor mRNA was exclusively expressed in intraepithelial and lamina propria T lymphocytes as well as their purified CD4+, CD8+ and CD4-CD8-CD3+ subsets. Messenger RNAs (mRNAs) for the two precursors of SP, β and γ-preprotachykinin-A, were also detected. These results were consistent in lymphocytes from both epithelium and lamina propria of small and large intestines, although the frequencies and/or intensities of mRNA expression varied. However, none of the findings could be repeated in splenic T lymphocytes. Activation of splenocytes with anti-CD3ε-chain mAb and PMA did not induce expression of SP or its receptor mRNAs. Furthermore, both cytoplasmic and surface-bound SP was demonstrated in intestinal T lymphocytes using dual color immunocytochemistry and immunoflow cytometry. In vitro treatment with SP did not significantly change the size of the SP-immunoreactive T cell population, indicating the presence of SP receptor on intestinal T lymphocytes as well as in vivo binding of endogenously released SP. Our data suggest that SP production and SP receptor expression are distinctive for mouse intestinal mucosal immunity and that SP may act as a modulator of an ongoing controlled inflammation in normal gut, by acting through its specific receptor on T lymphocytes in an autocrine and/or paracrine pattern. Copyright © 2001 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/170032
ISSN
2015 Impact Factor: 2.583
2015 SCImago Journal Rankings: 1.479
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQian, BFen_US
dc.contributor.authorZhou, GQen_US
dc.contributor.authorHammarström, MLen_US
dc.contributor.authorDanielsson, Åen_US
dc.date.accessioned2012-10-30T06:04:51Z-
dc.date.available2012-10-30T06:04:51Z-
dc.date.issued2001en_US
dc.identifier.citationNeuroendocrinology, 2001, v. 73 n. 5, p. 358-368en_US
dc.identifier.issn0028-3835en_US
dc.identifier.urihttp://hdl.handle.net/10722/170032-
dc.description.abstractSubstance P (SP), one of the most prevalent neuropeptides in gut, has been reported to have potent immune modulatory effects as a proinflammatory agent. The synthesis of SP and SP receptor expression in intraepithelial and lamina propria T lymphocytes of mouse intestine was investigated. Using RT-PCR analysis, it was demonstrated that SP receptor mRNA was exclusively expressed in intraepithelial and lamina propria T lymphocytes as well as their purified CD4+, CD8+ and CD4-CD8-CD3+ subsets. Messenger RNAs (mRNAs) for the two precursors of SP, β and γ-preprotachykinin-A, were also detected. These results were consistent in lymphocytes from both epithelium and lamina propria of small and large intestines, although the frequencies and/or intensities of mRNA expression varied. However, none of the findings could be repeated in splenic T lymphocytes. Activation of splenocytes with anti-CD3ε-chain mAb and PMA did not induce expression of SP or its receptor mRNAs. Furthermore, both cytoplasmic and surface-bound SP was demonstrated in intestinal T lymphocytes using dual color immunocytochemistry and immunoflow cytometry. In vitro treatment with SP did not significantly change the size of the SP-immunoreactive T cell population, indicating the presence of SP receptor on intestinal T lymphocytes as well as in vivo binding of endogenously released SP. Our data suggest that SP production and SP receptor expression are distinctive for mouse intestinal mucosal immunity and that SP may act as a modulator of an ongoing controlled inflammation in normal gut, by acting through its specific receptor on T lymphocytes in an autocrine and/or paracrine pattern. Copyright © 2001 S. Karger AG, Basel.en_US
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NENen_US
dc.relation.ispartofNeuroendocrinologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd3 - Analysisen_US
dc.subject.meshCd4-Positive T-Lymphocytes - Chemistryen_US
dc.subject.meshCd8-Positive T-Lymphocytes - Chemistryen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIntestines - Cytologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshProtein Precursors - Geneticsen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshReceptors, Neurokinin-1 - Geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSubstance P - Analysis - Geneticsen_US
dc.subject.meshT-Lymphocytes - Chemistry - Immunologyen_US
dc.subject.meshTachykinins - Geneticsen_US
dc.subject.meshTetradecanoylphorbol Acetate - Pharmacologyen_US
dc.titleBoth substance P and its receptor are expressed in mouse intestinal T lymphocytesen_US
dc.typeArticleen_US
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, GQ=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000054653en_US
dc.identifier.pmid11399909-
dc.identifier.scopuseid_2-s2.0-0034955149en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034955149&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume73en_US
dc.identifier.issue5en_US
dc.identifier.spage358en_US
dc.identifier.epage368en_US
dc.identifier.isiWOS:000169288900008-
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridQian, BF=7102420669en_US
dc.identifier.scopusauthoridZhou, GQ=23394245100en_US
dc.identifier.scopusauthoridHammarström, ML=35588604400en_US
dc.identifier.scopusauthoridDanielsson, Å=7101921575en_US

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