File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Caenorhabditis elegans ZC376.5 encodes a tRNA(m2/2g26)dimethyltransferase in which 246arginine is important for the enzyme activity

TitleCaenorhabditis elegans ZC376.5 encodes a tRNA(m2/2g26)dimethyltransferase in which 246arginine is important for the enzyme activity
Authors
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 1999, v. 226 n. 1, p. 73-81 How to Cite?
AbstractIt has been estimated that eukaryotes carry more than 50 genes for tRNA modifying enzymes. Of the few so far identified most come from yeast, a lower eukaryote. In Saccharomyces cerevisiae, the TRM1 gene is a nuclear gene encoding the tRNA(m2 2G26)dimethyltransferase, which catalyses the formation of the N2, N2-dimethylguanosine at position 26 in tRNA. We have isolated and characterized the corresponding gene ZC376.5 in Caenorhabditis elegans. Via RT-PCR the cDNA sequence of the full length ZC376.5 has now been cloned, expressed in Escherichia coli and demonstrated to encode a tRNA(m2 2G26)dimethyltransferase that produces dimethyl-G26 in vivo and in vitro with tRNA from yeast and bacteria as substrates. This is the first example of a complete gene sequence coding for a tRNA modifying enzyme from a multicellular organism. A point mutation in exon IV in the C. elegans genome sequence coding for the tRNA(m2 2G26)methyltransferase that substituted arginine246 for glycine eliminated the modification activity. Exchanging the corresponding lysine residue in the yeast Trm1p for alanine caused a severe loss of activity, indicating that the identity of the amino acid at this position is important for enzyme activity.
Persistent Identifierhttp://hdl.handle.net/10722/170022
ISSN
2015 Impact Factor: 2.319
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Jen_US
dc.contributor.authorZhou, GQen_US
dc.contributor.authorStråby, KBen_US
dc.date.accessioned2012-10-30T06:04:48Z-
dc.date.available2012-10-30T06:04:48Z-
dc.date.issued1999en_US
dc.identifier.citationGene, 1999, v. 226 n. 1, p. 73-81en_US
dc.identifier.issn0378-1119en_US
dc.identifier.urihttp://hdl.handle.net/10722/170022-
dc.description.abstractIt has been estimated that eukaryotes carry more than 50 genes for tRNA modifying enzymes. Of the few so far identified most come from yeast, a lower eukaryote. In Saccharomyces cerevisiae, the TRM1 gene is a nuclear gene encoding the tRNA(m2 2G26)dimethyltransferase, which catalyses the formation of the N2, N2-dimethylguanosine at position 26 in tRNA. We have isolated and characterized the corresponding gene ZC376.5 in Caenorhabditis elegans. Via RT-PCR the cDNA sequence of the full length ZC376.5 has now been cloned, expressed in Escherichia coli and demonstrated to encode a tRNA(m2 2G26)dimethyltransferase that produces dimethyl-G26 in vivo and in vitro with tRNA from yeast and bacteria as substrates. This is the first example of a complete gene sequence coding for a tRNA modifying enzyme from a multicellular organism. A point mutation in exon IV in the C. elegans genome sequence coding for the tRNA(m2 2G26)methyltransferase that substituted arginine246 for glycine eliminated the modification activity. Exchanging the corresponding lysine residue in the yeast Trm1p for alanine caused a severe loss of activity, indicating that the identity of the amino acid at this position is important for enzyme activity.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_US
dc.relation.ispartofGeneen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArginine - Metabolismen_US
dc.subject.meshCaenorhabditis Elegans - Enzymology - Geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshHelminth Proteins - Genetics - Isolation & Purification - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutationen_US
dc.subject.meshRecombinant Proteins - Genetics - Metabolismen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshTrna Methyltransferases - Genetics - Isolation & Purification - Metabolismen_US
dc.titleCaenorhabditis elegans ZC376.5 encodes a tRNA(m2/2g26)dimethyltransferase in which 246arginine is important for the enzyme activityen_US
dc.typeArticleen_US
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_US
dc.identifier.authorityZhou, GQ=rp00527en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0378-1119(98)00550-2en_US
dc.identifier.pmid10048958-
dc.identifier.scopuseid_2-s2.0-0033534795en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033534795&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume226en_US
dc.identifier.issue1en_US
dc.identifier.spage73en_US
dc.identifier.epage81en_US
dc.identifier.isiWOS:000078250400008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLiu, J=36077397200en_US
dc.identifier.scopusauthoridZhou, GQ=23394245100en_US
dc.identifier.scopusauthoridStråby, KB=6603368582en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats