File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/S0378-1119(98)00550-2
- Scopus: eid_2-s2.0-0033534795
- PMID: 10048958
- WOS: WOS:000078250400008
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Caenorhabditis elegans ZC376.5 encodes a tRNA(m2/2g26)dimethyltransferase in which 246arginine is important for the enzyme activity
Title | Caenorhabditis elegans ZC376.5 encodes a tRNA(m2/2g26)dimethyltransferase in which 246arginine is important for the enzyme activity |
---|---|
Authors | |
Keywords | CDNA cloning Eukaryote Modification Mutagenesis TRM1 |
Issue Date | 1999 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene |
Citation | Gene, 1999, v. 226 n. 1, p. 73-81 How to Cite? |
Abstract | It has been estimated that eukaryotes carry more than 50 genes for tRNA modifying enzymes. Of the few so far identified most come from yeast, a lower eukaryote. In Saccharomyces cerevisiae, the TRM1 gene is a nuclear gene encoding the tRNA(m2 2G26)dimethyltransferase, which catalyses the formation of the N2, N2-dimethylguanosine at position 26 in tRNA. We have isolated and characterized the corresponding gene ZC376.5 in Caenorhabditis elegans. Via RT-PCR the cDNA sequence of the full length ZC376.5 has now been cloned, expressed in Escherichia coli and demonstrated to encode a tRNA(m2 2G26)dimethyltransferase that produces dimethyl-G26 in vivo and in vitro with tRNA from yeast and bacteria as substrates. This is the first example of a complete gene sequence coding for a tRNA modifying enzyme from a multicellular organism. A point mutation in exon IV in the C. elegans genome sequence coding for the tRNA(m2 2G26)methyltransferase that substituted arginine246 for glycine eliminated the modification activity. Exchanging the corresponding lysine residue in the yeast Trm1p for alanine caused a severe loss of activity, indicating that the identity of the amino acid at this position is important for enzyme activity. |
Persistent Identifier | http://hdl.handle.net/10722/170022 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.725 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, J | en_US |
dc.contributor.author | Zhou, GQ | en_US |
dc.contributor.author | Stråby, KB | en_US |
dc.date.accessioned | 2012-10-30T06:04:48Z | - |
dc.date.available | 2012-10-30T06:04:48Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Gene, 1999, v. 226 n. 1, p. 73-81 | en_US |
dc.identifier.issn | 0378-1119 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/170022 | - |
dc.description.abstract | It has been estimated that eukaryotes carry more than 50 genes for tRNA modifying enzymes. Of the few so far identified most come from yeast, a lower eukaryote. In Saccharomyces cerevisiae, the TRM1 gene is a nuclear gene encoding the tRNA(m2 2G26)dimethyltransferase, which catalyses the formation of the N2, N2-dimethylguanosine at position 26 in tRNA. We have isolated and characterized the corresponding gene ZC376.5 in Caenorhabditis elegans. Via RT-PCR the cDNA sequence of the full length ZC376.5 has now been cloned, expressed in Escherichia coli and demonstrated to encode a tRNA(m2 2G26)dimethyltransferase that produces dimethyl-G26 in vivo and in vitro with tRNA from yeast and bacteria as substrates. This is the first example of a complete gene sequence coding for a tRNA modifying enzyme from a multicellular organism. A point mutation in exon IV in the C. elegans genome sequence coding for the tRNA(m2 2G26)methyltransferase that substituted arginine246 for glycine eliminated the modification activity. Exchanging the corresponding lysine residue in the yeast Trm1p for alanine caused a severe loss of activity, indicating that the identity of the amino acid at this position is important for enzyme activity. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene | en_US |
dc.relation.ispartof | Gene | en_US |
dc.subject | CDNA cloning | - |
dc.subject | Eukaryote | - |
dc.subject | Modification | - |
dc.subject | Mutagenesis | - |
dc.subject | TRM1 | - |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Arginine - Metabolism | en_US |
dc.subject.mesh | Caenorhabditis Elegans - Enzymology - Genetics | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Helminth Proteins - Genetics - Isolation & Purification - Metabolism | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Mutation | en_US |
dc.subject.mesh | Recombinant Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Sequence Homology, Amino Acid | en_US |
dc.subject.mesh | Trna Methyltransferases - Genetics - Isolation & Purification - Metabolism | en_US |
dc.title | Caenorhabditis elegans ZC376.5 encodes a tRNA(m2/2g26)dimethyltransferase in which 246arginine is important for the enzyme activity | en_US |
dc.type | Article | en_US |
dc.identifier.email | Zhou, GQ:wormoscz@gmail.com | en_US |
dc.identifier.authority | Zhou, GQ=rp00527 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0378-1119(98)00550-2 | en_US |
dc.identifier.pmid | 10048958 | - |
dc.identifier.scopus | eid_2-s2.0-0033534795 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033534795&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 226 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 73 | en_US |
dc.identifier.epage | 81 | en_US |
dc.identifier.isi | WOS:000078250400008 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Liu, J=36077397200 | en_US |
dc.identifier.scopusauthorid | Zhou, GQ=23394245100 | en_US |
dc.identifier.scopusauthorid | Stråby, KB=6603368582 | en_US |
dc.identifier.issnl | 0378-1119 | - |